The PET scans were conducted using two Siemens Inveon PET-CT scanners (Siemens Preclinical Solution, USA). Before each scan, the animals were weighed and anaesthetized with inhalation of 5% isoflurane in oxygen. A catheter was used to intravenously administer the tracer into the tail vein. 1.5% isoflurane was used for anaesthesia maintenance. During the scan, parameters such as respiration and temperature were continuously monitored using a Monitoring Acquisition Module (Minerve, France). An intravenous bolus injection of 5.4 ± 1.3 MBq (range 3.0-8.9 MBq) [ 11 C]UCB-J with a mass dose of 0.034 ± 0.007 μg (range 0.018-0.049 μg) was administered over 12 s using an automated pump (Pump 11 Elite, Harvard Apparatus, USA) at the start of the scanning procedure. Following the PET scan, a CT scan (10 min 80 kV/500 𝜇A) was performed for attenuation correction.
Inveon pet ct scanner
Manufactured by Siemens
Sourced in United States, Germany, United Kingdom
The Inveon PET/CT scanner is a laboratory equipment product developed by Siemens. It combines positron emission tomography (PET) and computed tomography (CT) imaging modalities in a single system. The Inveon PET/CT scanner allows for the acquisition of both functional and anatomical data from small animal subjects.
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Lab products found in correlation
Isoflurane, by Baxter (7 mentions)
Inveon research workstation software, by Siemens (4 mentions)
Inveon research workspace, by Siemens (3 mentions)
Flowcount b fc 3100 gamma detector, by Bioscan (3 mentions)
Vivoquant, by Invicro (3 mentions)
Pump 11 elite, by Harvard Apparatus (2 mentions)
Inveon research workplace software, by Siemens (2 mentions)
Pettrace cyclotron, by GE Healthcare (2 mentions)
Inveon research workplace workstation software, by Siemens (2 mentions)
Gamma counter, by PerkinElmer (2 mentions)
Irw software, by Siemens (1 mentions)
Micropet focus 220, by Siemens (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Fusion software, by Mediso (1 mentions)
Inveon research software, by Siemens (1 mentions)
Chelex, by Bio-Rad (1 mentions)
Ge pettrace cyclotron, by GE Healthcare (1 mentions)
Axima performance maldi tof ms, by Shimadzu (1 mentions)
Avance 500 mhz spectrometer, by Bruker (1 mentions)
Richard allan scientific decalcifying solution, by Thermo Fisher Scientific (1 mentions)
74 protocols using inveon pet ct scanner
1
PET Imaging of [11C]UCB-J in Mice
2
In Vivo Imaging of Synaptic Density
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Small-animal PET/CT imaging was performed on 2 Siemens Inveon PET/CT scanners (Siemens Preclinical Solutions). Animal preparation was performed as previously described (20 (link)). At the start of the dynamic small-animal PET scan, animals were injected via the tail vein with a bolus of 11C-UCB-J (5.4 ± 1.3 MBq) over a 12-s interval (1 mL/min) by use of an automated pump (Pump 11 Elite; Harvard Apparatus). The activity was injected in a trace dose, keeping the cold mass within 2.0 μg/kg across time points for consistency. Data were acquired in list-mode format. After the small-animal PET scan, a 10-min CT scan (80 kV; 500 μA) was performed for coregistration and attenuation correction. Detailed information on the scan parameters is reported in Supplemental Table 1 (supplemental materials are available at http://jnm.snmjournals.org ). Published work from our group (20 (link)) was reanalyzed for blocking validation of 11C-UCB-J binding in the spinal cord. Blocking was achieved by pretreatment with levetiracetam injected intraperitoneally at either 50 (n = 4) or 200 (n = 4) mg/kg 30 min before radioligand delivery. Representative SUV images were generated on the basis of the interval from 10 to 90 min.
3
Imaging Inflammatory Biomarkers with PET
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PET imaging was conducted using Siemens Inveon PET/CT scanners (Siemens Preclinical Solutions, Knoxville, TN, USA). Animals were administered approximately 100 μCi (71–105) 68Ga-VCAM-MPIO or 68Ga-IgG-MPIO via indwelling tail vein catheter and scanned for 45 minutes. All PET scans were immediately followed by a CT scan for anatomical reference and attenuation correction of PET data. Data were iteratively reconstructed to a voxel size of 0.4×0.4×0.8 mm3. Ellipsoidal regions of interest (ROIs) were manually defined around the heart, liver, spleen, and tumor. Decay-corrected signal intensity was measured as percentage of the injected dose per gram tissue (%ID/g).
4
Dynamic microPET/CT Imaging Protocol
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Two Siemens Inveon PET/CT scanners (Siemens Preclinical Solution, Knoxville, USA) were used to acquire the dynamic microPET/computed tomography (CT) images. Animal preparation was performed as previously described [11 (link), 12 (link)]. Details of the PET acquisition are provided in the ESM.
5
PET Imaging of Tumor Tracer Uptake in Xenograft Models
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89Zr-PET imaging studies of tumor tracer uptake were performed after the intravenous injection of a single dose of 5 mg/kg of the mAbs targeting TENB2, STEAP1, or gD (isotype control) radiolabeled with 89Zr (3.7 MBq; 30 MBq/mg), in LuCaP35V, LuCaP70, LuCaP77, and LuCaP96.1 patient-derived xenografts (n = 3-5 per group). PET imaging was conducted five days later using Siemens Inveon PET/CT scanners (Siemens). Animals were lightly anesthetized for restraint with approximately 3.5% sevoflurane, and body temperature was maintained at 37°C by warm airflow. PET scans were 15-30 minute static scans.
Region of interest measurements defined by using software tools were made on multiple axial slices of the tissues using IRW software (Siemens). Decay-corrected signal intensity of tumor was measured as percentage of the injected dose per gram (%ID/g), assuming a tissue density of 1 ml per 1 gram soft tissue.
Region of interest measurements defined by using software tools were made on multiple axial slices of the tissues using IRW software (Siemens). Decay-corrected signal intensity of tumor was measured as percentage of the injected dose per gram (%ID/g), assuming a tissue density of 1 ml per 1 gram soft tissue.
6
In Vivo PET Imaging of B16F10 Xenografts
7
Micro-CT analysis of subchondral bone
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In vitro high-resolution micro-computed tomography (micro-CT) images were obtained using an Inveon PET/CT scanner (Siemens). We dissected the femur from control or LLC-bearing mice and fixed them in 4% PFA for 48 h. The Inveon Research Workplace 4.1 software was used to reconstruct and analyze the images. The whole subchondral bone medial compartment was defined as the reconstruction area, and three-dimensional structure analysis was performed. The three-dimensional structural parameters analyzed included trabecular bone volume per tissue volume (BV/TV), bone surface area/TV (bone surface area per tissue volume), trabecular number (Tb.Nu), trabecular pattern factor (Tb.Pf), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), and cortical wall thickness.
8
In Vivo Imaging of Bladder Cancer Uptake
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At 15 d after the inoculation of UMUC3 BCa cells, mice (3 per group) were either intravenously administered 18F-labeled galactodendritic unit 4 (2.9–3.3 MBq) or intravesically administered (i.e., instillation directly into the bladder via insertion of an urethral catheter) 14.7–15.3 MBq of 18F-galactodendritic unit 4 or 18F-FDG.
At 0.5, 1, and 2 h after intravenous administration of 18F-labeled galactodendritic unit4, PET images were recorded on an Inveon PET/CT scanner (Siemens).
At 30 min after intravesical administration, the mice were anesthetized with 1.5%–2% isoflurane, the bladder was fully emptied and flushed with PBS, and PET images were recorded (following previously reported methodology (21 (link))) at 1 h after intravesical administration. All images were visualized in AMIDE software (version 1.0.4;http://amide.sourceforge.net ).
Acute biodistribution studies were performed at 2 h after intravenous injection of 18F-galactodendritic unit4 (21 (link)), and the radioactivity associated with each organ was expressed as a percentage of injected dose per gram of organ.
At 0.5, 1, and 2 h after intravenous administration of 18F-labeled galactodendritic unit
At 30 min after intravesical administration, the mice were anesthetized with 1.5%–2% isoflurane, the bladder was fully emptied and flushed with PBS, and PET images were recorded (following previously reported methodology (21 (link))) at 1 h after intravesical administration. All images were visualized in AMIDE software (version 1.0.4;
Acute biodistribution studies were performed at 2 h after intravenous injection of 18F-galactodendritic unit
9
PET/CT Imaging of Phantoms and Rats with Rubidium-82
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The phantoms and rats underwent PET/CT scan using the Siemens Inveon PET/CT scanner. The phantom was injected with ~ 50 MBq 82Rb and underwent a ten-minute PET acquisition. After the PET acquisition a CT scan was performed for segmentation and attenuation. The rats were anesthetized prior to PET/CT scan with Sevoflurane 3–4% and a 24G intravenous catheter (Vasofix Safety, Braun, Denmark) was placed in the tail vein. The rats were first CT scanned for attenuation correction. Then ~ 60 MBq 82Rb was administered and a dynamic 10-min PET scan was performed. The rats were monitored with ECG and a respiratory sensor during the PET/CT.
10
Zirconium-89 Imaging of GPC3 in Mice
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89Zr-DFO-αGPC3 imaging studies were performed using the Inveon PET/CT scanner (Siemens Medical Solutions USA, INC. Molecular Imaging, Knoxville, TN), which was calibrated for 89Zr. Whole-body PET and CT imaging was performed on animals anesthetized with 1–2% isoflurane anesthesia in 100% oxygen at 1L/min in a temperature-controlled bed with respiratory monitoring. Tumor-bearing animals were injected with 11.1 MBq (300uCi) of 89Zr-DFO-αGPC3 (~ 70 μg antibody) via the tail vein 1 week before (n = 29) and 4 weeks (n = 27) after RIT injection. Pre-RIT PET/CT imaging was performed on days 4 and 5 after 89Zr-DFO-αGPC3 injection and post-RIT imaging was performed on days 6 and 7 after injection due to equipment availability. Animals first had a 60-min PET scan followed by a CT scan, which enabled scatter and attenuation correction. Due to scanner malfunctions during post-RIT imaging, 10 animal scans were PET-only and therefore not corrected for scatter or attenuation. Due to limited imaging time on PET scanner, not all animals were imaged. Animals were randomly selected and were imaged before and after RIT.
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