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Dual luciferase reporter assay

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The Dual-Luciferase reporter assay is a sensitive and quantitative system used to measure gene expression. It utilizes two different luciferase reporter enzymes, Firefly luciferase and Renilla luciferase, to enable the monitoring of two separate biological processes within the same system.

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Lab products found in correlation

Synergy 2 luminometer, by Agilent Technologies (2 mentions) P50 binding elisa, by Thermo Fisher Scientific (2 mentions) Dual luciferase reporter assay system, by Promega (2 mentions)

2 protocols using dual luciferase reporter assay

1

Quantifying NF-κB Activation Pathways

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NF-κB activity was determined by both p50 binding ELISA (Thermo Scientific) and Dual-Luciferase reporter assay (Qiagen). Binding ELISA was performed according to the manufacturer’s protocol using whole cell lysates and values were normalized to μg protein as determined by protein estimation (Pierce). Dual-Luciferase reporter assay system (Promega) results were determined by Synergy2 luminometer (Biotek).
Nomura A., Gupta V.K., Dauer P., Sharma N.S., Dudeja V., Merchant N., Saluja A.K, & Banerjee S. (2017). NFkB-mediated Invasiveness in CD133+ Pancreatic TICs is Regulated by Autocrine and Paracrine Activation of IL-1 Signaling. Molecular cancer research : MCR, 16(1), 162-172.
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2

NF-κB Activation Quantification Protocols

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NF-κB activity was determined by both p50 binding ELISA (Thermo Scientific) and Dual-Luciferase reporter assay (Qiagen). Binding ELISA was performed according to the manufacturer's protocol using whole cell lysates and values were normalized to μg protein as determined by protein estimation (Pierce). Dual-Luciferase reporter assay system (Promega) results were determined by Synergy2 luminometer (Biotek).
Nomura A., Banerjee S., Chugh R., Dudeja V., Yamamoto M., Vickers S.M, & Saluja A.K. (2015). CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer. Oncotarget, 6(10), 8313-8322.
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