Fuji dri chem

Manufactured by Fujifilm

Sourced in Japan

The Fuji DRI-CHEM is a compact, automated chemistry analyzer designed for clinical laboratory testing. It utilizes dry-slide technology to provide fast and reliable results for a range of common clinical chemistry parameters.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Labassay creatinine kit, by Fujifilm (1 mentions) Blood glucose meter, by Abbott (1 mentions) Enzyme linked immunosorbent assay, by Fortis Life Sciences (1 mentions) Bm 6050, by JEOL (1 mentions) Enzyme immunoassay, by Takara Bio (1 mentions)

13 protocols using fuji dri chem

Canine Insulin and Glucose Measurement

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In both experiments, corrected blood samples were placed in chilled polyethylene terephthalate tubes containing EDTA-2Na and aprotinin (NT-EA0205, Nipro, Tokyo, Japan) to prevent the
deterioration of insulin activity. Plasma was separated by centrifugation (4°C, 1,000 g, 15 min) and stored at −80°C until assayed. Plasma insulin concentrations were determined using a
validated sandwich ELISA kit (Mercodia Canine Insulin ELISA; Mercodia, Inc., Uppsala, Sweden), which can determine canine insulin concentrations from 0.02 to 1.5
ng/ml. Plasma glucose concentrations were determined with dry-slide technology using a multilayered analytical film (Fuji DRI-CHEM, Fujifilm, Tokyo,
Japan).

TAKASHIMA S., TAKITANI S.I., KITAMURA M., NISHII N., KITAGAWA H, & SHIBATA S. (2019). Effect of cyclooxygenase-2 inhibitors at therapeutic doses on body temperature during anesthesia in healthy dogs administered with amino acids. The Journal of Veterinary Medical Science, 81(9), 1379-1384.

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Biomarker Measurements for Metabolic Health

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Serum triglyceride and total cholesterol levels were measured (FUJI DRI-CHEM; Fujifilm, Tokyo, Japan). Urinary glucose levels were measured using a blood glucose meter (Abbott, Japan). Urinary creatinine levels were measured using a LabAssay Creatinine Kit (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan). Urinary protein levels were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). The insulin in the serum and the liver-type fatty acid-binding protein (L-FABP) in the urine and serum were measured in duplicate using a commercially available enzyme-linked immunoassay kit (#10-1247-01; Mercodia AB, Uppsala, Sweden, and RFBP10; R&D system, Minneapolis, MN, USA).

Saitoh S., Takaki T., Nakajima K., Wo B., Terashima H., Shimo S., Nguyen H.B., Thai T.Q., Kumamoto K., Kunisawa K., Nagao S., Tojo A., Ohno N, & Takahashi K. (2023). Treatment of tubular damage in high-fat-diet-fed obese mice using sodium-glucose co-transporter inhibitors. PLOS ONE, 18(2), e0281770.

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Quantifying Human Albumin in Rat Blood

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Whole blood samples were centrifuged (4000 revolutions per min) for 20 min at 4 °C, and the supernatants were collected and stored at −30 °C. Subsequently, the supernatants were tested using a serum multiple biochemical analyzer (Fuji Drichem; Fuji Film Inc., Tokyo, Japan). The human albumin level in the rat blood was assessed by enzyme linked immunosorbent assay (Bethyl Laboratories, Montgomery, TX, United States), according to the manufacturer’s instructions.

Tsuchida T., Murata S., Matsuki K., Mori A., Matsuo M., Mikami S., Okamoto S., Ueno Y., Tadokoro T., Zheng Y.W, & Taniguchi H. (2019). The Regenerative Effect of Portal Vein Injection of Liver Organoids by Retrorsine/Partial Hepatectomy in Rats. International Journal of Molecular Sciences, 21(1), 178.

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Biochemical Evaluation of CKD Mice

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BUN and serum creatinine were measured using FUJI DRI-CHEM (Fujifilm, Tokyo, Japan). The values for the biochemical evaluation in plasma from CKD mice are listed in Table 1.

Tanaka S., Watanabe H., Nakano T., Imafuku T., Kato H., Tokumaru K., Arimura N., Enoki Y., Maeda H., Tanaka M., Matsushita K., Fukagawa M, & Maruyama T. (2020). Indoxyl Sulfate Contributes to Adipose Tissue Inflammation through the Activation of NADPH Oxidase. Toxins, 12(8), 502.

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Postprandial Blood Glucose Measurement

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Capillary blood glucose levels were measured once at each visit using the glucose–oxidase method (Fuji DRI‐CHEM; Fuji Film, Tokyo, Japan), regardless of fasting or postprandial status, and are expressed as plasma equivalents. At each visit, a clinical laboratory technician checked the time when the patients began to eat their final meal, and recorded the postprandial time interval in 15‐min units. Blood glucose levels measured at 1–2 h after breakfast and after lunch, those after breakfast, and those after lunch were defined as 1–2 h post‐breakfast and post‐lunch blood glucose (1–2h‐PBLBG) levels, 1–2 h post‐breakfast blood glucose (1–2h‐PBBG) levels, and 1–2 h post‐lunch blood glucose (1–2h‐PLBG) levels, respectively. Intrapersonal mean 1–2h‐PBLBG, 1–2h‐PBBG and 1–2h‐PLBG levels during 2 years from the first visit were used as baseline data.

Takao T., Takahashi K., Yoshida Y., Kushiyama A., Onishi Y., Tahara T., Shimmei A., Kikuchi T., Suka M., Yanagisawa H., Iwamoto Y, & Kasuga M. (2020). Effect of postprandial hyperglycemia at clinic visits on the incidence of retinopathy in patients with type 2 diabetes: An analysis using real‐world long‐term follow‐up data. Journal of Diabetes Investigation, 11(4), 930-937.

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Evaluating Liver Injury Markers

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Normal ranges for human of ALT and LDH were 6-30 U/l and 119-229 U/l, respectively. In this study, we calculated ALT/LDH ratio with the following formula: ALT/LDH ratio=(serum ALT-ULN)/(serum LDH-ULN), where ULN stands for the upper limit of normal.
Blood samples of mice were withdrawn from the tail vein 6 h after the injection of ConA or TNFα. Serum levels of ALT, LDH, and fibrin degradation products (FDP) for mice were measured using chemical analyzer Fuji DRI-CHEM (Fuji Film) and FDP-ELISA kit (MyBioSource), respectively.

Kuwano A., Kurokawa M., Kohjima M., Imoto K., Tashiro S., Suzuki H., Tanaka M., Okada S., Kato M, & Ogawa Y. (2021). Microcirculatory disturbance in acute liver injury. Experimental and Therapeutic Medicine, 21(6), 596.

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Serum Phosphate Homeostasis Analysis

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Serum samples were used to quantify selected parameters of P homeostasis. Levels of inorganic P, Ca, magnesium (Mg) and the activity of alkaline phosphatase (ALP) were analyzed with commercial assays using Fuji DriChem (FujiFilm, Minato, Japan).

Oster M., Reyer H., Gerlinger C., Trakooljul N., Siengdee P., Keiler J., Ponsuksili S., Wolf P, & Wimmers K. (2021). mRNA Profiles of Porcine Parathyroid Glands Following Variable Phosphorus Supplies throughout Fetal and Postnatal Life. Biomedicines, 9(5), 454.

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Metabolic Assessment of Mice under Pharmacological Intervention

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Whole-body energy expenditure of mice was assessed with a metabolic chamber (ARCO2000-RAT/ANI System; Arco) as described previously (23 (link)). Mice were injected intraperitoneally with vehicle (saline) or CL316243 at a dose of 0.01 mg/kg during the measurements. The respiratory quotient (VCO₂/VO₂) was calculated based on Weir (25 (link)). Spontaneous activity of the mice was simultaneously measured with an activity sensor (NS-AS01; Neuroscience). All measurements were performed at 4.5-min intervals.
For the glucose tolerance test, mice were injected with glucose 1 g/kg i.p. after a 6-h fast. An insulin tolerance test was performed by injecting insulin 0.75 units/kg after an overnight fast. Blood glucose levels were measured with blood glucose test strips (Arkary) postinjection at 15, 30, 60, and 120 min. Fasting plasma glucose levels were determined with FUJI DRI-CHEM (Fujifilm). Plasma insulin levels were measured with a commercially available ELISA (Morinaga). Plasma triglyceride (TG) levels were measured with a Wako TG test kit. Total liver lipids were extracted with a chloroform:methanol mixture (2:1 volume for volume) as described by Folch et al. (26 (link)). The liver TG concentration in the lipid extracts was also measured with the Wako TG test.

Ohyama K., Nogusa Y., Shinoda K., Suzuki K., Bannai M, & Kajimura S. (2016). A Synergistic Antiobesity Effect by a Combination of Capsinoids and Cold Temperature Through Promoting Beige Adipocyte Biogenesis. Diabetes, 65(5), 1410-1423.

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Serum Biochemical Analysis Protocol

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Whole-blood samples were centrifuged for 10 min at 4 °C and 1200×g. Supernatants were collected and stored at −80 °C until further testing using a serum multiple biochemical analyzer (Fuji Drichem; Fujifilm Inc., Tokyo, Japan) to measure the aspartate transaminase, alanine aminotransaminase, ammonia, gamma-glutamyl transpeptidase (gGTP), lactate dehydrogenase (LDH), total and direct bilirubin, and albumin levels.

Tsuchida T., Murata S., Hasegawa S., Mikami S., Enosawa S., Hsu H.C., Fukuda A., Okamoto S., Mori A., Matsuo M., Kawakatsu Y., Matsunari H., Nakano K., Nagashima H, & Taniguchi H. (2020). Investigation of Clinical Safety of Human iPS Cell-Derived Liver Organoid Transplantation to Infantile Patients in Porcine Model. Cell Transplantation, 29, 0963689720964384.

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Biomarker Measurement in Blood Samples

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Blood samples were collected every 2 weeks, and then plasma or serum was extracted. ALT activity was measured by Fuji DRI-CHEM (Fuji FILM, Tokyo, Japan). Blood h-Alb levels were measured by immunonephelometry in a JEOL BM6050 autoanalyzer (JEOL, Tokyo, Japan) using LZ Reagent Eiken Alb II (Eiken Chemical, Tokyo, Japan). The plasma or serum levels of h-ALT1 and mouse M2BP level were measured according to the manufacturer’s protocols by using h-ALT1 ELISA kit (PhoenixBio, Co., Ltd., Higashihiroshima, Japan) [34 (link)] and Mac-2 Binding Protein assay kit (IBL Co., LTD., Fujioka, Japan), respectively.

Kisoh K., Sugahara G., Ogawa Y., Furukawa S., Ishida Y., Okanoue T., Kohara M, & Tateno C. (2021). Estimating Drug Efficacy with a Diet-Induced NASH Model in Chimeric Mice with Humanized Livers. Biomedicines, 9(11), 1647.

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