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Sybr green quantitative real time pcr

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SYBR Green is a fluorescent dye used for quantitative real-time PCR. It binds to double-stranded DNA and emits a fluorescent signal that can be detected and measured during the PCR process to quantify the amount of target DNA present in a sample.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (1 mentions) Real time pcr system, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SYBR Green (3216 citations) Quantitative real-time PCR (33 citations) SYBR Green real-time PCR (21 citations) SYBR green-based quantitative real-time PCR (4 citations) SYBR green real-time quantitative reverse transcription PCR (qRT-PCR) (3 citations) SYBR Green real-time quantitative RT-PCR reagent (2 citations) SYBR green-based real-time quantitative RT-PCR (2 citations) SYBR green-based real-time quantitative PCR assays (2 citations)

2 protocols using sybr green quantitative real time pcr

1

Quantitative Real-time PCR Analysis

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EphB1, p21, Bcl-2, and p53 mRNA expression levels were analyzed using SYBR Green quantitative real-time PCR (qRT-PCR; Applied Biosystems Applera) with HPRT as a reference gene. Complementary DNA synthesis was accomplished from 1 μg RNA (RNeasy mini kit; Qiagen). Relative mRNA expression levels of the gene of interest were calculated using the ΔΔCt method relatively to the mean HPRT mRNA expression levels. Primer sequences are depicted in Supplementary Table S1.
Kampen K.R., Scherpen F.J., Garcia-Manero G., Yang H., Kaspers G.J., Cloos J., Zwaan C.M., van den Heuvel-Eibrink M.M., Kornblau S.M, & De Bont E.S. (2015). EphB1 Suppression in Acute Myelogenous Leukemia: Regulating the DNA Damage Control System. Molecular cancer research : MCR, 13(6), 982-992.
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2

Quantitative Analysis of RNA Levels

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At 8 weeks after treatment, the mouse liver was obtained and subjected to total RNA extraction using Trizol reagent. Relative levels of amplificants were identified utilizing SYBR Green quantitative realtime pcr (qPCR) mix (applied biosystems) on Applied Biosystems Real-Time PCR System (Table 1).
Jiang L.S., Li W., Zhuang T.X., Yu J.J., Sun S., Ju Z.C., Wang Z.T., Ding L.L, & Yang L. (2021). Ginsenoside Ro Ameliorates High-Fat Diet-Induced Obesity and Insulin Resistance in Mice via Activation of the G Protein-Coupled Bile Acid Receptor 5 Pathway. The Journal of pharmacology and experimental therapeutics, 377(3).
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