Lncap

All animal experiments were performed under the guidelines of the Institute of Experimental Animal Sciences. The protocol was approved by the Animal Care and Use Committee of the Osaka University Graduate School of Medicine (approval 25-097-015). Male NOD/ SCID mice (4 wk old) were purchased from Charles River Japan, Inc. LNCaP, a prostate cancer cell line derived from a lymph node metastasis in a human, was obtained from Riken BRC. LNCaP cells were cultured in RPMI-1640 medium (Wako Pure Chemical Industries) containing 10% fetal bovine serum (Sigma-Aldrich) at 37°C in a humidified incubator containing 5% CO 2 . To create the tumor xenograft models, LNCaP cells were collected in a 1:1 mixture (v/v) of medium and Matrigel (Corning) and subcutaneously implanted into either the left or right shoulder of the mice (;5 • 10 6 cells were inoculated at each site). The tumor xenograft models (n 5 18; body weight, 18.0 6 2.1 g) were evaluated by small-animal PET/CT 40 d after implantation (tumor volume, 82.9 6 64.7 mm 3 ). Male ICR mice (10 wk old; n 5 9; body weight, 39.6 6 2.1 g) were purchased from Japan SLC, Inc., and used as a non-tumor-bearing cohort for biodistribution experiments.