Myrosinase, sinigrin, and rapeseed standard reference material of known glucosinolate composition were obtained from Sigma Aldrich (Steinheim, Germany). Glucoraphanin and glucotropaeolin were obtained from Phytoplan (Heidelberg, Germany), while sinalbin was isolated from the seeds of Sinapis alba L., and glucoerucin from Eruca vesicaria (L.) Cav. All other chemicals and reagents were of an analytical grade. Cancer cell lines (human bladder cancer cell line T24 and TCCSUP) were cultured in a humidified atmosphere with 5% CO2 at 37 °C, in a Dulbecco’s modified Eagle’s medium (DMEM, EuroClone, Milano, Italy) containing 4.5 g/L glucose, 10% fetal bovine serum (FBS), and 1% antibiotics (Penicillin Streptomycin, EuroClone, Milano, Italy).
Dulbecco s modified eagle s medium dmem
Manufactured by Euroclone
Sourced in Italy, United States, United Kingdom
Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium that provides a balanced salt solution and essential nutrients to support the growth and maintenance of various cell types. It is a widely used medium in biomedical research, cell and tissue culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Euroclone (8 mentions)
L glutamine, by Euroclone (7 mentions)
Penicillin, by Euroclone (5 mentions)
Streptomycin, by Euroclone (5 mentions)
Penicillin streptomycin, by Euroclone (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Gentamicin, by Merck Group (2 mentions)
Fetal calf serum (fcs), by Euroclone (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Glucoraphanin, by Phytoplan (1 mentions)
Sinigrin, by Merck Group (1 mentions)
Myrosinase, by Merck Group (1 mentions)
Sinalbin, by Phytoplan (1 mentions)
Glucotropaeolin, by Phytoplan (1 mentions)
Glucoerucin, by Phytoplan (1 mentions)
Rpmi 1640 medium, by Euroclone (1 mentions)
Amphotericin, by Euroclone (1 mentions)
Amphotericin b, by Euroclone (1 mentions)
Nhdf ad human dermal fibroblasts, by Lonza (1 mentions)
20 protocols using dulbecco s modified eagle s medium dmem
1
Glucosinolate Compounds in Cancer Cell Lines
2
Culturing SKNBE2 and SHSY5Y Neuroblastoma Cells
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SKNBE2 and SHSY5Y neuroblastoma cells were provided by the cell bank of the National Institute of Cancer Research (IST) Genoa, Italy and obtained from ECACC.
SKNBE2 were maintained in RPMI 1640 medium (EuroClone, Devon, UK), 10% FBS (GIBCO, S. Giuliano Milanese, Milan, Italy), 2 mM L-glutamine (EuroClone), 100 U/mL penicillin–streptomycin (EuroClone). SHSY5Y cells were cultured in Dulbecco’s modified Eagles medium (DMEM) (EuroClone), 10% FBS (GIBCO), 2 mM L-glutamine (EuroClone), and 100 U/mL penicillin-streptomycin (EuroClone).
SKNBE2 were maintained in RPMI 1640 medium (EuroClone, Devon, UK), 10% FBS (GIBCO, S. Giuliano Milanese, Milan, Italy), 2 mM L-glutamine (EuroClone), 100 U/mL penicillin–streptomycin (EuroClone). SHSY5Y cells were cultured in Dulbecco’s modified Eagles medium (DMEM) (EuroClone), 10% FBS (GIBCO), 2 mM L-glutamine (EuroClone), and 100 U/mL penicillin-streptomycin (EuroClone).
3
Fabrication and Characterization of PBS Biomaterials
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Poly (1,4-butylene succinate) extended with 1,6-diisocyanatohexane (Tm 120 °C) (PBS), 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Aldrich Milan, Italy. This merchant specifies only the Tm for PBS, but Fabbri et al. performed a GPC analysis to evaluate the molecular weight (81.2 kDa) [21 (link)].
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin, l-glutamine, penicillin, streptomycin, and amphotericin were purchased from Euroclone group (Milan, Italy).
Porcine bile was extracted from pigs at Istituto Zooprofilattico della Sicilia “A. Mirri,” Palermo, Italy accordingly with European rules on animal experiments.
Human blood was extracted from volunteers upon informed consent and isolated at the University of Palermo, Palermo, Italy.
NHDF-Ad-Human Dermal Fibroblasts, Adult were obtained from Lonza bioscience and used after 9 doublings. The cell line was grown in a minimum essential medium [Dulbecco’s modified Eagle’s medium (DMEM)] supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM l-glutamine, 100 um/mL penicillin, 100 μg/mL streptomycin, and 2.5 μg/mL amphotericin B (all reagents were from Euroclone, Milan, Italy) under standard conditions (95% relative humidity, 5% CO2, 37 °C).
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin, l-glutamine, penicillin, streptomycin, and amphotericin were purchased from Euroclone group (Milan, Italy).
Porcine bile was extracted from pigs at Istituto Zooprofilattico della Sicilia “A. Mirri,” Palermo, Italy accordingly with European rules on animal experiments.
Human blood was extracted from volunteers upon informed consent and isolated at the University of Palermo, Palermo, Italy.
NHDF-Ad-Human Dermal Fibroblasts, Adult were obtained from Lonza bioscience and used after 9 doublings. The cell line was grown in a minimum essential medium [Dulbecco’s modified Eagle’s medium (DMEM)] supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM l-glutamine, 100 um/mL penicillin, 100 μg/mL streptomycin, and 2.5 μg/mL amphotericin B (all reagents were from Euroclone, Milan, Italy) under standard conditions (95% relative humidity, 5% CO2, 37 °C).
4
Cell Viability Assay Protocol
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Thiazolyl blue tetrazolium bromide (MTT); isopropanol; streptomycin/penicillin; hydrogen peroxide (H2O2); gentamicin; phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich (Milan, Italy). Dulbecco’s modified Eagle’s medium (DMEM) and Fetal Bovine Serum (FBS) were purchased from Euroclone S.p.A. (Pero, Milan); dimethyl sulfoxide (DMSO).
5
Isolation and Expansion of Bone Marrow Mesenchymal Stem Cells
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BMSCs were isolated from the iliac crest bone marrow aspirates of healthy donors after informed consent was obtained. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by Nanjing Drum Tower Hospital’s institutional review board. Bone marrow mononuclear cells were isolated by Percoll (Solarbio, USA), Dulbecco’s modified Eagle’s medium (DMEM) (Euroclone, USA) with 1,000 mg/mL glucose and L-glutamine, centrifuged and plated at a density of 1,000 cells/cm2. BMSCs were cultured in L-DMEM/F12 (GIBCO, USA) with 15% fetal bovine serum (GIBCO, USA) at 37 °C with 5% CO2. Four days later, non-adherent cells were removed carefully, and the culture medium was refreshed. When primary cultures became almost confluent, the culture was treated with 0.5 mL of 0.25% trypsin containing 0.02% mmol/L ethylenediamine tetraacetic acid (GIBCO, USA) for 4 min at room temperature (25 °C). A purified population of BMSCs was obtained 1 week after the initiation of culture.
6
Molecular Mechanism Study Protocol
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EGCG, l -glutamine, penicillin-streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), 1,4-diazabicyclo(2.2.2)ctane (DABCO), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) were all purchased by Sigma-Aldrich, St. Louis, MO, USA. B27 was provided by Thermo-Fisher, Waltham, MA, USA. 6-OH-11-O-hydroxyphenanthrene (IIF) (pat. WIPO W0 00/17143) was provided by K. Ammar, Houston, TX USA. E-MEM, Dulbecco’s modified Eagle’s medium (D-MEM), D-MEM/nutrient mixture F-12 (DMEM/F12), and FBS were purchased by Euroclone, Milan, Italy. Formalin (40%) was from Carlo Erba, Milan, Italy. Antibodies: anti-RARα and anti-RXRγ (Tema Ricerca, Bologna, Italy), anti-EGFR (Thermo Scientific, Waltham, MA, USA), anti-p1068EGFR (Novex, Life Technologies, Carlsbad, CA, USA), anti-Bcl-2 (Sigma-Aldrich, St. Louis, MO, USA), anti-Bax (Applied Biosystem, Monza, Italy) anti-PARP (Santa Cruz Biotechnology, Dallas, TX, USA), anti-COX-2 (Sigma-Aldrich, St. Louis, MO, USA), anti-N-MYC, anti MMP-2, MMP-9, and anti-TIMP-1 (all from Santa Cruz Biotechnology, Dallas, TX, USA), anti-β-tubulin (Sigma-Aldrich, St. Louis, MO, USA), anti-rabbit, and anti-mouse peroxidase conjugated antibodies (GE Healthcare, Milan, Italy).
7
Culturing Murine and Human Cell Lines
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The murine monocyte/macrophage cell line (J774A.1), murine fibrosarcoma cells (WEHI-164), and human epithelial kidney cells (HEK-293) were obtained from American Tissue Culture Collection (ATCC). Dulbecco's modified Eagle's medium (DMEM), penicillin/streptomycin HEPES, glutamine, fetal calf serum (FCS), and horse serum were from Euroclone (Euroclone-Celbio, Pero, Milan, Italy). J774.A1 were grown in adhesion on Petri dishes and maintained at 37°C as previously described [20 (link)]. WEHI-164 and HEK-293 were maintained in adhesion on Petri dishes with DMEM supplemented with 10% heat-inactivated FCS, 25 mM HEPES, 100 u/mL penicillin, and 100 μg/mL streptomycin.
8
Cell Culture Conditions and Maintenance
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The cell lines used were described in our previous study [21 (link)]. CMT-U309 and CMT-U27 cells were cultured in Roswell Park Memorial Institute (RPMI) medium (Euroclone, Milan, Italy); P114 cells were maintained in Dulbecco’s Modified Eagle’s medium/Nutrient Mixture F-12 Ham (DMEM/F12) (Euroclone); and CF33 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Euroclone). All cell lines were supplemented with 10% fetal bovine serum (Euroclone), 100 IU/mL penicillin/100 μg/mL streptomycin (Euroclone), and 2 mM L-glutamine (Euroclone), and grown in an atmosphere of 5% CO2 and 95% humidity at 37 °C. All cell lines were routinely tested for mycoplasma contamination.
9
Fibroblast Culture and CCCP-Induced Stress
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Primary skin fibroblast cell lines were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (Euroclone) supplemented with 15% (v/v) fetal bovine serum (FBS) (Euroclone), 100 U/ml penicillin, 100 μg/ml streptomycin (Euroclone), and 2 mM L-glutamine (Euroclone) and maintained at 37°C under humidified conditions and 5% CO2. Cells were subcultured twice weekly, detached with Accutase (Euroclone), and centrifuged at 500 × g for 10 min at 25°C. Cells were used at passage number lower than 13.
Fibroblast cells were seeded at a density of 5 × 105 per 75 cm2 flask for 24 h before treatments. Cells were then exposed to carbonyl cyanide m-chlorophenylhydrazone (CCCP) dissolved in dimethyl sulfoxide (DMSO) at a concentration of 60 μM or to an equal volume of DMSO alone, for 24 h.
Fibroblast cells were seeded at a density of 5 × 105 per 75 cm2 flask for 24 h before treatments. Cells were then exposed to carbonyl cyanide m-chlorophenylhydrazone (CCCP) dissolved in dimethyl sulfoxide (DMSO) at a concentration of 60 μM or to an equal volume of DMSO alone, for 24 h.
10
Electrospun Polycaprolactone Nanofibers for Drug Delivery
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Polycaprolactone (PCL, Mw = 80000),
dichloromethane (DCM), N,N-dimethylformamide
(DMF), methanol, and chloroform
were purchased from Sigma-Aldrich (St. Louis). Glass syringes (with
an inner diameter of 14.6 mm), three-layers coaxial needle, and coaxial
kit were acquired from Linari NanoTech (Pisa, Italy). The KDS-100-CE
syringe pump was purchased from KD Scientific (Holliston, MA). Rifampicin
was acquired from EMD Millipore Corp. The D-ES30PN-20W potential generator
was purchased from Gamma High Voltage Research Inc. (Ormond Beach,
FL). Recombinant Trypsin–EDTA 1X, penicillin/streptomycin 100X,l -glutamine 100X, fetal bovine serum (FBS), and Dulbecco’s
modified Eagle’s medium (DMEM) were purchased from Euroclone
(Milan, Italy).
dichloromethane (DCM), N,N-dimethylformamide
(DMF), methanol, and chloroform
were purchased from Sigma-Aldrich (St. Louis). Glass syringes (with
an inner diameter of 14.6 mm), three-layers coaxial needle, and coaxial
kit were acquired from Linari NanoTech (Pisa, Italy). The KDS-100-CE
syringe pump was purchased from KD Scientific (Holliston, MA). Rifampicin
was acquired from EMD Millipore Corp. The D-ES30PN-20W potential generator
was purchased from Gamma High Voltage Research Inc. (Ormond Beach,
FL). Recombinant Trypsin–EDTA 1X, penicillin/streptomycin 100X,
modified Eagle’s medium (DMEM) were purchased from Euroclone
(Milan, Italy).
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