Rabbit polyclonal antibody anti-NBCn2-Ct directed against the PDZ-Ct of NBCn2 was generated by GenScript (Nanjing, China) with a synthetic peptide “IESRKEKKADSGKGVDRETC”. A cysteine was introduced at the Ct end for conjugation to keyhole limpet hemocyanin. The antibody was affinity-purified with the immunogen. Anti-α1 (directed against the α1 subunit of Na+-K+-ATPase) was purchased from Cell Signaling Technology (cat#3010, Danvers, MA, USA). Rabbit anti-Myc (cat#AE009), mouse anti-HA (cat#AE008) were purchased from Abclonal (Cambridge, MA, USA). Normal rabbit IgG (cat#sc-2027) and normal mouse IgG (cat#sc-2025) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Goat-anti-rabbit secondary antibody conjugated with horse radish peroxidase (HRP) was purchased from Thermo Scientific (Rockford, IL, USA). Goat-anti-mouse secondary antibody conjugated with HRP was purchased from Beyotime (cat#A0216, Beyotime, Haimen, China). Alexa 488 goat-anti-mouse (cat#705-545-003) from Jackson ImmunoResearch (West Grove, PA, USA) and Dylight 549 goat-anti-rabbit (cat#E032320-01) secondary antibodies were purchased from EarthOx (Millbrae, CA, USA).
Rabbit anti myc
Manufactured by ABclonal
Sourced in United States
Rabbit anti-Myc is a primary antibody that recognizes the c-Myc protein. It is a useful tool for detecting and studying the expression and localization of c-Myc in various experimental systems.
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Lab products found in correlation
2 protocols using rabbit anti myc
1
Antibody Generation and Characterization
2
Immunoprecipitation of ApoD and RABV G
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HEK-293T cells were co-transfected with the mammalian expression vector pcDNA3.0-ApoD -MYC (for ApoD expression) and/or PCAGGS-G -FLAG (pC-rRC-HL-G -FLAG and pC-GX074-G - FLAG ) (for RABV G protein expression). At 24 h post-transfection (hpt), the cells were washed with cold phosphate-buffered saline (PBS) and lysed with NP-40 lysis buffer containing an anti-protease cocktail (100× protease inhibitor cocktail) for 40 min at 4°C. The cell lysates were centrifuged at 12,000 g and 4°C for 10 min. The supernatant was transferred to a new tube and incubated with mouse anti-FLAG (Abmart, Shanghai, China, M20008, 1:100) or rabbit anti-MYC (ABclonal, Wuhan, China, AE070, 1:500) monoclonal antibodies for 8 h. Next, the samples were incubated with the protein A/G agarose (Beyotime, Shanghai, China, P2055) for 8 h at 4°C with rotation. The agarose beads were washed five times with cold PBS, and the bound proteins were examined using Western blotting analysis.
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