Knockdown of SRSF10 was performed with the siGENOME SMARTpool-Human SRSF10 (2914-02-0005; Dharmacon). The siRNAs against hnRNP F/H (GAACUGAACAAUUUCUUCC) and hnRNP K (UGAUACUCAAUAUGCGCUC) were from previously published work (Garneau et al., 2005 (link); Revil et al., 2009 (link)) and synthesized by IDT. siRNAs were transfected (100 nM) using Lipofectamine 2000 (Invitrogen). Proteins or RNA were extracted 72 hr post-transfection.
Sigenome smartpool human srsf10
Manufactured by Horizon Discovery
SiGENOME SMARTpool-Human SRSF10 is a collection of pre-designed, pooled small interfering RNAs (siRNAs) targeting the SRSF10 gene in humans. The pool is designed to provide effective gene knockdown through RNA interference.
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Lab products found in correlation
3 protocols using sigenome smartpool human srsf10
1
Knockdown of SRSF10 and hnRNP F/H, K
2
Knockdown of SRSF10 and hnRNP F/H, K
3
Plasmid-based Transfection of HeLa and 293 Cells
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Plasmids expressing Bcl-x reporter minigenes (X2.13 and X2), SRSF9 and SRSF1 were described previously (28 (link)–30 (link)). Details of plasmids expressing 3XFlag-SRSF10, Flag-SRSF10 and HA-tagged SRSF10 were also previously described (31 (link)). Plasmid transfections in HeLa-HIV cells (HeLa rtTA-HIV-ΔMls cells as described in references 13 (link),18 (link),19 (link)) or 293 cells (EcR 293 cells from Thermo Fisher Scientific) were carried out with polyethyleneimide (Polysciences Inc.) or Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.
The HIV-YFP plasmid is based on phRL-null (Promega) containing the pNL4.3 HIV promoter and the YFP coding sequence in replacement of the RLuc gene. The CMV-Tat plasmid has been described previously (32 (link)).
The siRNA used to knockdown the expression of SRSF10, siGENOME SMARTpool-Human SRSF10, was purchased from Dharmacon and transfected into cells at a concentration of 100 nM using Lipofectamine 2000 (Invitrogen). Proteins or RNA were extracted from mock-transfected and siRNA-transfected cells at 72 h post-transfection.
The HIV-YFP plasmid is based on phRL-null (Promega) containing the pNL4.3 HIV promoter and the YFP coding sequence in replacement of the RLuc gene. The CMV-Tat plasmid has been described previously (32 (link)).
The siRNA used to knockdown the expression of SRSF10, siGENOME SMARTpool-Human SRSF10, was purchased from Dharmacon and transfected into cells at a concentration of 100 nM using Lipofectamine 2000 (Invitrogen). Proteins or RNA were extracted from mock-transfected and siRNA-transfected cells at 72 h post-transfection.
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