Phosphate buffer saline (PBS), doxorubicin (DOXO), deuterium oxide (D2O), DNA sodium salt from calf thymus, sodium azide (NaN3), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) and the photosensitizing agents pheophorbide a (PhA) and Rose Bengal (RB) were purchased from Sigma-Aldrich, Chemical Co. (St. Louis, MO, USA). LysoTracker Green DND-26 was purchased from Thermo Fisher (Waltham, MA, USA). Flavin mononucleotide (FMN) was from Chemodex Ltd. (St. Gallen, Switzerland).
3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt
Manufactured by Merck Group
Sourced in United States, China, Germany
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a yellow tetrazolium salt. It is used as a reagent in cell-based assays to assess cell metabolic activity.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions)
Doxorubicin, by Merck Group (1 mentions)
Pheophorbide a pha, by Merck Group (1 mentions)
Lysotracker green dnd 26, by Thermo Fisher Scientific (1 mentions)
Phosphate buffer saline (pbs), by Merck Group (1 mentions)
Deuterium oxide, by Merck Group (1 mentions)
Eugenol, by Merck Group (1 mentions)
Imatinib mesylate, by Merck Group (1 mentions)
Ficoll hypaque, by Merck Group (1 mentions)
Baicalein, by Meilun (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Matrigel, by BD (1 mentions)
Cycloheximide (chx), by MedChemExpress (1 mentions)
34 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt
1
Phototoxic Effect of Doxorubicin and Photosensitizers
2
Antifungal MIC Determination of Eugenol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The minimum inhibitory concentration (MIC) for eugenol (Sigma–Aldrich, St. Louis, MO, United States) was determined by the antifungal microdilution susceptibility standard test proposed by the CLSI M27-A3 method (Institute Clinical and Laboratory Standards, 2008 ). The inoculum was prepared in sterile saline and the transmittance of the suspensions was adjusted to 75–77% (530 nm), followed by further dilution in RPMI-1640 buffered with MOPS (Sigma–Aldrich, St Louis, MO, United States) medium to achieve 1.0 × 103 to 5.0 × 103 CFU/mL. The final concentrations ranged from 2 to 1024 mg/L for eugenol. The plates were incubated at 35°C for 72 h. The MIC for eugenol was determined visually as 100% growth inhibition when compared to the control. The results were confirmed by adding the salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) (Sigma–Aldrich, St Louis, MO, United States) (5.0 mg/mL) to determine the reduction in the metabolic cell activity. Briefly, the plates were incubated at 35°C for 3 h and DMSO was added before spectrophotometric reading at 490 nm. The MIC endpoint for interpreting the results was 100% of reduction in metabolic activity for eugenol compared with the control. The isolate Candida parapsilosis ATCC 22019 was used as a quality control. All the tests were performed in duplicate for each strain.
3
Synthesis and Characterization of Tyrosol Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two series of tyrosol derivatives bearing 3,5-disubstituted isoxazole (3a –e ) and 1,4-disubstituted triazole (4a –e ) were synthesized and characterized as described previously by Aissa et al. [21 (link)]. Imatinib mesylate (STI571), used as a reference therapeutic molecule, Ficoll–Hypaque and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Botanical Compounds Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Baicalin and baicalein (purity > 95%, HPLC) were purchased from Meilun Bio (Dalian, China). Wogonin (purity > 99%, HPLC) was purchased from Desite Bio (Chengdu, China). Baicalin, baicalein and wogonin were dissolved in DMSO at 200 mM, 10 mM or 40 mM respectively and stored at -20°C. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was from Sigma-Aldrich (Shanghai, China). Matrigel was purchased from BD Biocoat (New Jersey, USA). Cycloheximide (CHX) was from MedChem Express (New Jersey, USA). LY294002 was from Cell Signaling Technology. α-bungarotoxin(α-BT) was obtained from Abcam Company. PP2 was purchased from Enzo Biochem.
5
In Vitro Antioxidant and Hepatoprotective Activities
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SLB, SLM, 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and CCl4 (99.9%) were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). SLP was purchased from Medix, S.A. de C.V. (México City, D.F. México), and dimethyl sulfoxide (DMSO) was purchased from ACS Research Organics (Cleveland, OH, USA). Total antioxidant capacity (TAOxC), GSH, superoxide dismutase (SOD), and thiobarbituric acid reactive substances were purchased from Kit OXItek (Buffalo, NY, USA). Dulbecco’s modified Eagle’s medium advanced (DMEMA) with and without phenol red, fetal bovine serum, trypsin 0.25% (1×), penicillin G (100 IU/mL), streptomycin (100 μg/mL), and phosphate-buffered saline (PBS) were purchased from Gibco Invitrogen (Carlsbad, CA, United States). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) activities were measured using an ILab 300 Plus chemistry analyzer (Instrumentation Laboratory, Bedford, MA, USA).
6
Endothelin-1 Receptor Antagonist Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Endothelin-1 was obtained from Tocris Biosciences (Bristol, UK). Ambrisentan, bosentan, and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Dulbecco’s Modified Eagle Medium (DMEM), phosphate-buffered saline (PBS), 0.25% trypsin-EDTA solution, fetal bovine serum (FBS), penicillin/streptomycin (P/S), and other cell culture reagents were acquired from Gibco (Grand Island, NY, USA).
7
Cultivation of HEK293, RAW264.7, and Trypanosoma cells
8
Chlorpyrifos Cytotoxicity Assay Protocol
9
Evaluating L-citrulline's Effects on HeLa Cell Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human cervix adenocarcinoma HeLa (ATCC® CCL-2™) cells were obtained from the American Type Culture Collection (Manassas, USA). L-citrulline was obtained from Akcan Kimya (Turkey); fetal bovine serum (FBS), penicillin/streptomycin, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-2H-tetrazolium bromide (MTT) and Eagle's minimum essential medium (EMEM) were purchased from Sigma-Aldrich (St. Louis, USA). Caspase 3/7 and Annexin-V Kits were from Merck, Millipore, USA. All analyses were repeated three times, and the average of the obtained values was accepted as the analysis result.
10
Synthesis and Characterization of Cholesterol-Conjugated HSP27 Inhibitor
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemical spectral analyses were conducted at Kuwait University Research Center. Thin layer chromatography (TLC) was performed using Polygram SIL G/UV 254 TLC plates, and the results were visualized under ultraviolet light at 254 and 350 nm, with hexane/ethyl acetate as a solvent. Column chromatography was performed using silica gel 60A with a mesh size of 40–60 µm. 1H and 13C nuclear magnetic resonance (NMR) spectra were obtained using a Bruker DPX 600 NMR spectrophotometer at 600 MHz and 150 MHz in DMSO and CDCL3, respectively. Mass spectra were detected on a GC-MS DFS–Thermo spectrometer. An IR spectrum was obtained with a Jasco 6300 FTIR spectrometer. Melting point was determined using a Netzsch DSC 204 F1 Phoenix differential scanning calorimeter.
SKOV3 human ovarian cancer cells were obtained from American Type Culture Collection. A cholesterol-conjugated HSP27 inhibitor was synthesized in our laboratory. Cell culture media and other supplements, such as 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), were obtained from Sigma-Aldrich (Milwaukee, WI). Chemicals and reagents are commercially available and ready for direct use, requiring no preparation. Biological parameters were examined at the Biology Department, Kuwait University.
SKOV3 human ovarian cancer cells were obtained from American Type Culture Collection. A cholesterol-conjugated HSP27 inhibitor was synthesized in our laboratory. Cell culture media and other supplements, such as 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), were obtained from Sigma-Aldrich (Milwaukee, WI). Chemicals and reagents are commercially available and ready for direct use, requiring no preparation. Biological parameters were examined at the Biology Department, Kuwait University.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!