In this study, cell lines QGY-7703, Hela and HL-60 were purchased from Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). Cells were cultured in 10% fetal-bovine-serum-containing Dulbecco’s Modified Eagle’s medium or serum-free medium (Gibco, Grand Island, NY, USA) at 37°C in humidified air with 5% CO2.
QGY-7703 and HL-60 cells were cocultured using a transwell chamber culture system containing 0.4 µm pore polycarbonate membrane inserts (Corning, Corning, NY, USA). HL-60 cells were cultured in the upper chamber of the transwell culture system and QGY-7703 cells were cultured in the lower chamber. In this system, both cell lines shared the same culture media.
QGY-7703 and HL-60 cells were cocultured using a transwell chamber culture system containing 0.4 µm pore polycarbonate membrane inserts (Corning, Corning, NY, USA). HL-60 cells were cultured in the upper chamber of the transwell culture system and QGY-7703 cells were cultured in the lower chamber. In this system, both cell lines shared the same culture media.