Urolithin A was purchased from Cayman Chemical Company (#22,607). Bafilomycin-A1 (#B1793) and anti-p62/SQSTM1 antibody (#P0067) were purchased from Sigma-Aldrich. Anti-amyloid beta peptide antibody (MOAB-2) was purchased from Millipore (#MABN254). AT8 phospho-Tau antibody (Ser202, Thr205) was purchased from Thermo Fisher Scientific (#MN1020). Anti-β-amyloid antibody (6E10) (#803,017) was purchased from Biolegend. LC3 antibody was purchased from Proteintech (#14,600–1-AP). Actin antibody was purchased from Cell Signaling Technology (#3700) goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor™ 488, was purchased from Sigma-Aldrich (#A-11001). DMEM/F12 (#11,330,032) and DQ-BSA red (#D12051) were purchased from Thermo Fisher Scientific. Earl’s balanced salt solution (EBSS) (#24,010–043) was purchased from Gibco. DMEM (#10–013-CV), fetal bovine serum (#35–010-CV) and penicillin–streptomycin solution (#30–002-CI) were purchased from Corning. Beta amyloid oligomers were generated according to the manufacturer’s instructions using the beta amyloid (1–42), aggregation kit (#A-1170–2) purchased from rPeptide.
Anti β amyloid antibody 6e10
Manufactured by BioLegend
The Anti-β-amyloid antibody (6E10) is a lab equipment product that recognizes the N-terminus of the amyloid-beta (Aβ) peptide. It is a widely used tool in Alzheimer's disease research for the detection and analysis of Aβ.
Automatically generated - may contain errors
Lab products found in correlation
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Actin antibody, by Cell Signaling Technology (1 mentions)
Anti p62 sqstm1 antibody, by Merck Group (1 mentions)
Goat anti mouse igg h l, by Merck Group (1 mentions)
Dq red bsa, by Thermo Fisher Scientific (1 mentions)
Urolithin a, by Cayman Chemical (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
Alexa fluor 488, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Penicillin streptomycin solution, by Corning (1 mentions)
Lc3 antibody, by Proteintech (1 mentions)
Pannoramic 250, by 3DHISTECH (1 mentions)
2 protocols using anti β amyloid antibody 6e10
1
Amyloid β Oligomer Stimulation Protocol
2
Histological Analysis of Amyloid Plaques
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The left hemispheres of mice brains were fixed in 4% formalin solution overnight and then transferred to 30% saccharose solution. The 14 μm frozen sections were prepared and maintained at −20℃. The sections were stained with anti-β-amyloid antibody (6E10, Biolegend, 803002) overnight at 4℃. The FITC-labeled goat anti-mouse IgG (Zhongshan Golden Bridge, ZB2305) was used to report the first antibody. Finally, slices were sealed by mounting medium with DAPI (Zhongshan Golden Bridge, ZLI-9557). Slices were scanned with the fluorescencescanner (3D HISTECH, pannoramic 250) and the FITC marked plaques and cells were subsequently analyzed with Image J.
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