Single cell suspensions of total splenocytes, enriched B cells or BM were stained with different combinations of the following antibodies: Anti-CD4 APC-eFluor 780, anti-CD8 APC-eFluor 780, anti-Gr1 APC-eFluor 780, anti-F4/80 APC-eFluor 780, anti-B220 APC, anti-B220 APC-eFlour 780, anti-B220 FITC, anti CD19 PeCy7, anti-CD38 Alexa Fluor 700, anti-CD93 APC, anti-IgM PerCP-eFluor 710, anti CD21/CD35 eFluor 450 (eBiosciences), anti-CD23 PE (BioLegend), anti-CD4 PE-CF594, anti-CD8 PE-CF594, anti-Ly-6G and Ly-6C PE-CF594 and anti-IgG1 BV421 (BD biosciences). Live dead aqua stain was added to separate dead cells (Life Technologies) and eOD-GT8-specific cells were visualized by the addition of FITC-conjugated eOD-GT8 and PE-conjugated eOD-GT8 CD4bs knock-out. BG505 SOSIP- (Sok et al., 2014 (link)) and 2cc-specific memory B cells were visualized by the addition of biotinylated protein with the addition of streptavidin conjugated PE and APC respectively (BD Biosicences).
Anti cd8 pe cf594
Manufactured by BD
Sourced in United States
Anti-CD8-PE-CF594 is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 antigen, which is expressed on the surface of a subset of T lymphocytes. This product is intended for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd4 fitc, by BD (3 mentions)
Anti cd3 pe cy5, by BD (3 mentions)
Anti cd56 pe cy7, by BD (3 mentions)
Cytomics fc500 flow cytometer, by Beckman Coulter (2 mentions)
Cxp analysis software, by Beckman Coulter (2 mentions)
Anti cd8 apc efluor780, by Thermo Fisher Scientific (1 mentions)
Anti cd4 apc efluor 780, by Thermo Fisher Scientific (1 mentions)
Anti b220 fitc, by Thermo Fisher Scientific (1 mentions)
Anti b220 apc, by Thermo Fisher Scientific (1 mentions)
Aqua live dead stain, by Thermo Fisher Scientific (1 mentions)
Anti cd19 pecy7, by Thermo Fisher Scientific (1 mentions)
Anti cd38 alexa fluor 700, by Thermo Fisher Scientific (1 mentions)
Anti cd23 pe, by BioLegend (1 mentions)
Anti igg1 bv421, by BD (1 mentions)
Anti cd4 pecf594, by BD (1 mentions)
Anti b220 apceflour780, by Thermo Fisher Scientific (1 mentions)
Anti cd25 apc, by BD (1 mentions)
Anti cd62l fitc, by BD (1 mentions)
Anti cd45ro apc, by BD (1 mentions)
Anti pd 1 pe, by BD (1 mentions)
4 protocols using anti cd8 pe cf594
1
Multiparametric Flow Cytometry of Immune Cell Subsets
2
Phenotyping Autologous CIK Cells
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The phenotype of the autologous CIK cells was characterized by flow cytometry. CIK cells were resuspended at 2 × 105 cells per 100 μL of phosphate-buffered saline (PBS) and incubated for 30 min at 4°C with the following anti-human antibodies: anti-CD3-PE-Cy5, anti-CD4-FITC, anti-CD8-PE-CF594 and anti-CD56-PE-Cy7 (all from BD Bioscicence). The cells were analyzed using a CytomicsTM FC500 Flow Cytometer (Beckman Coulter, USA). Data analysis was performed with CXP analysis software (Beckman Coulter, USA).
3
Flow Cytometric Analysis of CIK Cells
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CIK cells were resuspended at 2 × 105 cells per 100 μL of phosphate-buffered saline (PBS) and incubated for 30 min at 4 °C with the following anti-human antibodies: anti-CD3-PE-Cy5, anti-CD4-FITC, anti-CD8-PE-CF594, anti-CD25-APC, anti-CD56-PE-Cy7, anti-CD45RO-APC, and anti-CD62L-FITC (all from BD Bioscicence). The cells were analyzed using a CytomicsTM FC500 Flow Cytometer (Beckman Coulter, USA). Data analysis was performed with CXP analysis software (Beckman Coulter, USA).
4
Characterization of PD-1 Expression on Autologous CIK Cells
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PD-1 expression of the autologous CIK cells from five ECO patients was characterized by flow cytometry using anti-CD3-PE-Cy5, anti-CD4-FITC, anti-CD8-PECF594, anti-CD56- PE-Cy7, and anti-PD-1-PE (BD Bioscicence CA, USA). The ratio of each subgroup was calculated based on the cell density of each gate.
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