Sk n fi

U-2 OS cells (Sigma-Aldrich) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin. NB cell lines SK-N-FI (Sigma-Aldrich), SK-N-AS (ATCC), SH-SY5Y (CLS Cell Lines Service), CHLA-90 (CCOG), IMR-32 (CLS Cell Lines Service) and SK-N-BE(2) (DSMZ) cells were cultured in specific media. SK-N-FI, SK-N-AS and SH-SY5Y cells were cultured in DMEM supplemented with 10% FBS, 0.1 mM Non-Essential Amino Acids (NEAA), and penicillin/streptomycin. CHLA-90 cells were cultured in DMEM supplemented with 20% FBS, 1× ITS (Gibco) and penicillin/streptomycin. IMR-32 cells were cultured in Minimum Essential Medium (MEM) supplemented with 10% FBS, 1 mM sodium pyruvate, 1× GlutaMAX (Gibco) and penicillin/streptomycin. SK-N-BE(2) cells were cultured in DMEM/F-12 media supplemented with 10% FBS, 1× GlutaMAX and penicillin/streptomycin. All cell lines were routinely tested for mycoplasma contamination and confirmed to be mycoplasma negative.

Culture medium and supplements were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Oleate, palmitate, stearate, linOleate, elaidate, vaccenate, bovine serum albumin, HepG2, HEK293T, and SK-N-FI cells were purchased from Sigma-Aldrich (St. Louis, MO, USA). All of the chemicals that were used in this study were of analytical grade. All of the experiments and measurements were carried out by using Millipore ultrapure water.