The Jurkat (ATCC, TIB-152), KOPN-8 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ], ACC 552), and NALM-16 (DSMZ, ACC 680) cell lines were kindly provided by Dr. Miles A. Pufall and Dr. Carlos Chan from the University of Iowa (Iowa City, IA, USA). Jurkat, KOPN-8, and NALM-16 cells were maintained in RPMI-160 medium (Gibco, 11875119) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals, S11550). Ea.Hy926 cells (ATCC, CRL-2922) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, 11965092) supplemented with 10% FBS. CHO WT and CHO 22+ cells were generously provided by Dr. James C. Paulson (The Scripps Research Institute (La Jolla, CA, USA). CHO WT and CHO 22+ cells were prepared as described previously.71 (link),72 (link) CHO WT and CHO 22+ cells were maintained in DMEM/F12 (Gibco, 11320033) supplemented with 10% FBS and DMEM/F12 supplemented with 10% FBS and 500 μg/mL Hygromycin-B (Roche, 10843555001), respectively. Cell lines were incubated at 37°C under 5% CO2 with medium changes every 2–3 days until confluent. Non-adherent cells were trypsinized using trypsin EDTA (0.25%; Gibco, 25200072). Cell lines were screened for mycoplasma contamination93 (link) and used within eight passages.
Rpmi 160 medium
Manufactured by Thermo Fisher Scientific
Sourced in China
RPMI 1640 medium is a commonly used cell culture medium formulated to support the growth and maintenance of a wide variety of cells. It provides essential nutrients, vitamins, and salts required for cell proliferation and survival. The medium is often used for culturing lymphocytes, hybridomas, and other suspension cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Ea hy926 cells, by American Type Culture Collection (1 mentions)
Dmem f12, by Roche (1 mentions)
Hygromycin b, by Roche (1 mentions)
Matrigel, by BD (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Stempro nsc sfm, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Polyethylenimine (pei), by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Co2 incubator, by Sanyo (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
5 protocols using rpmi 160 medium
1
Maintenance and Characterization of Cell Lines
2
Patient-Derived Glioblastoma Cell Lines
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Patient tumour tissue was collected following informed consent and with human ethics approval from the QIMR Berghofer Medical Research Institute and Royal Brisbane and Women's Hospital human research ethics committees. All human studies have been performed in accordance with the ethical standards laid down in the 2013 version of the 1964 Declaration of Helsinki. Tumour tissue was examined by a neuropathologist to determine tumour type and grade. Patient-derived cell lines [30 (link)–32 (link), 73 (link)–76 (link)] were established as reported previously [29 (link)]. These were cultured as adherent monolayers in matrigel (BD Biosciences)-coated vessels using RHB-A stem cell culture medium (StemCells Inc) supplemented with 20 ng/ml EGF (Gibco) and 10 ng/ml FGFb (Gibco) or as tumourspheres using StemPro NSC SFM (Invitrogen). U87 and U251 cells (obtained from The University of Queensland and authenticated by PCR-based short tandem repeat profiling by the QIMR-B DNA Sequencing and Genotyping facility within the past 12 months) were maintained in RPMI160 medium (Gibco) supplemented with 10% foetal bovine serum (Gibco), 2 mM L-glutamine, 25 mM HEPES, 25 mM sodium bicarbonate, 100 U/ml penicillin and 100 μg/ml streptomycin. Cells were cultured in 5% CO2 / 95% humidified air at 37°C. GBM subtyping was performed as described [25 (link)].
3
Cell Culture and Transfection Protocols
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HEK293T, HeLa, and TZM-bl cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 50 U/ml penicillin, and 50 μg/ml streptomycin (Invitrogen). SupT1 cells were grown in RPMI 160 medium (Invitrogen) supplemented with 10% FBS, 2 mM L-glutamine, 50 U/ml penicillin, and 50 μg/ml streptomycin. Transfection of plasmid DNAs or siRNAs was conducted with polyethyleneimine (PEI) (Sigma-Aldrich) or Lipofectamine 3000 (Invitrogen) according with the manufacturer’s instructions.
4
Culturing Human Lung Cell Lines
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The A549 and H1975 human LUAD cell lines and BEAS‐2B human normal lung fibroblast cell lines used in this study were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Science (Shanghai Institute of Cell Biology, China) and authenticated by STR typing. The A549 were cultured in Ham's F‐12K medium with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, Waltham, MA). H1975 were cultured in RPMI 1640 medium (Gibco, Life Technologies, California, USA) with 10% fetal bovine serum. The BEAS‐2B were cultured in were cultured in RPMI 160 medium (Invitrogen, Shanghai, China) supplemented with 10% FBS. The cells were placed in a CO2 incubator (SANYO Electric Co., Ltd., Japan) with constant 90% humidity and 5% CO2.
5
Culturing Pulmonary Adenocarcinoma and Bronchial Epithelial Cells
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The human pulmonary adenocarcinoma brain metastasis cell line PC-14/B was obtained from Shanghai Maisha Biotechnology Co., Ltd (Shanghai, China). The human bronchial epithelial cell line BEAS-2B was a gift from the Department of Physiology, the Second Military Medical University, Shanghai, China. The 293 T-cell line was purchased from the cell bank of the Chinese Academy of Sciences (Beijing, China). PC-14/B and BEAS-2B cells were cultured in RPMI160 medium (Invitrogen, Shanghai, China) supplemented with 10% fetal bovine serum (FBS), and 293 T-cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Invitrogen) plus 10% FBS. All cells were maintained at 37°C in 5% CO2.
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