Anti btla pe

Manufactured by Thermo Fisher Scientific

Anti-BTLA-PE is a fluorescently labeled antibody that binds to the B and T Lymphocyte Attenuator (BTLA) protein. BTLA is an inhibitory receptor expressed on various immune cells, including T cells and B cells. The PE (Phycoerythrin) fluorescent label allows for the detection and analysis of BTLA-expressing cells using flow cytometry or other fluorescence-based techniques.

Automatically generated - may contain errors

Lab products found in correlation

Lsrii flow cytometer, by BD (3 mentions) Anti cd8 po, by BioLegend (2 mentions) Anti 2b4 apc, by Thermo Fisher Scientific (2 mentions) Anti pd 1 apc cy7, by Thermo Fisher Scientific (2 mentions) Anti il 2 fitc, by BD (2 mentions) Anti ifn γ alexa 700, by BD (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) Anti cd3 pb, by BD (1 mentions) Foxp3 staining kit, by BD (1 mentions) Anti cd4 percp, by BD (1 mentions) Anti tnf pe cy7, by BioLegend (1 mentions) Fixation and permeabilization solution, by BD (1 mentions) Golgistop, by BD (1 mentions) Intracellular staining kit, by BD (1 mentions) Anti cd44 fitc, by BD (1 mentions) Anti pd 1 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd4 po, by Thermo Fisher Scientific (1 mentions) Anti thy1.1 percp, by BD (1 mentions) Anti cd8 pb, by Thermo Fisher Scientific (1 mentions) Brefeldin a, by BD (1 mentions)

4 protocols using anti btla pe

Murine Splenocyte Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

2x10⁶ splenocytes from each sample were plated in a 96-well plate. After centrifugation, cells were resuspended and incubated in culture medium (R10) consisting of RPMI 1640 containing 10% FBS (Mediatech, Herndon, VA), 2mM L-glutamine, 0.01 M HEPES buffer, 100mg/ml gentamicin (Mediatech), and 5×10^-5M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO). To test intracellular cytokine, cells were stimulated with 30 mg/ml PMA and 400 ng/ml ionomycin in the presence of GolgiStop (BD Pharmingen) for 4 hours at 37°C.
After incubation and stimulation, cells were surface-stained with anti-CD3-PB (BD), anti-CD4-PerCP (BD), anti-CD8-PO (Biolegend), anti-BTLA-PE, anti-2B4-APC, anti-PD-1-APC-Cy7 (all from eBioscience). Then cells were permeabilized using fixation and permeabilization solution (BD). We used anti-IL-2-FITC (BD), anti-TNF-PE-Cy7 (Biolegend) and anti-IFN-γ-Alexa 700 (BD) for intracellular cytokine staining. In some experiments, cells were stained intracellularly for Ki-67 using the Foxp3 staining kit (BD Pharmingen). Samples were analyzed on an LSRII flow cytometer (BD) and data were analyzed using FlowJo software (FlowJo, LLC) and SPADE (Cytobank.org) (see below).

Xie J., Robertson J.M., Chen C.W., Zhang W., Coopersmith C.M, & Ford M.L. (2018). Pre-existing malignancy results in increased prevalence of distinct populations of CD4+ T cells during sepsis. PLoS ONE, 13(1), e0191065.

+ Open protocol

+ Expand

Antigen-Specific T Cell Immune Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

Groups of mice were sacrificed at the following time points: uninfected (day 0) and post-infection (days 5 and 14). At the indicated time points, spleens were collected from all animals and single cell suspensions were prepared. Cells were stained with anti-CD4-PO (Invitrogen), anti-CD44-FITC and anti-Thy1.1-PerCP (all from BD Pharmingen), anti-CD8-PB, anti-PD-1-FITC, anti-BTLA-PE, and anti-2B4-allophycocyanin (all from eBioscience).
For intracellular cytokine staining, single-cell suspensions of splenocytes were plated in a 96-well plate (1×10⁶ cells per well) in culture medium containing RPMI 1640 containing 10% FBS (Mediatech, Herndon, VA), 2 mM L-glutamine, 0.01 M HEPES buffer, 100 mg/ml gentamicin (Mediatech), and 5×10⁻⁵ M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO). Cells were incubated for 4 h in 10 nM OVA_257-264 (SIINFEKL; Emory University Microchemial Core Facility) and 10 mg/ml brefeldin A (Pharmingen). Following incubation, cells were stained with anti-CD4-PO (Invitrogen), anti-Thy1.1-PerCP (BD Pharmingen), and anti-CD8-PB (eBioscience) and processed using an intracellular staining kit (BD Biosciences) and stained with anti-IFN-γ-Alexa 700 and anti-IL-2-FITC (BD Biosciences). All samples were run on a LSRII flow cytometer (BD Biosciences), and data was analyzed using FlowJo 9.5 Software (Tree Star, San Carlos, CA).

Mittal R., Wagener M., Breed E.R., Liang Z., Yoseph B.P., Burd E.M., Farris AB I.I.I., Coopersmith C.M, & Ford M.L. (2014). Phenotypic T Cell Exhaustion in a Murine Model of Bacterial Infection in the Setting of Pre-Existing Malignancy. PLoS ONE, 9(5), e93523.

+ Open protocol

+ Expand

Analyzing Lymphocyte Responses in LLC Tumor Model

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

LLC cells were seeded in 12-well plates at a density of 1×10⁵ cells/well for 24 h and were transfected with IDO1- or GL2-siRNA. After 24 h of transfection, lymphocytes isolated from the spleen of LLC-bearing C57BL/6 mice (33 (link)). Briefly, spleens (~1 cm in length and 30 mm in width) were placed on a 40 µm Falcon Cell Strainer (VWR International, LLC.) and gently squashed with a plunger. The cell suspension was collected to centrifuge at 4°C, 250 g for 5 min, then further isolated using ACK Lysing Buffer (Beijing Solarbio Science & Technology Co., Ltd.) to lyse red blood cells. Lymphocytes were added to the LLC cells at a density of 5×10⁵ cells/well and were cultured at 37°C with 5% CO₂ for 48 h. Lymphocytes were subsequently collected and stained with anti-CD4-FITC (cat. no. 553047; 1:200; BD Pharmingen; BD Biosciences), anti-CD8-PE (cat. no. 12-0081-82; 1:200; eBioscience; Thermo Fisher Scientific, Inc.), anti-PD-1-APC (cat. no. 17-9985-82; 1:200; eBioscience; Thermo Fisher Scientific, Inc.) and anti-BTLA-PE (Cat. no. 12-5950-82; 1:200; eBioscience; Thermo Fisher Scientific, Inc.), incubated at 4°C in dark for 30 min, then detected using flow cytometry (BD FACSCanto II; BD Biosciences). The data were analyzed suing FlowJo version 10 software (Becton, Dickinson and Company).

Shang K., Wang Z., Hu Y., Huang Y., Yuan K, & Yu Y. (2020). Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion. Oncology Letters, 19(6), 3827-3838.

+ Open protocol

+ Expand

Phenotypic Characterization of Splenic T Cells in Sepsis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were sacrificed and spleens were harvested 24-hours post CLP. Splenocytes were stained with anti-CD3-Alexa 700 (BD), anti-CD4-PB (BD), anti-CD8-PO and anti-CD44-PerCP (Biolegend). Cells were also surface stained with anti-CD25-FITC (Biolegend), anti-CD69-PE (Biolegend), anti-CD62L-PE Cy7 (BD), and anti-BTLA-PE, anti-2B4-APC, anti-PD-1-APC-Cy7, anti-LAG-3-FITC (all from eBioscience) for phenotypic analysis. An LSR II flow cytometer (BD Biosciences) was used to run all samples. Accucheck Counting Beads (Thermo Fisher Scientific) were added during staining to calculate the absolute number of T cells per spleen. Flow data were analyzed using FlowJo software (FlowJo, LLC) and SPADE (Cytobank.org) (see below).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!