S r r 3 1 3 4 dichlorophenyl ethyl amino 2 hydroxypropyl cyclohexylmethyl phosphinic acid cgp 54626

Drugs used included GABA, hexamethonium bromide (both from Sigma Aldrich, Castle Hill, New South Wales, Australia), Bicuculline, [S-(R^*,R^*)]-[3-[[1-(3,4-Dichlorophenyl)ethyl]amino]-2-hydroxypropyl](cyclohexylmethyl) phosphinic acid (CGP 54626) and (1,2,5,6-Tetrahydropyridin-4-yl)methylphosphinic acid (TPMPA) (all from Tocris Bioscience, Avonmouth Bristol UK). All drugs were diluted in distilled water to make stock solutions and then again in physiological saline to their working concentration on the day of experimentation.

Methyl sulfoxide (DMSO), PTX, K-gluconate, Lucifer yellow, K₂-ATP, Na₃-GTP, 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (Na-TES), nicotinamide adenine dinucleotide phosphate (NADP⁺), glutamate dehydrogenase, sodium dodecyl sulfate (SDS), ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), Triton X-100, and 0.01 M PBS were purchased from Sigma. [S-(R*,R*)]-[3-[[1-(3,4-Dichlorophenyl)ethyl]amino]-2-hydroxypropyl] (cyclohexylmet-hyl) phosphinic acid (CGP54626) and (3-aminopropyl) ethylphosphinic acid hydrochloride (CGP36216) were purchased from Tocris. Morphine was from Shenyang No. 1 Pharmaceutical Factory, China. Percoll was purchased from Amersham Biosciences Corporation. Other AR grade reagents were from Shanghai Chemical Plant. PTX or CGP54626 was dissolved in DMSO and others were dissolved in ddH₂O. When DMSO was used as the vehicle, drugs were initially dissolved in 100% DMSO and then diluted into ASFC at a final DMSO concentration of less than 0.5%, which had no detectable effects on the parameters we observed.