Cells were collected and subjected to ChIP according to the manufacturer's instructions (HighCell#ChIP kit, Diagenode, NJ) using anti‐PARP1 monoclonal antibody (Cell Signaling), anti‐mouse CHD1L polyclonal antibody (LTK BioLaboratories), anti‐histone H3 monoclonal antibody (ChIP Formulated; Cell Signaling), and anti‐di‐methyl‐histone H3 (Lys4) monoclonal antibody (Cell Signaling). DNA eluted from precipitated complexes was analyzed by qPCR. qPCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, CA) according to the manufacturer's instructions. Signals were detected with LC480 (Roche, Mannheim, Germany). Primer sequences are listed in Supporting Information Materials and Methods.
Anti parp1 monoclonal antibody
Manufactured by Cell Signaling Technology
The Anti‐PARP1 monoclonal antibody is a laboratory reagent used for the detection and analysis of PARP1 protein in various experimental systems. The antibody specifically recognizes and binds to the PARP1 protein, enabling its identification and quantification in samples. This antibody is a valuable tool for researchers studying PARP1 and its role in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Highcell chip kit, by Diagenode (1 mentions)
Lc480, by Roche (1 mentions)
Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Protein a mag sepharose, by GE Healthcare (1 mentions)
Anti histone h3 monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti tubulin monoclonal antibody, by Merck Group (1 mentions)
Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti parp1 monoclonal antibody
1
ChIP-qPCR Protocol for Epigenetic Analysis
2
Protein Interaction Analysis by Western Blotting
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Western blotting (WB) analysis was performed as previously described 27 . Immunoprecipitation assays were performed using Protein A Mag Sepharose (GE Healthcare, NJ). The primary antibodies for WB and immunoprecipitation were anti‐OCT4 monoclonal antibody (Cell Signaling, MA), anti‐PARP1 monoclonal antibody (Cell Signaling), anti‐Poly(ADP ribose) monoclonal antibody (Enzo, Lausen, Switzerland), anti‐mouse CHD1L polyclonal antibody against the peptide GRDYSKEPSKEDRKSFEQL (LTK BioLaboratories, Taipei, Taiwan), anti‐human CHD1L monoclonal antibody (Abcam, Inc., Cambridge, MA), anti‐XRCC6 antibody (Santa Cruz Biotech., Santa Cruz, CA), anti‐Tubulin monoclonal antibody (Sigma‐Aldrich, St. Louis, MO), anti‐Phospho‐Histone H2A.X monoclonal antibody (Cell Signaling), anti‐Histone H3 monoclonal antibody (Cell Signaling), and normal rabbit IgG (Santa Cruz Biotech.).
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