MTT was purchased from Sigma‐Aldrich (USA). Chloroquine diphosphate, E‐64, PKI‐402 and rapamycin were purchased from MedChemExpress (USA). The primary antibodies against LC3B, SQSTM1/p62, TFEB, PINK1, parkin, Fis1, Drp1, Mfn1 and Mfn2 were purchased from Abcam (USA), and LAMP1, beta‐actin, alpha‐tubulin, cathepsin B, cathepsin D and cytochrome c were purchased from Proteintech (USA).
Primary antibodies against lc3b
Manufactured by Abcam
Sourced in United States
Primary antibodies against LC3B are immunoglobulin molecules that specifically recognize and bind to the LC3B protein, a key component of the autophagy process. These antibodies can be used to detect and quantify the expression levels of LC3B in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Sqstm1 p62, by Abcam (1 mentions)
Parkin, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Cathepsin d, by Proteintech (1 mentions)
Cytochrome c, by Proteintech (1 mentions)
Pink1, by Abcam (1 mentions)
Chloroquine diphosphate, by MedChemExpress (1 mentions)
Pki 402, by MedChemExpress (1 mentions)
Lamp1, by Proteintech (1 mentions)
Cathepsin b, by Proteintech (1 mentions)
Alpha tubulin, by Proteintech (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Rapamycin, by MedChemExpress (1 mentions)
Fitc conjugated secondary antibody, by Abcam (1 mentions)
2 protocols using primary antibodies against lc3b
1
Autophagy and Mitochondrial Dynamics Assay
2
Immunofluorescence Staining of LC3B
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and then blocked with 1% bovine serum albumin (BSA). The cells were then incubated with primary antibodies against LC3B (Abcam, USA) overnight at 4 °C. After washing with PBS, the cells were incubated with FITC-conjugated secondary antibodies (Abcam, USA) for 0.5 hours, stained with DAPI for 2 minutes, and then observed by confocal fluorescence microscopy.
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