Cobas integra 400 plus analyzer

Manufactured by Roche

Sourced in Switzerland, Germany, United States, Japan, Canada

The Cobas Integra 400 plus analyzer is a clinical chemistry system designed for automated analysis of a wide range of clinical chemistry and immunochemistry tests. It features high-throughput capabilities and advanced technology to ensure accurate and reliable results.

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69 protocols using cobas integra 400 plus analyzer

Comprehensive Blood Analysis Protocol

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Complete blood counts, including leukocyte differentials (CBC/diff), were determined from blood samples collected in EDTA-coated blood tubes and analyzed using a Sysmex XT2000V (Sysmex America, Mundelein, IL). Biochemical analyses of serum samples collected in red-top Vacutainers were performed using the Roche COBAS Integra 400 plus analyzer (Roche Diagnostics, Indianapolis, IN). For coagulation studies, blood was collected in 3.2% sodium citrate-coated vacutainers and processed according to the manufacturer’s recommendations. Plasma from the citrate-coated tubes was analyzed on a STA Compact analyzer (Diagnostica Stago, Parsippany, NJ) for activated partial thromboplastin time (aPTT) and prothrombin time (PT) and for fibrinogen and D-dimer concentrations.

Wahl-Jensen V., Johnson J.C., Lauck M., Weinfurter J.T., Moncla L.H., Weiler A.M., Charlier O., Rojas O., Byrum R., Ragland D.R., Huzella L., Zommer E., Cohen M., Bernbaum J.G., Caì Y., Sanford H.B., Mazur S., Johnson R.F., Qin J., Palacios G.F., Bailey A.L., Jahrling P.B., Goldberg T.L., O’Connor D.H., Friedrich T.C, & Kuhn J.H. (2016). Divergent Simian Arteriviruses Cause Simian Hemorrhagic Fever of Differing Severities in Macaques. mBio, 7(1), e02009-15.

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Fasting Blood Biomarkers and Endothelial Function

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At each visit, a fasting blood sample was drawn in blood tubes without anticoagulant or EDTA-coated to obtain serum and plasma samples, respectively. After separation by centrifugation, the samples were frozen at −80 °C until analysis. T-C, triglycerides (TG) and HDL-C levels, as well as apolipoproteins A1 (Apo A1) and B (Apo B) were determined in serum samples following reference methods or methods recommended by Sociedad Española de Bioquímica Clínica y Patología Molecular (SEQC) using a Roche Cobas Integra 400 plus analyzer (Roche Diagnostics, Mannheim, Germany). LDL and VLDL (very low-density lipoprotein) were calculated according to the Friedewald formula—LDL = T-C − (HDL-C + TG)]; VLDL = TG/5 [27 (link)]—and Apo B/Apo A1, LDL/HDL and T-C/HDL ratios were calculated.
Regarding endothelial function biomarkers, endothelial nitric oxide synthase (eNOS, SEA868Hu), E-selectin (SEA029Hu) and P-selectin (SEA569Hu) plasma concentrations were determined in duplicate by ELISA (Cloud-Clone Kit Corp., Katy, TX, USA) using a Bio-Tek^® Synergy™ HT Multi-Detection Microplate Reader controlled by BioTek^®Gen5 version 2.01.14 software (BioTek Instruments, Winooski, VT, USA).

González-Rámila S., Mateos R., García-Cordero J., Seguido M.A., Bravo-Clemente L, & Sarriá B. (2022). Olive Pomace Oil versus High Oleic Sunflower Oil and Sunflower Oil: A Comparative Study in Healthy and Cardiovascular Risk Humans. Foods, 11(15), 2186.

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Standardized Biomarker Measurement Protocol

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Biological samples were collected, processed, and stored using standardized laboratory protocols [52 (link)]. Venous blood samples (12mL) were collected in 3 vacutainers (i.e., red-top, purple-top dipotassium ethylenediaminetetraacetic acid (K2EDTA), and blue-top metal-free K2EDTA; BD Biosciences) and stored in a portable freezer unit that was set to 4—6 °C (i.e., optimal refrigeration temperature) until processing < 4 h after collection. Complete blood count was analyzed via automated Coulter counter (Coulter HMX). Iron and inflammatory biomarkers were analyzed in batch at St. John's Research Institute in Bangalore, India, after completion of data collection. SF was measured by electrochemiluminescence (E411, Roche Diagnostics Mannheim, USA). sTfR, CRP, and AGP concentrations were analyzed via the Roche COBAS Integra 400 plus analyzer (Roche Diagnostics, Germany).

Finkelstein J.L., Fothergill A., Guetterman H.M., Johnson C.B., Bose B., Qi Y.P., Rose C.E., Williams J.L., Mehta S., Kuriyan R., Bonam W, & Crider K.S. (2022). Iron status and inflammation in women of reproductive age: A population-based biomarker survey and clinical study. Clinical nutrition ESPEN, 49, 483-494.

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Toxicity Assessment of AS-14-GMNPs in Mice

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Healthy 6-week-old 25g ICR mice were used in this study, 10 animals per group. The mice (5 female and 5 male in each group) were administered tail vein injections on days 1, 3 and 5 (3 times total) as follows:
Group 1: Injection of AS-14-GMNPs in 100 μL DPBS (1.6 μg kg^-1);
Group 2: Injection of 100 μL DPBS.
Toxicity was estimated based on changes in blood biochemistry (cholesterol, total protein, alanine amino-transferase, alkaline phosphatase and bilirubin), which were performed using COBAS INTEGRA 400 plus analyzer (Roche Diagnostics, Switzerland). Male and female parameters were analyzed separately. All data were presented as the mean ± standard error of mean.

Belyanina I.V., Zamay T.N., Zamay G.S., Zamay S.S., Kolovskaya O.S., Ivanchenko T.I., Denisenko V.V., Kirichenko A.K., Glazyrin Y.E., Garanzha I.V., Grigorieva V.V., Shabanov A.V., Veprintsev D.V., Sokolov A.E., Sadovskii V.M., Gargaun A., Berezovski M.V, & Kichkailo A.S. (2017). In Vivo Cancer Cells Elimination Guided by Aptamer-Functionalized Gold-Coated Magnetic Nanoparticles and Controlled with Low Frequency Alternating Magnetic Field. Theranostics, 7(13), 3326-3337.

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Evaluating Metabolic and Inflammatory Markers in Mice

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After six weeks of mice interventional feeding, the blood sample was extracted. Preparation included mice fasting the night before blood was drawn with ketamine used as anesthesia. The venous sinus was the chosen location to draw blood, the collected blood was then put in a sterile and dry tube with no anticoagulant and was let to coagulate at room temperature. Then, centrifugation (3,000 rpm, 20 min) was done to obtain the serum. Biomedical analysis (LDL, triglyceride, HDL, total cholesterol, and blood glucose) was performed utilizing the COBAS Integra® 400 plus analyzer (Roche). Blood was also taken from liver tissue through the hepatic portal vein to evaluate superoxide dismutase (SOD) enzyme activities according to the product kit (superoxide dismutase assay kit Sigma-Aldrich). PGC-1α levels were calculated using Sunlong Biotech Co., Ltd.’s PGC-1α Mouse ELISA Kit to quantify PGC-1α concentration from liver tissue. TNF-α levels were calculated using the Mouse Tumor Necrosis Factor α (TNFα) Kit from liver tissue. Interleukin 10 (IL-10) levels were calculated using Abcam's IL-10 ELISA Kit from liver tissue. Serum lipase levels of mice were measured using Mouse Lipase, Pancreatic (PL) ELISA Kit. The body weights of mice were measured using digital scales.

Permatasari H.K., Nurkolis F., Gunawan W.B., Yusuf V.M., Yusuf M., Kusuma R.J., Sabrina N., Muharram F.R., Taslim N.A., Mayulu N., Batubara S.C., Samtiya M., Hardinsyah H, & Tsopmo A. (2022). Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha. Current Research in Food Science, 5, 1251-1265.

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Comprehensive Metabolic Profile in Rats

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After the experimental program, the rats were fasted overnight and euthanized under anesthesia using 100 mg/kg body weight (bw) ketamine administered intraperitoneally. Heart blood was collected in two distinctive tubes: one with EDTA (ethylenediamine tetraacetic acid) anticoagulant for glycosylated hemoglobin determination and another deprived of anticoagulant for serum separation.
The biochemical parameters were carried out in an authorized sanitary veterinary laboratory, Synevovet, Chiajna, Romania. ALT, AST, total cholesterol, LDL cholesterol, triglycerides, and creatinine levels were spectrophotometrically assayed using a Cobas Integra 400 plus analyzer, Roche Diagnostics, Indianapolis-Marion County, Indiana, USA. HbA1c measurements were performed using a standardized immunoturbidimetry method (GSP/DCCT; National Glycohemoglobin Standardization Program/Diabetes Control and Complications Trial reference method) with Cobas Integra 6000 analyzer, Roche Diagnostics, Indianapolis-Marion County, Indiana, USA. HDL cholesterol and serum urea activities were determined by a spectrophotometric assay using the same chemistry analyzer. The uric acid and blood urea nitrogen concentrations were measured using VetTest 8008 serum chemistry analyzer (Idexx Laboratories, Westbrook, Maine, USA).

Draganescu D., Andritoiu C., Hritcu D., Dodi G, & Popa M.I. (2021). Flaxseed Lignans and Polyphenols Enhanced Activity in Streptozotocin-Induced Diabetic Rats. Biology, 10(1), 43.

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HbA1c Measurement via Cobas Integra 400

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Blood samples were collected and sent to the nationally certified pathology laboratory on the day of collection for HbA1c measurement using Cobas Integra 400 plus analyzer (Roche Diagnostics, Laval, QC, Canada), with a measuring range of 4.3–18.8% and a coefficient variance of 5%.

Ng Y.T., Phang S.C., Tan G.C., Ng E.Y., Botross Henien N.P., M. Palanisamy U.D., Ahmad B, & Abdul Kadir K. (2020). The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial. Nutrients, 12(5), 1522.

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Lipoprotein Analysis and Sterol Profiling

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Blood was collected at the end of each treatment period following a 12-hour fast. Plasma total cholesterol, TG, high density lipoprotein cholesterol (HDL-C), apoC-III, and apoE were measured enzymatically on a Cobas Integra 400 plus analyzer using Roche Diagnostics Systems reagents. Plasma LDL-C levels were estimated using the Friedewald formula. Cholesterol and TG were also measured enzymatically in VLDL, IDL, LDL, and HDL isolated by ultracentrifugation.¹² ApoB in plasma and in VLDL, IDL, and LDL was measured using an apoB enzyme-linked immunosorbent assay (ELISA) kit (AlerCheck, Inc.). Plasma Lp(a) levels were measured using a monoclonal antibody-based ELISA method validated to accurately measure Lp(a) independently of the number of repeats in Kringle IV type 2.^{13 (link)} Apo(a) isoforms were assayed with methodology previously described,^{14 (link)} in which the isoform size visualized on agarose gel electrophoresis is directly proportional to the number of Kringle IV type 2 repeats. In some subjects, only one apo(a) isoform is expressed; in most individuals, however, apo(a) is expressed by both alleles, with one isoform more prevalent than the other. Particle sizes and particle number were determined by ion mobility analysis.^{15 (link)} Lathosterol, campesterol, and β-sitosterol were measured by gas chromatography as previously described.^{16 (link)}

Reyes-Soffer G., Pavlyha M., Ngai C., Thomas T., Holleran S., Ramakrishnan R., Karmally W., Nandakumar R., Fontanez N., Obunike J., Marcovina S.M., Lichtenstein A.H., Matthan N.R., Matta J., Maroccia M., Becue F., Poitiers F., Swanson B., Cowan L., Sasiela W.J., Surks H.K, & Ginsberg H.N. (2017). Effects of PCSK9 Inhibition With Alirocumab on Lipoprotein Metabolism in Healthy Humans. Circulation, 135(4), 352-362.

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Lipoprotein Profile Measurement Protocol

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Lipoprotein profile, including triglycerides (TG), low-density lipoproteins (LDL), high-density lipoproteins (HDL), and cholesterol, was measured using diagnostic Cobas c pack reagents kits (Roche Diagnostics, Indianapolis, IN, USA) according to the manufacturer’s instructions applied on COBAS INTEGRA 400 plus analyzer (Roche Diagnostics GmbH, USA).

Zahran E., Elbahnaswy S., Ahmed F., Ibrahim I., Khaled A.A, & Eldessouki E.A. (2023). Nutritional and immunological evaluation of Nannochloropsis oculata as a potential Nile tilapia-aquafeed supplement. BMC Veterinary Research, 19, 65.

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Toxicity Assessment of As42-GMNPs in Mice

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Six-week-old, 25-g ICR male mice were used in this study; 10 animals per group. The tail-vein injections on the mice (5 female and 5 male in each group) were performed on days 1, 3, and 5 (3 times total) were as follows:

Group 1: injections of As42-GMNPs in 100 μL DPBS (1.6 μg/kg); and

Group 2: injections of 100 μL DPBS.

Toxicity was estimated by the changes in blood biochemistry (cholesterol, total protein, ALT, AST, and bilirubin); this was performed using the COBAS INTEGRA 400 plus analyzer (Roche Diagnostics, Switzerland). Parameters for male and female mice were analyzed separately. All data were presented as the mean ± SEM.

Kolovskaya O.S., Zamay T.N., Belyanina I.V., Karlova E., Garanzha I., Aleksandrovsky A.S., Kirichenko A., Dubynina A.V., Sokolov A.E., Zamay G.S., Glazyrin Y.E., Zamay S., Ivanchenko T., Chanchikova N., Tokarev N., Shepelevich N., Ozerskaya A., Badrin E., Belugin K., Belkin S., Zabluda V., Gargaun A., Berezovski M.V, & Kichkailo A.S. (2017). Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer. Molecular Therapy. Nucleic Acids, 9, 12-21.

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