A flow cytometer (FACSCalibur, Becton-Dickinson, USA) was employed for cell apoptosis assay using Annexin V-APC/7-AAD Apoptosis Assays Kit (KGA1024, KeyGen BioTECH, Nanjing, China). For cell cycle analysis, a PI single staining method was implemented followed by the flow cytometry analysis using Cell Cycle Assay Kit (KGA511, KeyGen BioTECH, Nanjing, China).
Annexin 5 apc 7 aad apoptosis assays kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin V-APC/7-AAD Apoptosis Assays Kit is a laboratory equipment used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and 7-AAD, a DNA-binding dye, to differentiate between early apoptotic, late apoptotic, and necrotic cells.
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Lab products found in correlation
2 protocols using annexin 5 apc 7 aad apoptosis assays kit
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Cell Apoptosis and Cell Cycle Analysis
2
Cell Cycle and Apoptosis Assays
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For cell cycle assays, cells were harvested, fixed in 70% ethanol, stained in 500 μL phosphate‐buffered saline (PBS) containing 50 μg/mL propidium iodide and 100 μg/mL RNase A. After incubation, the cell cycle distribution was measured using a flow cytometer (Beckman, Brea, CA, USA).
For cell apoptosis assays, cells were harvested, washed with PBS, and then stained using an Annexin V‐APC/7‐AAD Apoptosis Assays Kit (KeyGEN Biotech, Nanjing, China) according to the manufacturer's instructions. Finally, apoptosis was analyzed by flow cytometry.
For cell apoptosis assays, cells were harvested, washed with PBS, and then stained using an Annexin V‐APC/7‐AAD Apoptosis Assays Kit (KeyGEN Biotech, Nanjing, China) according to the manufacturer's instructions. Finally, apoptosis was analyzed by flow cytometry.
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