Agilent’s Inorganic Anion Test Mixture stock (1000 ppm fluoride, chloride, bromide, nitrite, nitrate, sulfate, and 2000 ppm phosphate) was diluted 1:10 with deionized (DI) water obtained from EMD Millipore Elix10 Water Purification System (Darmstadt, Germany) and used as a standard. Stocks of the following inorganic anions (from their sodium or potassium salts) and organic acids (i.e., lactic acid) were made at 1000 ppm: acetate, arsenate, azide, bicarbonate/carbonate, bromide, chloride, fluoride, lactate, nitrate, nitrite, phosphate, silicate, sulfate, and thiosulfate. Each anion and organic acid was diluted from its stock solution to make a 20 ppm standard mixture and was filtered using a 0.22 μm PES syringe filter (Argos Technologies, Elgin, IL, USA). The running buffer/BGE was Agilent’s proprietary Basic Anion Buffer (pH 12.0) obtained from their Forensic Anion Solution Kit. The BGE was degassed for 10 min in a TA Instruments degassing station (New Castle, DE, USA) using 2 mL glass CE vials from Agilent Technologies prior to runs.
Basic anion buffer
Manufactured by Agilent Technologies
The Basic Anion Buffer is a laboratory reagent used to maintain a specific pH range in aqueous solutions. It is designed to facilitate the analysis and separation of anionic analytes in various analytical techniques.
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2 protocols using basic anion buffer
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Inorganic Anion Mixture Characterization
2
Inorganic Anion Mixture Characterization
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Agilent’s Inorganic Anion Test Mixture stock (1000 ppm fluoride, chloride, bromide, nitrite, nitrate, sulfate, and 2000 ppm phosphate) was diluted 1:10 with deionized (DI) water obtained from EMD Millipore Elix10 Water Purification System (Darmstadt, Germany) and used as a standard. Stocks of the following inorganic anions (from their sodium or potassium salts) and organic acids (i.e., lactic acid) were made at 1000 ppm: acetate, arsenate, azide, bicarbonate/carbonate, bromide, chloride, fluoride, lactate, nitrate, nitrite, phosphate, silicate, sulfate, and thiosulfate. Each anion and organic acid was diluted from its stock solution to make a 20 ppm standard mixture and was filtered using a 0.22 μm PES syringe filter (Argos Technologies, Elgin, IL, USA). The running buffer/BGE was Agilent’s proprietary Basic Anion Buffer (pH 12.0) obtained from their Forensic Anion Solution Kit. The BGE was degassed for 10 min in a TA Instruments degassing station (New Castle, DE, USA) using 2 mL glass CE vials from Agilent Technologies prior to runs.
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