FTIR spectra were determined using a Nexus model 670 spectrophotometer using the Omnic software package. TGA was performed using a TA Instruments Q50 under nitrogen at a scan rate of 15 °C/min from 50 to 800 °C X-ray diffraction was measured using Cu Kα radiation on an Inel CPS 120 position-sensitive detector with a XRG 3000 generator using a 20 min collection time. ζ-Potentials and particle sizes were measured in water using a Brookhaven Instruments Corporation phase analysis light scattering (PALS) ζ-potential analyzer. All values are an average often 10 s scans. XPS spectra were recorded and processed using a Physical Electronics Versaprobe II X-ray photoelectron spectrometer. Raman spectra were recorded on a Horiba Lab Ram equipped with a 533 nm YAG laser using LabSpec 5 processing software. SEM was performed on gold-coated samples using a JEOL 6010LA microscope with a tungsten source and an SEI detector. TEM was performed using a FEI Tecnai G2 Spirit TWIN microscope. Samples were prepared on lacey carbon or copper grids.
Tecnai g2 spirit twin microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tecnai G2 Spirit Twin microscope is a high-performance transmission electron microscope (TEM) designed for advanced imaging and analysis applications. It features a twin-lens system that allows for both low and high magnification imaging. The microscope is equipped with a high-resolution electron source and advanced optics to deliver clear and detailed images of a wide range of samples.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 2000, by Thermo Fisher Scientific (2 mentions)
Versaprobe 2 x ray photoelectron spectrometer, by Physical Electronics (1 mentions)
Carbon coated copper grid, by Ted Pella (1 mentions)
Whatman paper, by Cytiva (1 mentions)
Em afs2 system, by Leica (1 mentions)
Em pact2, by Leica (1 mentions)
6 protocols using tecnai g2 spirit twin microscope
1
Comprehensive Nanomaterial Characterization Protocol
2
Negative Staining for Protein Complexes
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MB or NIR-Alb NP complex was applied to glow-discharged carbon-coated copper grids (200 mesh, Ted Pella, USA). After allowing the sample to absorb for 2 mins and blotting off buffer solution onto Whatman paper, then the sample on the grids was stained with 2% (w/v) of uranyl acetate (UrAc) for 1 min. Then, it was blotted off UrAc. These results were recorded with the Tecnai G2 Spirit Twin microscope (FEI, USA) at an acceleration voltage of 120 kV.
3
Visualizing Bacteriophage Morphology
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Purified bacteriophage suspension (over 1.0 × 109 PFU/ml) was applied to glow-discharged carbon-coated copper grids. After allowing the sample to absorb for 2 min, buffer solution was blotted off onto a Whatman paper and the sample on the grids were stained with 2% (w/v) uranyl acetate (UrAc) for 1 min. UrAc was blotted off. These results were recorded with the Tecnai G2 Spirit Twin microscope (FEI, United States) at an acceleration voltage of 120 kV.
4
Algae Ultrastructural Analysis by Electron Microscopy
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Algal cells grown after 6 days in the time-course experiments were harvested and fixed using a high pressure freezing system (EM PACT2, Leica) at 2000–2050 bar. Freeze substitution in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate was conducted using a Leica EM AFS2 system. Samples were maintained at −85°C for 3 days, followed by −60, −20, and 0°C for 1 day each; finally, the samples were kept at room temperature. After rinsing with acetone for 12 h twice, the samples were infiltrated and embedded in Spurr’s resin or LR gold resin. Ultrathin sections were stained with uranyl acetate (5%) in 50% methanol for 10 min and then stained with lead citrate (0.5%) for 4 min. Sections were examined using a Tecnai G2 Spirit TWIN microscope (FEI Company).
5
Ultrastructure Visualization via TEM
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Samples were cultured and collected under the same conditions as for RNA-Seq samples, fixed in 2% glutaraldehyde for 2 h, then subjected for 1.5 h to second fixation with 1.5% potassium permanganate solution, rinsed twice (7 min each time) with PIPES 0.1 M, washed by ethanol gradient (30, 50, 70, 85, 95%) elution, washed three times (10 min each time) with 100% ethanol, soaked in acetone/ Spurr resin (ratios 3:1, 1:1,1:3; 2 h each time), and embedded overnight in Spurr resin. Images were recorded by TEM using a Tecnai G2 Spirit Twin microscope (FEI) at acceleration voltage 120 kV.
6
Biomolecular Extraction and Analysis
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The DNA, RNA, Protein content was prepared using extraction kit (Omega, US), and determined by Nano drop2000 (Thermo), the fatty acid content was determined the same as Miao et al 2010 [23] .
Total RNA puri cation and reverse transcription (ratios 3:1, 1:1,1:3; 2 h each time), and embedded overnight in Spurr resin. Images were recorded by TEM using a Tecnai G2 Spirit Twin microscope (FEI) at acceleration voltage 120 kV.
Total RNA puri cation and reverse transcription (ratios 3:1, 1:1,1:3; 2 h each time), and embedded overnight in Spurr resin. Images were recorded by TEM using a Tecnai G2 Spirit Twin microscope (FEI) at acceleration voltage 120 kV.
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