U2932

Manufactured by American Type Culture Collection

Sourced in United States

The U2932 is a laboratory equipment product from American Type Culture Collection. It is designed for the cultivation and maintenance of cell cultures. The core function of the U2932 is to provide a controlled and stable environment for cell growth and proliferation.

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U2932 (ABC-DLBCL) (3 citations)

24 protocols using u2932

Comprehensive DLBCL Characterization and Cell Line Validation

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This study was approved by the Medical Ethical Committee of Shandong Provincial Hospital, and written informed consent from each patient and volunteer was conformed to the Declaration of Helsinki. Histological diagnoses in accordance with the 2016 WHO classification were established [67 (link)]. Serum and peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of DLBCL patients and healthy donors from 2017 to 2019. CD19⁺ B cells were purified from freshly isolated PBMCs of healthy donors. The clinical information was collected in the database of Shandong Provincial Hospital. LY1, LY3, LY8, LY10, VAL, U2932, SU-DHL-2 cells were bought from ATCC, cultured in Iscove modified Dulbecco medium (IMDM, Gibco, CA, USA) enriched with 10% heat-inactivated fetal bovine serum (HyClone, UT, USA), 1% penicillin/streptomycin mixture and 2 mM glutamine, and incubated at 37 °C and 5% CO₂. All cells were periodically examined for mycoplasma infection and STR (Short Tandem Repeat).

Hu S., Lu T., Shang J., Cai Y., Ding M., Zhou X, & Wang X. (2023). PGD2 displays distinct effects in diffuse large B-cell lymphoma depending on different concentrations. Cell Death Discovery, 9, 39.

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Cell Culture Conditions and Preparation

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Cells were grown at 37 °C and 5% ambient CO₂. HeLa Kyoto (ATCC), MDA-MB-231 (ATCC), and A549 cells (ATCC) were grown to ~80% confluence in 140 × 20 mm dishes (Nunclon^TM Delta Airvent, Thermo Fisher) with Dulbecco’s Modified Eagle’s Medium, high glucose, GlutaMAX^TM supplement (DMEM, Thermo Scientific), 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin. H-358 (ATCC CRL-5807) and U-2932 (ATCC) cell lines were cultured in RPMI 1640 with GlutaMAX^TM, 1% penicillin-streptomycin, and 10% FBS. Prior to HATRIC-LRC experiments with folate, HeLa cells were starved for 48 h in folate-free RPMI (Gibco).

Sobotzki N., Schafroth M.A., Rudnicka A., Koetemann A., Marty F., Goetze S., Yamauchi Y., Carreira E.M, & Wollscheid B. (2018). HATRIC-based identification of receptors for orphan ligands. Nature Communications, 9, 1519.

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Cell Culture Conditions for Lymphoma Lines

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HBL-1, TMD8, OCI-LY7 and SU-DHL-16 cell lines were gifts from Dr. Fu, University of Nebraska Medical Center (Omaha, NE, USA). SU-DHL-2, SU-DHL-6, Pfeiffer, OCI-LY10, OCI-LY8, KARPAS-422 and U2932 cell lines were obtained from ATCC (Manassas, VA, USA) and DSMZ (Braunschweig, Germany). Cells were cultured in IMDM, DMEM or RPMI 1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10–20% fetal bovine serum (FBS; Gibco, Life Technology) and penicillin-streptomycin in a humidified atmosphere of 5% CO₂ at 37 °C.

Wang X., Wang D., Ding N., Mi L., Yu H., Wu M., Feng F., Hu L., Zhang Y., Zhong C., Ye Y., Li J., Fang W., Shi Y., Deng L., Ying Z., Song Y, & Zhu J. (2021). The Synergistic Anti-Tumor Activity of EZH2 Inhibitor SHR2554 and HDAC Inhibitor Chidamide through ORC1 Reduction of DNA Replication Process in Diffuse Large B Cell Lymphoma. Cancers, 13(17), 4249.

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CTLA-4 Modulation in DLBCL Cell Line

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DLBCL cell line U2932 (ATCC, USA) was cultured in RPMI1640 medium containing 10% fetal bovine serum (Hyclone, USA). After washing with PBS three times, CTLA-4 plasmid and CTLA-4 siRNA (designed and synthesized by Shanghai Genechem Company, China) were transfected into U2932 using Lipofectamine 3000 (Thermo Fisher Scientific, USA). After 72 h of culture, U2932 was collected in each group. Expression of CTLA-4 was detected by flow cytometry, and TGF-β level was detected by ELISA.

Chen Y., Li M., Cao J., Cai G., Li X., Liu Y, & Chen W. (2021). CTLA-4 promotes lymphoma progression through tumor stem cell enrichment and immunosuppression. Open Life Sciences, 16(1), 909-919.

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Silencing TUC338 in DLBCL cell lines

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Two DLBCL cell lines U2932 and OCI-Ly3 were purchased from ATCC and cultured in DMEM medium supplemented with 10% FBS. Cell transfection was conducted by using Lipofectamine 2000 (Invitrogen, CA, USA). For constructing the cell lines stably silencing TUC338, two validated shRNAs targeting TUC338 were inserted into psi-LVRU6GP lentiviral vector (GeneCopoeia, CA, USA), followed by transfection into U2932 and OCI-Ly3 cells. After 48 h, puromycin was added to culture medium to screen stable cell lines.

Li Y., Jia Z., Zhao H., Liu X., Luo J., Cui G, & Kong X. (2021). TUC338 Promotes Diffuse Large B Cell Lymphoma Growth via Regulating EGFR/PI3K/AKT Signaling Pathway. Journal of Oncology, 2021, 5593720.

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DLBCL Cell Line Culturing Protocol

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The human DLBCL cell lines U2932, OCI-LY10, SUDHL2, WSU-DLCL2, and DB were originally obtained from the American Type Culture Collection (Manassas, VA, USA) and grown in IMDM medium. HEK293T cells were cultured in DMEM medium. All cells were cultured with 10% fetal bovine serum at 37 °C in a humidified atmosphere consisting of 5% CO₂.

Xu L., Jiao J., Sun X., Sang W., Gao X., Yang P., Yan D., Song X., Sun C., Liu M., Qin Y., Tian Y., Zhu F., Zeng L., Li Z, & Xu K. (2020). Cladribine Induces ATF4 Mediated Apoptosis and Synergizes with SAHA in Diffuse Large B-Cell Lymphoma Cells. International Journal of Medical Sciences, 17(10), 1375-1384.

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Culturing 16 DLBCL Cell Lines

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The 16 DLBCL cell lines (DOHH2, HT, OCI-LY19, DB, OCI-LY1, SUDHL-4, SUDHL-5, SUDHL-10, NUDHL-1, OCI-LY7, WSU-DLCL2, SUDHL-6, NUDUL-1, U2932, OCI-LY3, and RI-1) were purchased from the American Type Culture Collection or from DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany). They were grown in RPMI-1640 Glutamax medium (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 10% fetal bovine serum (FBS) (PAA laboratory GmbH, Pasching, Austria) (U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, and WSU-DLCL2 cells), 20% FBS (OCI-LY3, DB, SUDHL-5, NUDHL-1, and SUDHL-6 cells), or 15% FBS (NUDUL-1 cells). OCI-LY1, and OCI-LY7 cells were cultured in IMDM Glutamax (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 20% FBS, and OCI-LY19 cells in MEM alpha modified Glutamax (Gibco, Invitrogen, Cergy Pontoise, France) with 20% FBS. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO₂. Contamination by Mycoplasma species was regularly monitored.

Devin J., Kassambara A., Bruyer A., Moreaux J, & Bret C. (2019). Phenotypic Characterization of Diffuse Large B-Cell Lymphoma Cells and Prognostic Impact. Journal of Clinical Medicine, 8(7), 1074.

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DLBCL Cell Lines Culture and Compound Reconstitution

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The human DLBCL cell lines BJAB, U2932, OCI-Ly3 were obtained from DSMZ, SUDHL-4, and SUDHL-6 were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). All cell lines were cultured in RPMI 1640 medium supplemented with 20% FBS and 100 U/ml penicillin/streptomycin (Gibco). OCI-Ly10 was purchased from Cobioer Biosciences Co., LTD (Nanjing, China) and cultured in IMDM with 20% FBS, 100 U/ml penicillin/streptomycin (Gibco) and 50 μM β-mercaptoethanol (Sigma-Aldrich). All cell lines were cultured at 37°C in a humidified atmosphere of 5% CO₂.
z-VRPR-fmk (Enzo Life Sciences) was dissolved in ddH₂O at a concentration of 50 μM throughout all experiments. MI-2, BPTES, QNZ, CPI-613 (Selleck), and PMA/Iono (Sigma-Aldrich) were reconstituted in DMSO (final DMSO concentration 0.1%) and their final concentrations were 1 μM, 2 μM, 5 μM, 100 μM, and 50/500 ng/ml, respectively.

Xia X., Zhou W., Guo C., Fu Z., Zhu L., Li P., Xu Y., Zheng L., Zhang H., Shan C, & Gao Y. (2018). Glutaminolysis Mediated by MALT1 Protease Activity Facilitates PD-L1 Expression on ABC-DLBCL Cells and Contributes to Their Immune Evasion. Frontiers in Oncology, 8, 632.

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Cell Lines Acquisition and Culture

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OCI-LY3 and Ramos were purchased from Cobioer Biotechnology Co., Ltd (Nanjing, China); OCI-LY10 was from Guandao Bioengineering Co., Ltd (Shanghai, China); U2932, Raji, Rec-1, Jeko-1, Maver-1 and JM1 were purchased from the American Type Culture Collection (ATCC). OCI-LY3 and Raji cells were cultured in IMDM (Gibco, US), HEK293T cells were cultured in DMEM (Gibco, US), the rest of cell lines were cultured in RPMI 1640 (Gibco, US). All mediums were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution.

Li S., He X., Gan Y., Zhang J., Gao F., Lin L., Qiu X., Yu T., Zhang X., Chen P., Tong J., Qian W, & Xu Y. (2021). Targeting miR-21 with NL101 blocks c-Myc/Mxd1 loop and inhibits the growth of B cell lymphoma. Theranostics, 11(7), 3439-3451.

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Cell Lines for B-Cell Lymphoma Research

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Diffuse Large B-Cell lines DHL-6, DHL-4, DHL-10, Karpas-422, RL, TMD-8, U2932, and RIVA; DHL cell lines ROS-50, DOHH-2 and VAL; and BL cell lines Ramos, Daudi and Raji were obtained from American Type Culture Collection (Rockville, MD). The rituximab resistant cell lines, RL 4RH, U2932 4RH and Raji 4RH was developed in the Hernandez Laboratory (27) . OCI-LY1 was a gift from the Lou Staudt Lab. All cell lines were maintained in RPMI 1640 (Thermo Fisher, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Atlanta Biologicals, Norcross, GA), 5mM HEPES, 100U/mL penicillin and 100μg/mL streptomycin (Thermo Fisher, Grand Island, NY) at 37°C and 5%CO 2 .

Torka P., Russell T., Mavis C., Gu J., Ghione P., Barth M, & Hernandez-Ilizaliturri F.J. (2023). AMG176, an MCL-1 inhibitor, is active in pre-clinical models of aggressive B-cell lymphomas. Leukemia & lymphoma, 64(6).

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