Log In Sign up
The largest database of trusted experimental protocols

Endofree plasmid purification kit

Manufactured by Macherey-Nagel
Visit Supplier

The EndoFree plasmid purification kit is a laboratory product designed for the isolation and purification of plasmid DNA from bacterial cultures. It provides a reliable and efficient method for removing endotoxins, ensuring the obtained plasmid DNA is suitable for sensitive downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Fast green, by Merck Group (2 mentions) Swiss mice, by Janvier Labs (2 mentions)

2 protocols using endofree plasmid purification kit

1

In utero electroporation of mouse embryos

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
In utero electroporation were performed as described previously13 (link),40 (link) using Swiss mice (Janvier). Animal experimentations were performed at the IGBMC animal facilities. The study has the Animal Experimentation Research Ethics Committee approval (N° 2014-059). Briefly, timed pregnant mice (E14.5) were anaesthetized with isoflurane (2 l per min of oxygen, 4% isoflurane during sleep and 2% isoflurane during surgery operation; Minerve). The uterine horns were exposed and a lateral ventricle of each embryo was injected using pulled glass capillaries with Fast Green (2 μg/ml; Sigma) combined with a final concentration of 1µg/µl of DNA constructs prepared by EndoFree plasmid purification kit (Macherey Nagel). The expression vector pCAGGS-Tomato was systematically co-electroporated and the fluorescent Tomato protein was used to visualize electroporated cells. Plasmids were further electroporated into the neuronal progenitors adjacent to the ventricle by delivering five electric pulses at 50V for 50ms at 950ms intervals using a CUY21EDIT electroporator (Sonidel Ltd). After electroporation, embryos were placed back in the abdominal cavity and development was allowed to continue until E16, E18 or P2. Embryos or pups brains were dissected and fixed in 4% PFA in PBS overnight.
Broix L., Jagline H., Ivanova E., Schmucker S., Drouot N., Clayton-Smith J., Pagnamenta A.T., Metcalfe K.A., Isidor B., Louvier U.W., Poduri A., Taylor J.C., Tilly P., Poirier K., Saillour Y., Lebrun N., Stemmelen T., Rudolf G., Muraca G., Saintpierre B., Elmorjani A., Moïse M., Weirauch N.B., Guerrini R., Boland A., Olaso R., Masson C., Tripathy R., Keays D., Beldjord C., Nguyen L., Godin J., Kini U., Nischké P., Deleuze J.F., Bahi-Buisson N., Sumara I., Hinckelmann M.V, & Chelly J. (2016). Mutations in the HECT domain of NEDD4L lead to AKT/mTOR pathway deregulation and cause periventricular nodular heterotopia. Nature genetics, 48(11), 1349-1358.
+ Open protocol
+ Expand
2

In utero electroporation of mouse embryos

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
In utero electroporation were performed as described previously13 (link),40 (link) using Swiss mice (Janvier). Animal experimentations were performed at the IGBMC animal facilities. The study has the Animal Experimentation Research Ethics Committee approval (N° 2014-059). Briefly, timed pregnant mice (E14.5) were anaesthetized with isoflurane (2 l per min of oxygen, 4% isoflurane during sleep and 2% isoflurane during surgery operation; Minerve). The uterine horns were exposed and a lateral ventricle of each embryo was injected using pulled glass capillaries with Fast Green (2 μg/ml; Sigma) combined with a final concentration of 1µg/µl of DNA constructs prepared by EndoFree plasmid purification kit (Macherey Nagel). The expression vector pCAGGS-Tomato was systematically co-electroporated and the fluorescent Tomato protein was used to visualize electroporated cells. Plasmids were further electroporated into the neuronal progenitors adjacent to the ventricle by delivering five electric pulses at 50V for 50ms at 950ms intervals using a CUY21EDIT electroporator (Sonidel Ltd). After electroporation, embryos were placed back in the abdominal cavity and development was allowed to continue until E16, E18 or P2. Embryos or pups brains were dissected and fixed in 4% PFA in PBS overnight.
Broix L., Jagline H., Ivanova E., Schmucker S., Drouot N., Clayton-Smith J., Pagnamenta A.T., Metcalfe K.A., Isidor B., Louvier U.W., Poduri A., Taylor J.C., Tilly P., Poirier K., Saillour Y., Lebrun N., Stemmelen T., Rudolf G., Muraca G., Saintpierre B., Elmorjani A., Moïse M., Weirauch N.B., Guerrini R., Boland A., Olaso R., Masson C., Tripathy R., Keays D., Beldjord C., Nguyen L., Godin J., Kini U., Nischké P., Deleuze J.F., Bahi-Buisson N., Sumara I., Hinckelmann M.V, & Chelly J. (2016). Mutations in the HECT domain of NEDD4L lead to AKT/mTOR pathway deregulation and cause periventricular nodular heterotopia. Nature genetics, 48(11), 1349-1358.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!