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Vi cell cell analyzer

Manufactured by Beckman Coulter
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Sourced in Germany, United States

The Vi-CELL cell analyzer is a compact, automated instrument that performs cell counting and viability analysis. It utilizes trypan blue dye exclusion and digital image processing to accurately determine the total cell count and percentage of viable cells in a sample.

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Vi-Cell (149 citations) Vi-CELL Cell Viability Analyzer (87 citations) Vi-CELL XR Analyzer (19 citations) Vi-CELL BLU Cell Viability Analyzer (9 citations) Vi-Cell Automated Cell Viability Analyzer (4 citations) Vi-Cell MetaFLEX Bioanalyte analyzer (3 citations) Vi-CELL™ Cell Counter for Cell Viability Analyzer (3 citations) Vi-CELL (12-Sample Carousel) Cell Viability Analyzer (3 citations) Vi-Cell automated analyzer (2 citations) Vi-CELL MetaFLEX™ analyzer (2 citations)

9 protocols using vi cell cell analyzer

1

Lung Cancer Cell Viability Assay

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Lung cancer cells were plated into 6-well plates and treated the next day for the designated period of time. Cells were trypsinized and the number of live and dead cells were determined using the Vi-CELL cell analyzer (Beckman Coulter), which analyzed 500 cells per sample and utilized trypan blue dye exclusion to determine dead cells.
NeMoyer R., Mondal A., Vora M., Langenfeld E., Glover D., Scott M., Lairson L., Rongo C., Augeri D.J., Peng Y., Jabbour S.K, & Langenfeld J. (2019). Targeting bone morphogenetic protein receptor 2 sensitizes lung cancer cells to TRAIL by increasing cytosolic Smac/DIABLO and the downregulation of X-linked inhibitor of apoptosis protein. Cell Communication and Signaling : CCS, 17, 150.
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2

Cell Viability Assessment by Automated Counting

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Cells were plated in duplicate into 6-well plates and treated the next day for the designated time period. Cell counts were determined using the automated cell counter Vi-CELL cell analyzer (Beckman Coulter). Approximately 500 cells per sample were analyzed and trypan blue dye exclusion determined the number of dead cells. The experiment was replicated three times in our laboratory.
Mondal A., Roberge J., Gilleran J., Peng Y., Jia D., Akel M., Patel Y., Zoltowski H., Doraiswamy A, & Langenfeld J. (2022). Bone morphogenetic protein inhibitors and mitochondria targeting agents synergistically induce apoptosis-inducing factor (AIF) caspase-independent cell death in lung cancer cells. Cell Communication and Signaling : CCS, 20, 99.
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3

Cell Viability Determination Protocol

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450,000 cells per well were plated into 6-well plates and grown overnight. Next day, cells were treated for the designated period. An automated cell counter Vi-CELL cell analyzer (Beckman Coulter) was used to determine the cell viability. The Vi-CELL cell analyzer uses trypan blue dye in approximately 500 cells per sample to determine the number of dead cells.
Mondal A., NeMoyer R., Vora M., Napoli L., Syed Z., Langenfeld E., Jia D., Peng Y., Gilleran J., Roberge J., Rongo C., Jabbour S.K, & Langenfeld J. (2021). Bone morphogenetic protein receptor 2 inhibition destabilizes microtubules promoting the activation of lysosomes and cell death of lung cancer cells. Cell Communication and Signaling : CCS, 19, 97.
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4

Impact of Temperature on Cryopreserved Cell Viability

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The influence of temperature changes during deep temperature storage on cell viability and cell recovery was determined by using the trypan blue dye exclusion test. The measurement was performed using an automated cell analyzer (ViCell cell analyzer; Beckman Coulter, Krefeld, Germany). The cell viability and cell recovery from three samples per cycle condition per donor were measured three times immediately after thawing as well as after overnight culture.

Viability:
Recovery (%) directly after thawing:

Recovery (%) after overnight culture:
Angel S., von Briesen H., Oh Y.J., Baller M.K., Zimmermann H, & Germann A. (2016). Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality. Biopreservation and Biobanking, 14(6), 539-547.
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5

Cell Viability Quantification in Glioma

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For SD2 cell, 300,000 cells/well and for U87 cell 400,000 cells/well were seeded in duplicate into 6-well plates and grown overnight at 37° incubator with 5% CO2. The cells were then treated for the designated time period. After the treatment, SD2 cells were harvested with accutase and U87 cells were harvested with 0.05% trypsin. An automated Vi-CELL cell analyzer (Beckman Coulter, USA) was used to determine the percent dead cells and total number of live cells in each treatment group. Each experiment was replicated at least 4 times in our laboratory.
Kaye J., Mondal A., Foty R., Jia D, & Langenfeld J. (2022). Bone morphogenetic protein receptor inhibitors suppress the growth of glioblastoma cells. Molecular and cellular biochemistry, 477(5), 1583-1595.
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6

Cell Viability Analysis Protocols

Cited 1 time
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Cells were plated in duplicate into 6-well plates and treated the next day for the designated period of time. Cell counts were determined using the automated cell counter Vi-CELL cell analyzer (Beckman Coulter) as previously reported15 (link). Approximately 500 cells per sample were analyzed and trypan blue dye exclusion determined number of dead cells. Cells treated with Ym155, Atpenin, and Phenformin were treated for 48 h and cells treated with cisplatin were treated for 5 days. The cell count experiments were performed at least 2 times.
Mondal A., Jia D., Bhatt V., Akel M., Roberge J., Guo J.Y, & Langenfeld J. (2022). Ym155 localizes to the mitochondria leading to mitochondria dysfunction and activation of AMPK that inhibits BMP signaling in lung cancer cells. Scientific Reports, 12, 13135.
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7

Cell Viability and Cytotoxicity Assay

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Cells were plated in duplicate into 6-well plates and treated the next day for the designated period of time. Cell counts were determined using the automated cell counter Vi-CELL cell analyzer (Beckman Coulter).
Approximately 500 cells per sample were analyzed and trypan blue dye exclusion determined number of dead cells. The experiment was replicated three times in our laboratory.
Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce Apoptosis-Inducing Factor (AIF) caspase independent cell death.
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8

Cell Viability and Cytotoxicity Assay

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Cells were plated in duplicate into 6-well plates and treated the next day for the designated period of time. Cell counts were determined using the automated cell counter Vi-CELL cell analyzer (Beckman Coulter).
Approximately 500 cells per sample were analyzed and trypan blue dye exclusion determined number of dead cells. The experiment was replicated three times in our laboratory.
Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce Apoptosis-Inducing Factor (AIF) caspase independent cell death.
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9

Cell Viability and Cytotoxicity Assay

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Cells were plated in duplicate into 6-well plates and treated the next day for the designated period of time. Cell counts were determined using the automated cell counter Vi-CELL cell analyzer (Beckman Coulter).
Approximately 500 cells per sample were analyzed and trypan blue dye exclusion determined number of dead cells. The experiment was replicated three times in our laboratory.
Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce AIF-caspase independent cell death.
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