Tensor 27 ft ir spectrophotometer

The tensor 27 FT-IR spectrophotometer (Bruker, Munich, Germany) was used for recording the infrared spectrum of the specimens, at wave numbers in the range of 4000–400 cm⁻¹ with the resolution of 4 cm⁻¹ for 100 scans, according to the method described by Perez-Mateos et al. [26 (link)].

All manipulations were carried out using standard Schlenk techniques and vacuum-line manipulations under a protective atmosphere of nitrogen unless otherwise stated. Compound 1 was prepared according to the published procedure [43 (link)]. All other compounds were purchased from commercial sources unless otherwise specified. The solvents were distilled prior to use and were deaerated according to standard procedures [66 ]. The IR spectra were recorded as KBr disks using a Bruker Tensor 27 FTIR spectrophotometer. UV–vis spectra were recorded on a Jasco V-670 UV/vis/near-IR spectrophotometer. Elemental analyses were carried out on a Vario EL elemental analyzer. NMR spectra were recorded on a Bruker AVANCE DPX-200 spectrometer at 300 K. ¹H and ¹³C chemical shifts refer to solvent signals. ESIMS spectra were recorded on a Bruker Daltronics ESQUIRE3000 PLUS spectrometer. Temperature-dependent magnetic susceptibility measurements on powdered solid samples were carried out using a MPMS 7XL SQUID magnetometer (Quantum Design) over a temperature range 2–330 K at an applied magnetic field of 0.1, 0.5, and 1.0 Tesla. The observed susceptibility data were corrected for underlying diamagnetism.
Safety note! Perchlorate salts of transition metal complexes are hazardous and may explode. Only small quantities should be prepared and great care should be taken.

A Bruker Tensor 27 FT-IR spectrophotometer with an ATR attachment was employed. The measurements were performed in the range of 4000–650 cm⁻¹ and spectra were analysed using OPUS software (Bruker Corporation, Germany).

Cd(CH₃COO)₂.2H₂O and 5-aminotetrazole, cisplatin (cis-diamminedichloroplatinum(II)) were obtained from Sigma Aldrich, CA, USA. Culturing medium DMEM^TM and phenol red free medium DMEM/F12, PBS, FBS, FCS, Trypsin, Accutase enzyme, Penicillin, Presto- Blue²⁷ , Annexin V, propidium iodide (PI) solutions, RNA isolation and cDNA synthesis kits, primers, antibodies and Western blot reagents were obtained from Thermo Fisher Scientific and Gibco/Invitrogen, Breda, the Netherlands.
FT-IR spectra were determined on a Bruker Tensor 27 FT-IR spectrophotometer with KBr disks in the range of 4000–400 cm⁻¹ (Columbia, MD, USA). QTOF LC/MS spectrometer was used for mass spectrometry (Billerica, MA, USA). ¹HNMR spectroscopy were performed at room temperature on the NMR Bruker Advance 400 MHz magnet (Agilent, USA) using DMSO-d₆. Elemental analysis, C H N, was performed by Elemental analyzer Vario EL III (Sydney, Australia). X- ray crystallography was performed on an SuperNova, Dual, Cu at zero, Atlas diffractometer, using Cu Kα radiation λ = 1.54184 Å at 130 K. Facs Canto II (Bio Rad) flow cytometry system (Mexico, USA) with Flow Logic TM program, was used for cell cycle AnnexinV and PI evaluation. Quantitative PCR (qPCR) was performed by Mic qPCR, Bio Molecular system (Santa Clara, USA).