Trpv1 cre mice

Manufactured by Jackson ImmunoResearch

Trpv1-Cre mice are a transgenic mouse line that expresses Cre recombinase under the control of the Trpv1 gene promoter. Trpv1 encodes the transient receptor potential cation channel subfamily V member 1, which is involved in the detection of noxious heat and certain chemical stimuli. The Trpv1-Cre mice allow for the selective genetic manipulation of cells and tissues that express Trpv1.

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4 protocols using trpv1 cre mice

Lineage Tracing of Lgr5+ and Lgr6+ Cells

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All procedures involving animal subjects were performed with the
approval of the Institutional Animal Care and Use Committee (IACUC) of the
University of Pennsylvania. The following strains were obtained from Jackson
laboratories: Lgr6-EGFP-IRES-CreERT2 (Lgr6^GFP in
text, Stock No. #016934), Lgr5-EGFP-IRES-CreERT2 (or
Lgr5^GFP in text, Stock No. #008875),
TrpV1^Cre mice (Stock No. #017769),
R26^{loxP-stop-loxP-tTA}(R26^−tTA in text, Stock No.
#008600), TetO-H2BGFP (Stock No. #005104),
R26^{loxP-stop-loxP-tdTom} (Stock No
#007908), R26^{loxP-nTomato-stop-loxP-nGFP} (Stock
No #023035) and R26^{loxP-stop-loxP-DTA} (Stock No
#009669). K14-H2B-PAGFP (K14^H2BPAGFP in text)
mice were generated by the Center for Animal Transgenesis and Germ Cell
Research, at the School of Veterinary Medicine of the University of
Pennsylvania. All mice that were used in this study were bred for multiple
generations into a Crl:CD1(ICR) mixed background. Mice between 2-6 months of
age were used for experiments, with equal male/female representation. There
was no apparent difference in phenotype between genders. Mice were housed
under standard laboratory conditions and received food and water ad
libitum.

Huang S., Kuri P., Aubert Y., Brewster M., Li N., Farrelly O., Rice G., Bae H., Prouty S., Dentchev T., Luo W., Capell B.C, & Rompolas P. (2021). Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization. Cell stem cell, 28(9), 1582-1596.e6.

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Mouse Models for Immunology and Disease Research

Cited 3 times

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C57BL/6, Rag1^−/− mice, Trpv1-Cre mice, Rosa26^tdTomato mice, mut-Stat3 mice (Steward-Tharp et al., 2014 (link)), zDC^DTR mice (Meredith et al., 2012 (link)), Csf1r^DTR mice, Lang^DTR mice (Kissenpfennig et al., 2005 (link)) were obtained from The Jackson Laboratory. Il31^−/− mice (Takamori et al., 2018 (link)) were obtained from Dr. Nakae’s Lab. Tgfbr1^f/fErt2-Cre, Smad3^−/− (on a C57BL/6 background) were previously described and bred in our facility under specific pathogen-free conditions. Trpv1^tdTomato mice were generated in-house by crossing Trpv1-Cre mice with Rosa26^tdTomato mice. Csf1r^DTRLys2-Cre mice (MM^DTR mice) (Schreiber et al., 2013 (link)) were generated in-house by crossing Lys2-Cre mice with Csf1r^DTR mice. Tgfbr1^f/fCd11c-Cre⁺ mice were generated in-house by crossing Cd11c-Cre mice with Tgfbr1^f/f mice. Tgfbr1^f/fLyz2-Cre⁺ were generated in-house by crossing Lyz2-Cre mice with Tgfbr1^f/f mice. Tgfbr1^f/fErt2-Cre mice were treated with tamoxifen (1 μg/mouse) per day for 5 days to delete TβRI. All mice used for experiments were aged 6-12 weeks, both male and female. All animal studies were performed according to National Institutes of Health (NIH) guidelines for use and care of live animals and approved by the Animal Care and Use Committees of National Institute of Dental and Craniofacial Research (NIDCR).

Xu J., Zanvit P., Hu L., Tseng P.Y., Liu N., Wang F., Liu O., Zhang D., Jin W., Guo N., Han Y., Yin J., Cain A., Hoon M.A., Wang S, & Chen W. (2020). The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching. Immunity, 53(2), 371-383.e5.

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Genetic Mouse Models for Pain Research

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Adult mice (8–16 weeks) of both sexes were used in this study, unless specifically described. Il23^−/− knockout (KO) mice and Il23r^−/− (KO) mice were provided by Genentech under the Material Transfer Agreement (MTA). Trpv1^−/− mice (JAX 003770), Trpa1^−/− mice (JAX 006401), nude mice (JAX 001303), Rag1^−/− mice (JAX 002216) and wildtype (WT) mice (C57BL/6J, JAX 000664) were purchased from the Jackson Laboratory (JAX). Advillin^Cre/GCaMP6 mice were generated by crossing GCaMP6f mice (JAX 024339) with Advillin-Cre mice (a gift from Fan Wang’s lab, Duke University). Trpv1^Cre/ ERα^fl/fl mice were generated by crossing Erα^fl/fl mice (JAX 032173) with Trpv1-Cre mice (JAX 017769), which were purchased from the Jackson Laboratory. CD1 mice (Charles River Laboratories) were also used for behavioral tests. Animals were randomly assigned to each group. All animals were maintained at the Duke University Animal Facility. Number of animals used in each experiment was described in Table S3. All animal experiments were approved by the Institutional Animal Care and Use Committees of Duke University.

Luo X., Chen O., Wang Z., Bang S., Ji J., Lee S.H., Huh Y., Furutani K., He Q., Tao X., Ko M.C., Bortsov A., Donnelly C.R., Chen Y., Nackley A., Berta T, & Ji R.R. (2021). IL-23/IL-17A/TRPV1 Axis Produces Mechanical Pain via Macrophage-Sensory Neuron Crosstalk in Female Mice. Neuron, 109(17), 2691-2706.e5.

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Conditional Knockout of FABP5 in Nociceptors

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Mice carrying LoxP-flanked exons 2 and 3 were generated via standard gene-targeting techniques by Cyagen. A 17.5 Kb targeting vector containing a diphtheria toxin (DTA) cassette, neomycin cassette flanked by Frt sites, and exons 2–3 flanked by LoxP sites was electroporated into C57Bl/6 ES cells, which were subsequently selected for blastocyst microinjections. Founders were confirmed as germline-transmitted via crossbreeding with Flp-deleter mice. The F1 progeny were subsequently backcrossed to C57Bl/6 mice and then crossed with Trpv1-Cre mice (Jackson Labs #017769) to ablate FABP5 in TRPV1⁺ nociceptors. Control C57Bl/6J mice were from Jackson Labs.

Bogdan D.M., Studholme K., DiBua A., Gordon C., Kanjiya M.P., Yu M., Puopolo M, & Kaczocha M. (2022). FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain. Scientific Reports, 12, 9241.

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