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Zombie violet live dead

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Zombie-Violet is a fluorescent dye used to differentiate between live and dead cells. It functions by selectively staining dead or dying cells, allowing for their identification in flow cytometry applications.

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Lab products found in correlation

Anti cd16 cd32, by BD (2 mentions) Facsymphony, by BD (1 mentions) Facsaria 2, by BD (1 mentions)

Close spelling variants of this product

Live/Dead Zombie Zombie Violet

3 protocols using zombie violet live dead

1

Comprehensive Immune Cell Staining

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Immune cells were stained for viability (Zombie-Violet live/dead) (Biolegend), FcR blocked (anti-CD16/CD32, BD Bioscience, Franklin lakes, USA), and stained with one of two panels (all Biolegend). Cocktail 1 for Macrophages: anti-mouse F4/80-PE, MHC-II/I-AE-PerCPCy5.5, Ly-6G-APC, CD11c-FITC. Cocktail 2 for NK cells: anti-mouse CD3-BV510, NKp46/CD335-PE-TexasRed, CD107a-PE, CD69-BV605, IFN-γ-PerCPCy5.5.
Kavian N., Hachim A., Poon L.L, & Valkenburg S.A. (2020). Vaccination with ADCC activating HA peptide epitopes provides 1 partial protection from influenza infection. Vaccine, 38(37), 5885-5890.
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2

Comprehensive Immune Cell Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immune cells were stained for viability (Zombie-Violet live/dead) (Biolegend), FcR blocked (anti-CD16/CD32, BD Bioscience, Franklin lakes, USA), and stained with one of two panels (all Biolegend). Cocktail 1 for Macrophages: anti-mouse F4/80-PE, MHC-II/I-AE-PerCPCy5.5, Ly-6G-APC, CD11c-FITC. Cocktail 2 for NK cells: anti-mouse CD3-BV510, NKp46/CD335-PE-TexasRed, CD107a-PE, CD69-BV605, IFN-γ-PerCPCy5.5.
Kavian N., Hachim A., Poon L.L, & Valkenburg S.A. (2020). Vaccination with ADCC activating HA peptide epitopes provides 1 partial protection from influenza infection. Vaccine, 38(37), 5885-5890.
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3

Multiparameter Flow Cytometry of Transduced Cells

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Cells were stained with Zombie violet Live/Dead (Biolegend) ahead of the primary stain with bispecific antibodies in PBS 2% FBS, whether they be from recombinant purification or mouse plasma. Cells were washed twice with PBS 2% FBS before an anti-his tag secondary stain (Jackson ImmunoResearch). Following an additional three washes, binding was assessed by flow cytometry using a BD FACSymphony (BD Biosciences). The sorting of transduced cells involved staining with a commercial anti-CA9 antibody (Invitrogen) before sorting on a BD FACSAria II (BD Biosciences). Sorting was conducted by The Wistar Institute Flow Cytometry Core.
O'Connell R.P., Liaw K., Wellhausen N., Chuckran C.A., Bhojnagarwala P.S., Bordoloi D., Park D., Shupin N., Kulp D., June C.H, & Weiner D. (2024). Format-tuning of in vivo-launched bispecific T cell engager enhances efficacy against renal cell carcinoma. Journal for immunotherapy of cancer, 12(6).
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