Apoptosis was assessed by Flow Cytometry Apoptosis Detection Kit (BD Biosciences, Franklin Lakes, NJ, USA). Briefly, harvested cells were washed with cold PBS and resuspended in the provided binding buffer. Following this, cells were subjected to a 15-min staining process with Annexin V-FITC and propidium iodide (PI) at room temperature, ensuring the process was conducted in darkness. Following the staining procedure, flow cytometry was employed to analyze the apoptotic rates in the cells.
Flow cytometry apoptosis detection kit
Manufactured by BD
Sourced in United States
The Flow Cytometry Apoptosis Detection Kit is a laboratory instrument designed to analyze and quantify the process of apoptosis, or programmed cell death, in cell populations. The kit utilizes flow cytometry technology to detect and measure various markers associated with the apoptotic process.
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2 protocols using flow cytometry apoptosis detection kit
1
Apoptosis Detection by Flow Cytometry
2
Chondrocyte Apoptosis Detection by Flow Cytometry
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A flow cytometry apoptosis detection kit (BD, USA) was used to verify and detect chondrocyte apoptosis resulting from different experimental treatments. Briefly, chondrocytes were initially digested and collected using pancreatic enzymes. Subsequently, the binding buffer (300 μl) and annexin V–fluorescein isothiocyanate (15 μl) were added following the kit instructions, and the cells were incubated at 25°C in the dark for 15 min. Last, propidium iodide (5 μl) was added, and chondrocyte apoptosis was detected using flow cytometry (FACSCalibur, USA).
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