Hp 5 column

Manufactured by Agilent Technologies

Sourced in United States, Japan, United Kingdom

The HP-5 column is a capillary column used in gas chromatography. It is designed to separate a wide range of organic compounds, including hydrocarbons, alcohols, and esters. The column has a 5% phenyl-methylpolysiloxane stationary phase, which provides good separation and peak shape for a variety of analytes.

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Lab products found in correlation

132 protocols using hp 5 column

Sorption Kinetics of Ethyl Formate in Grape Processing

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The concentration and time (CT) values were calculated by collecting gases at 0.5 h, 1 h, 2 h, and 4 h after EF treatment [35 ]. A total of 50 mL of EF gas in each 12 L desiccator was collected in a Tedlar gas sampling bag (1 L, SKC, Dorset, UK) using a syringe (100 mL, Hamilton, NV, USA). The EF concentrations were analyzed using gas chromatography (GC; Agilent Technology 6890N, Agilent Technology, Santa Clara, CA, USA) with the following conditions: flame ionization detector (FID) injector temperature of 200 °C, oven temperature of 100 °C, and detector temperature of 240 °C while utilizing an HP-5 column (0.32 mm × 30 m, Agilent Technology, Santa Clara, CA, USA).
The sorption ratio of EF was determined at grape loading ratios of 0%, 5%, 10%, 15%, and 20% (w/v) using a 12 L desiccator. All sorption experiments included treatment with 20 mg/L EF at 20 °C for 4 h. The gas concentrations for sorption were determined at 10 min, 30 min, 1 h, 1.5 h, 2 h, 3 h, and 4 h after treatment. C/C₀ values were calculated as the concentration at each time point after treatment (C) divided by the concentration 10 min after treatment (C₀). A 12 L desiccator without grapes was used as the control (0%).

Jeon J.C., Kim H.K., Koo H.N., Kim B.S., Yang J.O, & Kim G.H. (2022). Synergistic Effect of Cold Treatment Combined with Ethyl Formate Fumigation against Drosophila suzukii (Diptera: Drosophilidae). Insects, 13(8), 664.

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Ethylene and ABA Production in Seedlings

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Seedlings (using about 20 individual seedlings) were grown in MS with or without 150 ml distilled water. Using 3 ml disposable syringe, we harvested capped air in headspace containing ethylene, then sealed by parafilm. Using Hamilton syringe, 100 μl gas was extracted from sealed air, then feeding to gas chromatography with flame ionization detector (Agilent 7890B GC). We used HP-5 column (#19091J-413, Agilent). Ethylene production was normalized by seedling weight. For measuring ABA contents, we used ABA ELISA kit (CSB-E09159Pl). Intensity of 450 nm fluorescence was determined by using Plate reader-Powerwave X (Bio-Tek). ABA production was normalized by sample weight. The measurements were performed from three biological replicates and two technical replicates each.

Kim J., Joo Y., Kyung J., Jeon M., Park J.Y., Lee H.G., Chung D.S., Lee E, & Lee I. (2018). A molecular basis behind heterophylly in an amphibious plant, Ranunculus trichophyllus. PLoS Genetics, 14(2), e1007208.

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Quantifying Chlorinated Solvents and Gases

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1,1,2,2-tetrachloroethene (≥99.5%), 1,1,2-trichloroethene (99.5%), trans-1,2-dichloroethene (trans-1,2-DCE; ≥98%), 1,1-dichloroethene (1,1-DCE; ≥99%), cis-1,2-dichloroethene (cis-1,2-DCE; ≥97%), 1,2-dichloroethane (1,2-DCA; ≥99%) and vinyl chloride (VC; 100 μg/mL in methanol) were purchased from Sigma-Aldrich (St. Louis, MO, United States). All other chemicals used to prepare analytical standards or feed solutions were analytical reagent grade.
Two milliliters of liquid samples obtained from the cathode chambers were immediately transferred to sealed 10 mL bottles filled with the high purity N₂ gas (≥99.99%). The bottles were placed in a 25°C shaker for 30 min to reach the equilibrium. Concentrations of volatile organic compounds including PCE, TCE, trans-1,2-DCE, 1,1-DCE, cis-1,2-DCE, 1,2-DCA, and VC in the headspace (8 mL) were determined using a gas chromatograph (Agilent 7890A, Palo Alto, CA, United States) equipped with a 63Ni electron capture detector and DB-1301 column (30 m × 250 μm × 0.25 μm, Agilent). Ethene and methane were determined using a gas chromatograph (Agilent 7890A, Palo Alto, CA, United States) equipped with flame ionization detector (FID) and HP-5 column (30 m × 250 μm × 0.25 μm, Agilent). Headspace concentrations were converted to aqueous-phase using tabulated Henry’s law constants (Chen et al., 2018 (link)).

Chen F., Li Z., Yang J., Liang B., Huang C., Cai W., Nan J, & Wang A. (2018). Electron Fluxes in Biocathode Bioelectrochemical Systems Performing Dechlorination of Chlorinated Aliphatic Hydrocarbons. Frontiers in Microbiology, 9, 2306.

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Hydrogenation of 5-Hydroxymethylfurfural

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The HMF hydrogenation reactions were carried out in a 100 mL stainless steel autoclave equipped with a mechanical stirrer and a thermocouple. In a typical experiment, 0.1550 g of HMF (Sigma-Aldrich, 99%) were dissolved in 15 mL of 2-butanol (Sigma-Aldrich, 99%). The solution was placed into a glass inlet with the appropriate amount of activated catalyst (Ru:HMF molar ratio of 1:100). The system was then sealed and purged with N₂ first and then H₂, which pressurized the reactor to the desired pressure (20 bar). The autoclave was heated up to 150 °C and the solution was stirred at 1000 rpm. Samplings were carried out by stopping the stirring and quenching of the reaction of the samples in an ice bath. A sample of the reaction solution (ca. 500 µL) was withdrawn and centrifuged in order to separate the catalyst from the solution. The filtrate was diluted with a solution of an external standard (dodecanol, Sigma-Aldrich, >98%) for GC measurement. Product analysis was carried out with a GC-MS (Thermo Scientific, Waltham, MA, USA, ISQ QD equipped with an Agilent VF-5ms column) and the resulting fragmentation peaks were compared with standards present in the software database. Product quantification was carried out through a GC-FID equipped with a non-polar column (Thermo Scientific, TRACE 1300 equipped with an Agilent HP-5 column).

Jouve A., Cattaneo S., Capelli S., Stucchi M., Evangelisti C., Villa A, & Prati L. (2019). CNF-Functionalization as Versatile Tool for Tuning Activity in Cellulose-Derived Product Hydrogenation. Molecules, 24(2), 316.

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Gas Chromatography-Mass Spectrometry Analysis

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Chromatographic gas model Agilent Technologies-7890A was equipped with HP-5 column with (30 m × 0.32 mm (ID) × 0.25 μm (FT, was used. The temperature of the oven was planned as follows: from 60 (held isothermally for 2 min) to 210 °C with ramp: 3 °C/min, then increased to 240 °C with ramp 20 °C/min and the final temperature kept for 8.5 min. Run time: 60 min. Electron ionization energy was 70 eV in the electronic ionization (EI) mode, ion-source: 230 °C, Detector: 290 °C MS, Interface line temperature: 280 °C, Injector: 280 °C, Split ratio: 1:50, EO was diluted with hexane (1:100 ratio) and helium was used as carrier gas (flow rate, 1 mL/min), mass range: 50–480 m/z, and the injection volume of samples were 1.0 μl. The compounds were detected and identified by retention indices (RI), Wiley MS data system library (Wiley, Chichester, UK) and previous literature. Finally, the percentage of constituents was measured based on the peak areas obtained by the response factor of the detector (Adams 2007 ).

Hazrati S., Khurizadeh S, & Sadeghi A.R. (2021). Application of zeolite improves water and nitrogen use efficiency while increasing essential oil yield and quality of Salvia officinalis under water-deficit stress. Saudi Journal of Biological Sciences, 29(3), 1707-1716.

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Analytical Methods for Chiral Compounds

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GC analyses were performed on a 7890A GC system (Agilent), equipped with an FID detector. HPLC analyses were carried on a 1100A HPLC system (Agilent), equipped with an VWD detector. The product yield and ee value of (R)-levodione were determined by GC using a CP-ChiraSil-DEX CB column (25 m × 0.25 mm × 0.25 μm, Agilent) as described previously.^{3,11 (link)} The product yield and de value of dihydrocarvone were determined by GC using a DB-5 column (30 m × 0.32 mm × 0.25 μm, Agilent) as described previously.^{3 (link)} The yield and ee value of 2-methyl-hydrocinnamaldehyde (12b) was determined by HPLC using a Chiralcel OJ-H column (250 mm × 4.6 mm, Daicel, Shanghai, China) as described previously.^{11 (link)} The conversion of citral (12a) was performed on GC using a HP-5 column (30 m × 0.32 mm × 0.25 μm, Agilent) as described previously.^{3 (link)} The ee value of citronellal was determined by GC using a Beta DEX 225 column (30 m × 0.25 mm × 0.25 μm, Supelco, Bellafonte, PA, USA) as described previously.^{3 (link)}

Zhang B., Sun J., Zheng Y., Mao X., Lin J, & Wei D. (2022). Identification of a novel ene reductase from Pichia angusta with potential application in (R)-levodione production. RSC Advances, 12(22), 13924-13931.

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Fecal Shield Chemical Analysis by GC-MS

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We used gas chromatography–mass spectrometry (GC–MS) (Agilent Technologies 6890 N with 5896 MS) to analyze the chemicals extracted from the fecal shields. The GC instrument was equipped with an HP5 column (30 m, 0.25-mm internal diameter, 0.25-μm film thickness, Agilent Technologies, Inc., Palo Alto, CA, USA). An aliquot of 2 μL per sample was injected in splitless mode at 220°C, and the temperature was programmed at 50°C for 1 min, then increased to 90°C at 10°C per min, increased to 250°C at 5°C per min, and held at 250°C for 5 min. The temperature of the detector was 250°C. Windows NT/MASS Spectral Search Program (version 1.7) software was used to collect data. The n-alkane (C6-C40) standard was also injected to calculate retention indices (RIs). Individual compounds were identified by integrative analysis of their MS profiles (Doolittle et al. 1995 (link)) and RIs (Carlson et al. 1998 ). Five replicates were conducted for both hexane extracts and DCM extracts. The peaks with a mean relative proportion of > 0.1% in at least one treatment were used for further analysis.

Huang Z.Z., Dong Z.Q., Liang Z.L., Zhang B., Xue H.J, & Ge S.Q. (2022). The fecal shield is a double-edged sword for larvae of a leaf beetle. Current Zoology, 69(2), 173-180.

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Quantification of Amorphadiene in Plants

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Leaves from the greenhouse-grown wild-type and DT plants (μ1 g) were crushed in liquid nitrogen and extracted with ethyl acetate (2 ml) for 30 min in a water bath sonicator (Branson Ultrasonic, Danbury, USA). The extracts were concentrated by evaporation under nitrogen. A 1-μl aliquot from the extract was analyzed by GC–MS, using an Agilent 7890A with an HP-5 column (30 m × 0.25 mm internal diameter, 0.25 μm film thickness) and a 7000C triple quadrupole GC–MS/MS spectrometer. The MS was operated in electron ionization mode and scanning mode (50–500 m/z). Amorphadiene was detected by comparing the retention time and mass spectra with an authentic standard (kindly provided by Amyris Biotechnology, Emeryville, CA, USA).

Malhotra K., Subramaniyan M., Rawat K., Kalamuddin M., Qureshi M.I., Malhotra P., Mohmmed A., Cornish K., Daniell H, & Kumar S. (2016). Compartmentalized Metabolic Engineering for Artemisinin Biosynthesis and Effective Malaria Treatment by Oral Delivery of Plant Cells. Molecular plant, 9(11), 1464-1477.

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GC Analysis of Monomer Conversion

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Samples were measured with GC to determine the monomer conversion from the ratio of the integrals from the monomer and the internal standard. GC was performed on an Agilent 7890A system (Santa Clara, CA, USA), which was equipped with a VWR Carrier-160 hydrogen generator (Radnor, PA, USA), an FID detector and an Agilent HP-5 column of 30 m length and 0.320 mm diameter. The inlet was set to 250 °C with a split injection ratio of 25:1. The carrier gas was hydrogen at a flow rate of 2 mL/min. The temperature program used was injection at 50 °C, after which the temperature was increased by 20 °C/min from 50 to 120 °C, followed by a ramp of 50 °C/min to 300 °C.

Voorhaar L, & Hoogenboom R. (2017). One-Pot Synthesis of Charged Amphiphilic Diblock and Triblock Copolymers Via High-Throughput Cu(0)-Mediated Polymerization. Polymers, 9(8), 320.

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GC-MS Analysis of Essential Oil Volatiles

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Identification of the essential oil volatile components was carried out with a gas chromatography-mass spectrometry (GC-MS) system (7890B GC/5977AMSD; Agilent Technologies Inc., Santa Clara, CA, USA), fitted with a 30 m × 0.25 mm × 0.25 µm HP-5 column (Agilent). Samples of 0.3 µL were injected in the split mode at a ratio of 1:150. The oven temperature was programmed as follows: 65 °C hold for seven min, then 5 °C/min to 200 °C, hold for 10 min, and finally, 30 °C/min to 250 °C and hold for 10 min. Helium was used as carrier gas at a flow rate of 1.17 mL/min. The injector and transfer line temperatures were 250 °C and 280 °C, respectively. Mass spectra matches were made by comparing experimental mass spectra with those of the NIST14 computer library.
Semi-quantification of the volatile substances was performed using gas chromatography coupled to a flame ionization detector (6890 N, Agilent) fitted with the same column and operated under the same conditions as the GC-MS. Quantification was computed as the percentage contribution of each compound to the total amount present. The analysis was repeated twice for each sample.

Somrani M., Inglés M.C., Debbabi H., Abidi F, & Palop A. (2020). Garlic, Onion, and Cinnamon Essential Oil Anti-Biofilms’ Effect against Listeria monocytogenes. Foods, 9(5), 567.

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