Anti mouse irdye 680cw

Manufactured by LI COR

The Anti-mouse IRDye 680CW is a fluorescent dye that can be used to label antibodies or other proteins for detection in infrared-based imaging applications. It has an excitation maximum at 682 nm and an emission maximum at 705 nm, making it suitable for use with near-infrared imaging systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit irdye 800cw, by LI COR (4 mentions) Fugene hd, by Promega (3 mentions) Anti mouse irdye 800cw, by LI COR (3 mentions) Flag m2 monoclonal ab, by Merck Group (3 mentions) Irdye 680cw anti rabbit, by LI COR (3 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Anti rabbit irdye 800lt, by LI COR (2 mentions) Tmtsixplex isobaric label reagent, by Thermo Fisher Scientific (2 mentions) Proteinase k, by Thermo Fisher Scientific (2 mentions) Sequencing grade modified trypsin, by Promega (2 mentions) Mtmr1, by Abcam (2 mentions) D2hgdh, by Abcam (2 mentions) Igbp1, by Cell Signaling Technology (2 mentions) Smad9, by Abcam (2 mentions) Actr5, by Proteintech (2 mentions) Akap8, by Abcam (2 mentions) Gstp1, by Cell Signaling Technology (2 mentions) Me bs, by Merck Group (2 mentions) Val boropro, by Merck Group (2 mentions) Bortezomib bort, by Merck Group (2 mentions)

Close spelling variants of this product

IRDye 680CW (55 citations) IRDye 680CW goat anti-mouse IgG (21 citations) IRDye 680CW goat anti-mouse (16 citations) IRDye 680CW donkey anti-mouse IgG (9 citations) IRDye® 680CW Goat anti-Mouse antibody (4 citations) Donkey anti-mouse IRDye 680CW (4 citations) IRDye® 680CW goat anti-mouse IgG (H + L; (3 citations) Anti-mouse IgG IRDye 680CW (2 citations) IRDye 680CW goat anti-mouse IgG secondary antibody (2 citations)

7 protocols using anti mouse irdye 680cw

Immunoblotting of Purified Eluents

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting, 10% of the total eluent, purified for mass spectrometry, was loaded onto polyacrylamide gels. SDS–PAGE and immunoblotting were performed following standard procedures. The antibodies or fluorescent labels used were as follows: anti-myc 9E10 (Abcam); IRDye 800CW streptavidin (LI-COR); anti-mouse IRDye 680CW (LI-COR).

Remnant L., Booth D.G., Vargiu G., Spanos C., Kerr A.R, & Earnshaw W.C. (2019). In vitro BioID: mapping the CENP-A microenvironment with high temporal and spatial resolution. Molecular Biology of the Cell, 30(11), 1314-1325.

+ Open protocol

+ Expand

Immunoblotting Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-Centaurin alpha1 G-4 (ADAP1) (Santa Cruz, sc-390498) (1:1000); Anti-p65/NF-κB (Santa Cruz, sc-372) (1:4000); Anti-phos-ERK1/2 Thr202/Tyr204 (Cell Signaling Technology, 4370) (1:1000); Anti-ERK1/2 (Cell Signaling Technology, 4696 or 4695) (1:2000); Anti-Fos (Cell Signaling Technology, 2250) (1:1000); Anti-Jun (Cell Signaling Technology, 9165) (1:1000); Anti-Flag M2 (Sigma, F31165) (1:10,000); Anti-PKCθ (Cell Signaling Technology, 13643) (1:5000); Anti-CD3ε (Cell Signaling Technology, 4443) (1:5000); Anti-GAPDH (Cell Signaling Technology, 2118) (1:5000); hFAB Rhodamine anti-Actin (Bio-Rad, 12004166); (1:10,000); Anti-StrepTactin-HRP (Bio-Rad, 161-0381) (1:10,000); Anti-rabbit IRDye 800CW (Licor, 926-32211) (1:10,000); Anti-mouse IRDye 680CW (Licor, 925-68072) (1:10,000); Anti-mouse HRP (Cell Signaling Technology, 7076) (1:10,000); Anti-rabbit HRP (Cell Signaling Technology, 7074), (1:10,000); Anti-rat HRP (Abcam, ab97057) (1:10,000).

Ramirez N.G., Lee J., Zheng Y., Li L., Dennis B., Chen D., Challa A., Planelles V., Westover K.D., Alto N.M, & D’Orso I. (2022). ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS–ERK–AP-1 T cell signaling-transcriptional axis. Nature Communications, 13, 1109.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Expression

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein extracts were transferred to polyvinylidene difluoride membranes as previously described (3 (link)). Membranes were blocked with Odyssey Blocking Buffer in tris-buffered saline (LI-COR) and incubated overnight at 4°C with the following primary antibodies: Hspa5 (1:2000; Proteintech, 11587-1-AP), Pdia6 (1:2000; Abcam, ab11432), Biotin Ligase Epitope Tag (for Tavi-tag detection, 1:1000; Abcam, ab106159), PSD95 (1:5000; Thermo Fisher Scientific, 6G6-1C9), hemagglutinin (HA) (1:2000; Millipore Sigma), phospho-tau AT8 (1:5000; BioLegend, 806503), actin (1:10,000; Thermo Fisher Scientific), ATF4 (1:500; Thermo Fisher Scientific, PA5-27576), ATF6 (1:500; Thermo Fisher Scientific, PA5-114886), and phospho-IRE1α (1:500; Thermo Fisher Scientific, PA5-85738). Membranes were washed and incubated with appropriate IRDye immunoglobulin G (IgG) secondary antibodies, including anti-rabbit IRDye 800LT (1:5000; LI-COR) and anti-mouse IRDye 680CW (LI-COR). Images were acquired using the Odyssey Infrared Imaging System (LI-COR). Quantification of Western blot bands was performed using Image Studio Lite v.5.2 (LI-COR).

Chatterjee S., Bahl E., Mukherjee U., Walsh E.N., Shetty M.S., Yan A.L., Vanrobaeys Y., Lederman J.D., Giese K.P., Michaelson J, & Abel T. (2022). Endoplasmic reticulum chaperone genes encode effectors of long-term memory. Science Advances, 8(12), eabm6063.

+ Open protocol

+ Expand

Quantification of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-cell lysates were run on a 4–20% Tris-HCl Protein Gel (BIO-RAD, # 3450033) and transferred to methanol-activated polyvinylidene difluoride membranes. Membranes were blocked with Odyssey® Blocking Buffer in TBS (LI-COR) and incubated overnight at 4 °C with the following primary antibodies: pan-HA (1:1000, Cell signaling), YFP (1:1000, Abcam), and Actin (1:10,000, ThermoFisher Scientific). Membranes were washed and incubated with anti-rabbit IRDye 800LT (1:5000, LI-COR, # 926-32211) and anti-mouse IRDye 680CW (1:5000, LI-COR, #926-68022). Images were acquired using the Odyssey Infrared Imaging System (LI-COR). Quantification of western blot bands was performed using Image Studio Lite ver5.2 (LI-COR).

Vanrobaeys Y., Mukherjee U., Langmack L., Beyer S.E., Bahl E., Lin L.C., Michaelson J.J., Abel T, & Chatterjee S. (2023). Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation. Nature Communications, 14, 6100.

+ Open protocol

+ Expand

Antibody-Mediated Protein Interactome Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), GSDMD Rabbit monoclonal Ab (Abcam, Ab209845), FLAG® M2 monoclonal Ab (Sigma, F3165), CARD8 C terminus Rabbit polyclonal Ab (Abcam, Ab24186), CARD8 N-terminal Rabbit polyclonal Ab (Abcam, Ab194585), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), MTMR1 (Abcam, Ab 240569), D2HGDH (Abcam, Ab233516), ATF-3 (Cell Signaling Tech, D2Y5W), IGBP1 (Cell Signaling Tech, 5F6), SMAD9 (Abcam, Ab124094), ARF1 (Abcam, Ab58578), ACTR5 (Proteintech, 21505), AKAP8 (Abcam, Ab72196), GSTP1 (Cell Signaling Tech, 3F2), SET (Abcam, Ab1183), NLRP1B (Vance laboratory, 2A12), IRDye 800CW anti-rabbit (LICOR, 925-32211), IRDye 800CW anti-mouse (LI-COR, 925-32210), IRDye 680CW anti-rabbit (LI-COR, 925-68073), IRDye 680CW anti-mouse (LI-COR, 925-68072). Other reagents used include: Val-boroPro (VbP) (Okondo et al., 2017 (link)), Bortezomib (Bort; MilliporeSigma, 504314), MLN4924 (Cayman, 15217), bestatin methyl ester (Me-Bs; Sigma, 200485), sequencing grade modified trypsin (Promega, V5113), proteinase K (PROK; Invitrogen, 25530049), FuGENE HD (Promega, E2311), TMTsixplex Isobaric Label Reagents (ThermoFisher Scientific).

Chui A.J., Griswold A.R., Taabazuing C.Y., Orth E.L., Gai K., Rao S.D., Ball D.P., Hsiao J.C, & Bachovchin D.A. (2020). Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell reports, 33(2), 108264.

+ Open protocol

+ Expand

Antibody-Mediated Protein Interactome Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Inflammasome Activation and Pyroptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2–33422), FLAG® M2 monoclonal Ab (Sigma, F3165), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), CASP1 p20 Rabbit polyclonal Ab (Cell Signaling Tech, 2225s), CASP1 p12/10 Rabbit monoclonal Ab (Abcam, ab179515), CASP4 Rabbit polyclonal Ab (Cell Signaling Tech, 4450S), CASP5 Rabbit monoclonal Ab (Cell Signaling Tech, 46680S), Myc Mouse monoclonal Ab (Cell Signaling Tech, 2276S), V5 Rabbit monoclonal Ab (Cell Signaling Tech, 13202S), hIL-1β Goat Polyclonal Ab (R&D systems, AF-201-NA), hIL-18 Goat Ab (R&D systems, af2548), hIL-1α Recombinant Ab (PeproTech, 200–01A), PARP Rabbit polyclonal Ab (Cell Signaling Tech, 9542S), HA Rabbit monoclonal Ab (Cell Signaling Tech, 3724S), mIL-1β Goat polyclonal Ab (R&D systems, AF-401-NA), CASP11 Rat monoclonal Ab (Novus Biologicals, NB120–10454), FKBP12 Rabbit polyclonal Ab (Abcam, ab24373). IRDye 800CW anti-rabbit (LICOR, 925–32211), IRDye 800CW anti-mouse (LI-COR, 925–32210), IRDye 680CW anti-rabbit (LI COR, 925–68073), IRDye 680CW anti-mouse (LI-COR, 925–68072). Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50–101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris™ 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79).

Exconde P.M., Hernandez-Chavez C., Bray M.B., Lopez J.L., Srivastava T., Egan M.S., Zhang J., Shin S., Discher B.M, & Taabazuing C.Y. (2023). The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspases. bioRxiv.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!