The siRNA against c-Jun (si-c-Jun) and control siRNA (si-C) were purchased from Santa Cruz Biotechnology [11 (link)]. Transfections were performed with 50 nM si-c-Jun, or 50 nM si-C using Effectene Transfection Reagents (Qiagen) according to the manufacturer’s protocol [15 (link)].
Control sirna si c
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Control siRNA (si-C) is a non-targeting small interfering RNA (siRNA) designed for use as a negative control in RNA interference (RNAi) experiments. It serves as a baseline to compare the effects of specific gene knockdown by experimental siRNAs.
Automatically generated - may contain errors
2 protocols using control sirna si c
1
Silencing c-Jun Gene Expression
2
siRNA-Mediated Silencing of GRP78 in Huh7 Cells
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Small interfering RNA (siRNA) against GRP78 (si-GRP78) and control siRNA (si-C) were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). These siRNA were validated in a previous study (4 ). These siRNAs (50 nM) were electroporated into Huh7 cells using the GenePulser Xcell system (Bio-Rad Laboratories, Hercules, CA, USA) at 850 µF and 220 V, according to the manufacturer's protocol (10 (link)).
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