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Elisa reader

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The ELISA reader is a laboratory instrument used to measure the optical density of solutions in enzyme-linked immunosorbent assay (ELISA) microplates. It is designed to detect and quantify the presence of specific proteins, antibodies, or other biomolecules in samples. The device reads the absorbance of the colored product produced in the ELISA reaction, providing a quantitative measurement of the target analyte.

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Lab products found in correlation

Elisa kit for leptin, by Cloud-Clone (1 mentions) Elisa kit, by Cloud-Clone (1 mentions) Sea133ra, by Cloud-Clone (1 mentions)

10 protocols using elisa reader

1

Quantification of Thyroid Hormones and Appetite Regulators

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The 125I radioimmunoassay (RIA) kits of T3 and T4 (Institute of Atomic Energy, Beijing, China) that were previously validated for Mongolian gerbil were used to quantify serum T3 and T4 concentrations. The intra-assay coefficients of variation (CV) were 5.1% for T3 and 4.4% for T4 (44 (link)).
Serum ghrelin levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (catalog no. CEA991Ra; Cloud-Clone Corp.), and serum leptin levels were measured using an ELISA kit for leptin (catalog no. SEA084Ra; Cloud-Clone Corp.) according to the manufacturer’s instructions. Absorbance was measured at 450 nm against a blank using an ELISA reader (RayBiotech, Canada). The intra- and interassay CVs were <10% and <15% for both kits. The minimum detectable dose for ghrelin was 52.3 pg/ml, and that for leptin was 0.129 ng/ml.
Khakisahneh S., Zhang X.Y., Nouri Z, & Wang D.H. (2020). Gut Microbiota and Host Thermoregulation in Response to Ambient Temperature Fluctuations. mSystems, 5(5), e00514-20.
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2

ELISA-Based Serum TNF-α Quantification

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Serum TNF‐α levels were detected using an ELISA kit (SEA133Ra, Cloud‐Clone Corp., Wuhan, China) according to the manufacturer’s instructions. Absorbance was measured at 450 nm against a blank using an ELISA reader (RayBiotech, Canada). Assay results were expressed as pg ml‐1.
Khakisahneh S., Zhang X., Nouri Z, & Wang D. (2021). Cecal microbial transplantation attenuates hyperthyroid‐induced thermogenesis in Mongolian gerbils. Microbial Biotechnology, 15(3), 817-831.
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3

Nitric Oxide Quantification via Griess Assay

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Griess assay [44 (link), 45 (link)] was used to identify NO level, so that 100 µl of the sample was mixed with 100 µl acidic Griess reagent and the absorbance was determined by an ELISA reader (RayBiotech, Canada) at 540 nm.
Shabani E., Kalantari H., Kalantar M., Goudarzi M., Mansouri E, & Kalantar H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BMC Complementary Medicine and Therapies, 21, 301.
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4

Glutathione Quantification by Ellman's Method

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We computed GSH level by Ellman,s technique [46 (link)–48 ]. In summary, supernatant 1 mL was blended with 1 mL of 4% sulfosalicylic acid, then it was centrifuged at (1200 g) for a quarter-hour at 4 °C. Then, 2.7 mL of 0.1 M phosphate buffer (pH 7.4) and 0.2 mL of 5,5-dithiobis 2-nitrobenzoic acid (DTNB) (40 mg/10 mL of 0.1 M phosphate buffer, pH 7.4) were mixed. Afterward, the yellow color was investigated instantly at 412 nm by an ELISA reader (RayBiotech, Canada).
Shabani E., Kalantari H., Kalantar M., Goudarzi M., Mansouri E, & Kalantar H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BMC Complementary Medicine and Therapies, 21, 301.
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5

Quantification of VEGF, TGF-β1, and sFas

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Vascular endothelial growth factor (VEGF) was assayed by the method described in the commercial VEGF ELISA kit instructions (Peninsula Inc., USA), while transforming growth factor beta 1 (TGF-β1) was assayed according to the method described in commercial kit from Abcam Company (USA). The level of soluble Fas (sFas) was quantitated by the sandwich ELISA kit according to manufacture instructions (Biosource International, Camarillo, California, USA). Absorbance was measured at 450 nm against blank using an ELISA reader (RayBiotech, Canada).
Demerdash H.M., Hussien H.M., Hassouna E, & Arida E.A. (2017). Detection of MicroRNA in Hepatic Cirrhosis and Hepatocellular Carcinoma in Hepatitis C Genotype-4 in Egyptian Patients. BioMed Research International, 2017, 1806069.
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6

Quantifying Lipid Peroxidation via TBARS

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Lipid peroxidation was computed using estimating colorimetric of thiobarbituric acid-reactive substance (TBARS) following the reaction of malondialdehyde with thiobarbituric acid. In summary, 500 µl of tissue supernatant was mixed with one ml trichloroacetic acid (TCA) (20%). Then, for 10 min, it was centrifuged (2000 g). One ml of the supernatant was mixed with 0.5 ml of 0.67% thiobarbituric acid (TBA) and heated for 30 min in a boiling water bath, then cooled and the absorbance was determined by an ELISA reader (RayBiotech, Canada) at 532 nm [42 (link), 43 ].
Shabani E., Kalantari H., Kalantar M., Goudarzi M., Mansouri E, & Kalantar H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BMC Complementary Medicine and Therapies, 21, 301.
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7

Protein Quantification via Biuret Method

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By applying the Biuret method (Ernest, 1996), we measured the protein content. It is worth noting that bovine serum albumin (BSA) was administered as the main criterion. So that 2.9 mL of normal saline and 3 mL of Biuret reagent were mixed in 0.1 ml of the sample. Afterward, the content was put at the temperature room for 10 min in order to measure the absorbance rate at 540 nm by an ELISA reader (RayBiotech, Canada). A BSA standard curve was used to measure the protein concentration [41 ]
Shabani E., Kalantari H., Kalantar M., Goudarzi M., Mansouri E, & Kalantar H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BMC Complementary Medicine and Therapies, 21, 301.
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8

Catalase Activity Assay Using H2O2 Decomposition

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By a technique that measures the decomposition of hydrogen peroxide (H2O2), the function of CAT was evaluated [49 (link), 50 ]. In summary, the sample contained 1.95 mL of phosphate buffer (0.05 M, pH 7), 1 mL of hydrogen peroxide (0.019 M), and 0.05 mL supernatant. Afterward, the absorbance was determined immediately at 240 nm by an ELISA reader (RayBiotech, Canada).
Shabani E., Kalantari H., Kalantar M., Goudarzi M., Mansouri E, & Kalantar H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BMC Complementary Medicine and Therapies, 21, 301.
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9

Measuring Hepatic DIO2 Levels

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The DIO2 level in the liver was also measured using an ELISA kit (SEC903Ra, Cloud‐Clone Corp., Wuhan, China) according to the manufacturer’s instructions. Absorbance was measured at 450 nm against a blank using an ELISA reader (RayBiotech, Canada). The minimum detectable value of the kit was 0.059 ng ml‐1. The intra‐ and inter‐assay coefficients of variation (CV) for DIO2 were 10% and 12%, respectively. Assay results were expressed as ng ml‐1.
Khakisahneh S., Zhang X., Nouri Z, & Wang D. (2021). Cecal microbial transplantation attenuates hyperthyroid‐induced thermogenesis in Mongolian gerbils. Microbial Biotechnology, 15(3), 817-831.
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10

Cytokine Quantification by ELISA

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The concentrations of interleukin 17 (IL-17) and transforming growth factor beta 1 (TGF-β1) were determined by enzyme-linked immunosorbent assay (ELISA) according to manufacturer's instructions (SunRed; hepatology company; Shangahai, China).
Absorbance was measured at 450nm against blank using an ELISA reader (Ray Biotech, Canada).
Elbanan W.K., Fathy S.A., Ibrahim R.A, & Hegazy M.G.A. (2020). Assessment of interleukin 17 and transforming growth factor-beta 1 in hepatitis C patients with disease progression. Tropical biomedicine, 37(4).
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