γ1 actin 2f3

Manufactured by Fujifilm

Sourced in United States

γ1-actin (2F3;) is a laboratory reagent used in biological research. It is an antibody that specifically binds to the gamma-1 isoform of the actin protein, which is a structural component of the cytoskeleton in eukaryotic cells. This product can be used in various techniques, such as immunohistochemistry and Western blotting, to detect and quantify the presence of γ1-actin in cell and tissue samples.

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Lab products found in correlation

Alexa 488 conjugated anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions) Ac 15, by Merck Group (1 mentions) Ab92573, by Abcam (1 mentions) Peroxidase conjugated anti mouse igg h l antibody, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Vsv g p5d4, by Santa Cruz Biotechnology (1 mentions) β actin ac 15, by Merck Group (1 mentions) Goat anti rat igg h l, by Jackson ImmunoResearch (1 mentions) Peroxidase conjugated goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions) Cytochrome c 7h8.2c12, by BD (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase linked secondary antibody, by Jackson ImmunoResearch (1 mentions) Imagequant las 4000 mini, by GE Healthcare (1 mentions) Tradd 37 tradd, by BD (1 mentions) Flag 1e6, by Fujifilm (1 mentions) Tnf r1 h 5, by Santa Cruz Biotechnology (1 mentions) Rela c 20, by Santa Cruz Biotechnology (1 mentions) Las 4000, by Cytiva (1 mentions)

3 protocols using γ1 actin 2f3

Immunoblotting of Cytoskeletal Proteins

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Primary antibodies for β-actin (AC-15; Sigma-Aldrich), γ1-actin (2F3; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), calnexin (ab92573; Abcam, Cambridge, UK), ICAM-1 (15.2; Leinco Technologies, Inc., St. Louis, MO, USA), ICAM-1 (28; BD Biosciences, San Diego, CA, USA), peroxidase-conjugated anti-mouse IgG(H+L) antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), Alexa^® 488-conjugated anti-mouse IgG antibody (Thermo Fisher Scientific), and Alexa^® 594-conjugated anti-rabbit IgG antibody (Thermo Fisher Scientific) as secondary antibodies were used in this study.

Nakano K., Sasaki S, & Kataoka T. (2022). Bioactive Evaluation of Ursane-Type Pentacyclic Triterpenoids: β-Boswellic Acid Interferes with the Glycosylation and Transport of Intercellular Adhesion Molecule-1 in Human Lung Adenocarcinoma A549 Cells. Molecules, 27(10), 3073.

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Antibody Immunoblotting Protocol

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Mouse monoclonal antibodies to FLAG (IE6; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), VSV-G (P5D4; Santa Cruz Biotechnology, Dallas, TX, USA), the VSV glycoprotein (P5D4, SAB4200695; Sigma-Aldrich), GRP75 (D-9; Santa Cruz Biotechnology), cytochrome c (7H8.2C12; BD Biosciences, Franklin Lakes, NJ, USA), PARP-1 (C2-10; R &D Systems, Minneapolis, MN, USA), γ1-actin (2F3; FUJIFILM Wako Pure Chemical Corporation), β-actin (AC-15; Sigma-Aldrich), and GAPDH (6C5; Santa Cruz Biotechnology), a rat monoclonal antibody to caspase-7 (11E4; Sigma-Aldrich), and a rabbit polyclonal antibody to HSP60 (insect, ADI-SPA-805; Enzo Life Sciences, Farmingdale, NY, USA) were used as primary antibodies. Peroxidase-conjugated goat anti-mouse IgG (H + L) (115-035-146; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), goat anti-rabbit IgG (H + L) (111-035-144; Jackson ImmunoResearch Laboratories), and goat anti-rat IgG (H + L) (J112-035-143; Jackson ImmunoResearch Laboratories) were used as secondary antibodies.

Xu J., Hashino T., Tanaka R., Kawaguchi K., Yoshida H, & Kataoka T. (2023). The BCL-2 family protein BCL-RAMBO interacts and cooperates with GRP75 to promote its apoptosis signaling pathway. Scientific Reports, 13, 14041.

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Western Blot Analysis of Signaling Proteins

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The preparation of cell lysates and western blotting were performed as described previously. 16, 17 Protein samples (30 μg) in sample buffer (62.5 mM Tris, 2% SDS, 10% glycerol, 0.003% bromophenol blue and 288 mM 2-mercaptoethanol) were boiled at 100 °C for 5 min, fractionated by SDS-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes. The membranes were incubated with primary antibodies specific to γ1-actin (2F3; Wako Pure Chemical Industries), FLAG (1E6; Wako Pure Chemical Industries), IκBα (clone 25; BD Biosciences, San Jose, CA, USA), IκB kinase β (D30C6; Cell Signaling Technology, Danvers, MA, USA), RelA (C-20; Santa Cruz Biotechnology, Santa Cruz, CA, USA), RIP1 (38/RIP; BD Biosciences), TNF-R1 (H-5; Santa Cruz Biotechnology), TRADD (37/ TRADD; BD Biosciences), TRAF2 (C-20; Santa Cruz Biotechnology) and horseradish peroxidase-linked secondary antibodies (Jackson ImmunoResearch Labora-tories, West Grove, PA, USA). Blots were analyzed by ImageQuant LAS 4000 mini (GE Healthcare).

Quach H.T., Tanigaki R., Yokoigawa J., Yamada Y., Niwa M., Hirano S., Shiono Y., Kimura K.I, & Kataoka T. (2017). Allantopyrone A interferes with multiple components of the TNF receptor 1 complex and blocks RIP1 modifications in the TNF-α-induced signaling pathway. The Journal of antibiotics, 70(9).

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