Data on baseline characteristics, including age, sex, body mass index (BMI), and smoking status, were collected. Total, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol levels and triglyceride levels were measured using colorimetric assays (Hitachi LST008, Automatic Clinical Chemistry Analyzer, Hitachi, Naka, Japan). Non-HDL and remnant cholesterol levels were calculated by subtracting HDL from total cholesterol levels and by subtracting HDL and LDL from total cholesterol levels, respectively [45 (link)]. The definition of metabolic syndrome is shown in the Supplementary Materials , and other metabolic traits are shown in Supplementary Table S7 and as previously reported [41 (link),42 (link)].
Lst008
Manufactured by Hitachi
Sourced in Japan
The LST008 is a laboratory equipment product manufactured by Hitachi. It is designed to perform specific tasks within a laboratory setting. The core function of this product is to provide the necessary capabilities for laboratory operations, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
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8 protocols using lst008
1
Metabolic Trait Measurements in Cohort
2
Biomarker Detection Protocol
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CysC, sNGAL/uNGAL and sCr were detected using corresponding kits provided by Maccura Biotechnology Company (Chengdu, China). The detections were performed on the LST008 automatic biochemical analyzer (Hitachi, Japan). The uCr was detected using kits provided by Biostec Company (Chongqing, China) on the BioSystems A25 fully automatic specific protein analyzer (BioSystems, Spain).
3
Cushing's Syndrome Biomarker Analysis
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The clinical data of the Cushing’s syndrome patients were obtained from their medical records. Fasting blood samples were obtained after fasting for 12 h and were kept at -80°C. Fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) concentrations were quantified using an automatic biochemical analyzer (LST-008; Hitachi, Japan). Glycosylated Hemoglobin Type A1C (HbA1c) was detected using high-performance liquid chromatography (HA8180 automatic glycosylated hemoglobin analyzer). Cortisol and adrenocorticotropic hormone (ACTH) were measured using an electrochemiluminescence immunoassay (Roche Cobas 6000 automatic electrochemiluminescence analyzer), and 24 h UFC was detected using liquid chromatography-tandem mass spectrometry (Changsha Kingmed Center for Clinical Laboratory, China).
4
Comprehensive Lifestyle and Health Profiling
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Sociodemographic (age, sex, ethnicity), lifestyle (exercise, alcohol consumption, cigarette smoking) and disease (hypertension and diabetes) data were gathered from the TWB questionnaires. Using colorimetric assays (Hitachi LST008, Automatic Clinical Chemistry Analyzer, Hitachi, Naka, Japan), individual lipid levels, including HDL cholesterol, triglycerides (TG), and non-HDL cholesterol were measured from blood samples collected at TWB assessment centers.
In this study, exercise habits were divided into three categories: no exercise, aerobic exercise, and resistance exercise. Subjects who regularly exercised for at least 30 min a day, three times a week, were considered regular exercisers. Ball games and weight training were included in the study as forms of resistance exercise. Aerobic exercise included Chinese martial arts, brisk walking, Taijiquan, hiking, jogging, basketball, rope jumping, gymnastics, yoga, qigong, swimming, biking, table tennis, tennis, soccer, badminton, aerobic dance, hula hooping, golf, and ballroom dance.
In this study, exercise habits were divided into three categories: no exercise, aerobic exercise, and resistance exercise. Subjects who regularly exercised for at least 30 min a day, three times a week, were considered regular exercisers. Ball games and weight training were included in the study as forms of resistance exercise. Aerobic exercise included Chinese martial arts, brisk walking, Taijiquan, hiking, jogging, basketball, rope jumping, gymnastics, yoga, qigong, swimming, biking, table tennis, tennis, soccer, badminton, aerobic dance, hula hooping, golf, and ballroom dance.
5
Metabolic Syndrome Diagnostic Criteria
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Metabolic syndrome was defined according to the criteria of the Ministry of Health and Welfare in Taiwan. Participants were classified as having metabolic syndrome if they met more than three of the following conditions: (1) waist circumference ≥ 90 cm for men and ≥ 80 cm for women; (2) systolic blood pressure (SBP) ≥ 130 mmHg or diastolic blood pressure (DBP) ≥ 85mmHg; (3) fasting blood glucose ≥ 100mg/dl; (4) high-density lipoprotein < 40 mg/dl for men and < 50 mg/dl for women; and (5) triglyceride ≥ 150 mg/dl. The tools used to assess the above variables were 1) measuring tape, for waist circumference; 2) blood pressure monitor (OMRON HEM7320, HEM7210), for blood pressure; 3) clinical analyzer (Hitachi LST008), for fasting blood glucose, HDL, and triglycerides. The tests were performed at the Chang Gung Memorial Hospital, Linkou, Taiwan following the manufacturers' instructions.
6
Lipid and Adipokine Biomarker Measurement
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Blood samples were collected at the outpatient clinics after the subjects had fasted for at least 8 hours. The collected samples were frozen immediately and stored at −80 °C until required. Total cholesterol concentrations as well as triglyceride, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels were measured by an automated enzymatic colorimetric assay (Hitachi LST 008, Tokyo, Japan). Apolipoprotein A1 (Apo-A1) and Apo-B levels were measured using an immunoturbidimetric assay with an automatic biochemical analyzer. Serum adiponectin and leptin levels were measured by enzyme-linked immunosorbent assay (ELISA) (Tecan Sunrise, Mannedorf, Switzerland).
7
Canine Blood and Tissue Analysis
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Dogs’ peripheral blood collected at preoperative 1 day and postoperative 7, 28, and 90 days was detected by an automatic blood cell analyzer (LH750, Beckman Coulter, USA), including hemoglobin (Hb), leukocyte (WBC), and platelet (PLT). The centrifugal blood serum was detected by an automatic biochemical analyzer (LST008, Hitachi, Japan), including alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ABL), urea nitrogen (BUN), creatinine (Cr), magnesium ion, calcium ion, potassium ion. The detection of magnesium, aluminum, zinc, and manganese in the heart, liver, kidney, liver, brain, spleen, and skeletal muscle were analyzed by an inductively coupled plasma mass spectrometer (DRC-II, PerkinElmer, USA) and a plasma emission spectrometer (iCap6000, Thermo Fisher Scientific, USA).
8
Lipid Profiling in Baseline Characteristics
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In addition to baseline characteristics (i.e., age, sex, current smoking, and body mass index), we measured the lipid profiles (i.e., total cholesterol, LDL and HDL cholesterol, and triglyceride levels) by using colorimetric assays (Hitachi LST008, Automatic Clinical Chemistry Analyzer, Hitachi, Naka, Japan). Non-HDL and remnant cholesterol levels were calculated as total cholesterol minus HDL cholesterol levels and total cholesterol minus HDL cholesterol minus LDL cholesterol, respectively [56 (link)].
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