Fluorescence lifetime of both free HSA and HSA-EA were measured using TCSPC instrument (Fluoro Hub; Horiba JobinYvon, GB). The samples were excited at 295 nm using a Nano-LED pulsed laser. The emission decay data at 330 nm were analysed using the DAS6.4 software provided with the instrument [17] (link).
Fluorohub
Manufactured by Horiba
Sourced in United Kingdom
The FluoroHub is a modular and scalable fluorescence spectroscopy platform designed for versatile laboratory applications. It provides a comprehensive solution for diverse fluorescence-based measurements and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Cary 5000, by Agilent Technologies (1 mentions)
Tbx ps photon detection module, by Horiba (1 mentions)
Quanta phi, by Horiba (1 mentions)
Cary 5000 spectrophotometer, by Agilent Technologies (1 mentions)
Fluorolog 3 spectrofluorometer, by Horiba (1 mentions)
Quantaurus qy absolute pl quantum yield spectrometer c11347 11, by Hamamatsu Photonics (1 mentions)
Fp 6300 spectrofluorometer, by Jasco (1 mentions)
Nanoled, by Horiba (1 mentions)
Cary 5000 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
5 protocols using fluorohub
1
Fluorescence Lifetime Measurement of HSA
2
Optical Characterization of Carbon Dots
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A Cary
5000 (Varian) UV/vis/NIR spectrophotometer and a Fluorolog 3 spectrofluorimeter
(HORIBA Jobin-Yvon) were used to record the UV–vis absorption
or PL emission spectra, respectively. PL emission absolute QY of C-Dots
and of the luminescent nanoformulations dispersed in solution was
evaluated using a “Quanta-phi” (HORIBA Jobin-Yvon) integrating
sphere coated by Spectralons. Time-correlated single photon counting
(TCSPC) measurements were performed with a FluoroHub (HORIBA Jobin-Yvon)
to investigate fluorescence lifetime of C-Dots, bare in CHCl3 dispersion and after their encapsulation in the PLGA nanoformulations.
A picosecond laser diode (NanoLED 375L) emitting τ ≈
80 ps pulses at a 1 MHz repetition rate was used as the excitation
source. The PL signals were detected by a picosecond photon counter
(TBX ps Photon Detection Module, HORIBA Jobin-Yvon) with a temporal
resolution of ∼200 ps.
5000 (Varian) UV/vis/NIR spectrophotometer and a Fluorolog 3 spectrofluorimeter
(HORIBA Jobin-Yvon) were used to record the UV–vis absorption
or PL emission spectra, respectively. PL emission absolute QY of C-Dots
and of the luminescent nanoformulations dispersed in solution was
evaluated using a “Quanta-phi” (HORIBA Jobin-Yvon) integrating
sphere coated by Spectralons. Time-correlated single photon counting
(TCSPC) measurements were performed with a FluoroHub (HORIBA Jobin-Yvon)
to investigate fluorescence lifetime of C-Dots, bare in CHCl3 dispersion and after their encapsulation in the PLGA nanoformulations.
A picosecond laser diode (NanoLED 375L) emitting τ ≈
80 ps pulses at a 1 MHz repetition rate was used as the excitation
source. The PL signals were detected by a picosecond photon counter
(TBX ps Photon Detection Module, HORIBA Jobin-Yvon) with a temporal
resolution of ∼200 ps.
3
Characterization of Synthesized Carbon Dots
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The chemical composition of synthesized CDs was investigated with Fourier-Transform Infrared (FT-IR) spectroscopy, using a PerkinElmer Spectrum One Fourier Transform Infrared spectrophotometer (PerkinElmer, Inc., Waltham, MA, USA) with the attenuated total reflection technique, using a 4-mm-diameter diamond microprism as an internal reflection element.
UV–Vis absorption spectra were recorded with a Cary 5000 spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA). Steady-state photoluminescence excitation (PLE) and emission (PL) spectra of CDs solution and nanocomposites were acquired using a Fluorolog 3 spectrofluorometer (HORIBA Jobin-Yvon GmbH, Bensheim, Germany), equipped with double-grating excitation and emission monochromators and a 450W Xe lamp as light source. Absolute PLQY was measured using a “Quanta-phi” integration sphere coated with Spectralon® (HORIBA Jobin Yvon GmbH, Bensheim, Germany) (reflectance ≥95% in the range 250–2500 nm). Time-Resolved PL (TRPL) measurements were carried out by the Time Correlated Single Photon Counting (TCSPC) technique, with a FluoroHub (HORIBA Jobin-Yvon). CDs solutions were excited using 80 ps laser diode sources at 375 nm (NanoLED 375L) and at 485 nm (NanoLED 485L), with a final resolution time of 300 ps.
UV–Vis absorption spectra were recorded with a Cary 5000 spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA). Steady-state photoluminescence excitation (PLE) and emission (PL) spectra of CDs solution and nanocomposites were acquired using a Fluorolog 3 spectrofluorometer (HORIBA Jobin-Yvon GmbH, Bensheim, Germany), equipped with double-grating excitation and emission monochromators and a 450W Xe lamp as light source. Absolute PLQY was measured using a “Quanta-phi” integration sphere coated with Spectralon® (HORIBA Jobin Yvon GmbH, Bensheim, Germany) (reflectance ≥95% in the range 250–2500 nm). Time-Resolved PL (TRPL) measurements were carried out by the Time Correlated Single Photon Counting (TCSPC) technique, with a FluoroHub (HORIBA Jobin-Yvon). CDs solutions were excited using 80 ps laser diode sources at 375 nm (NanoLED 375L) and at 485 nm (NanoLED 485L), with a final resolution time of 300 ps.
4
Fluorescence Characterization of Oligonucleotides
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Fluorescence measurements were obtained using 3 mm path length JASCO FMM-100 quartz microcells on a JASCO FP-6300 Spectrofluorometer equipped with a JASCO EHC-573 temperature controller. The emission spectra were recorded from 220 nm to 600 nm at a scan rate of 100 nm min−1 with an excitation wavelength of 374 nm for FPdC and 1C, and 365 nm for IM-CT. Quantum yields were measured with a Hamamatsu Photonics Quantaurus-QY C11347-11 Absolute PL Quantum Yield Spectrometer and calculated with the integrated measurement software. Fluorescence lifetime measurements were obtained using TCSPC (time-correlated single photon counting) on a Horiba Scientific Fluorohub equipped with a Horiba Scientific FluoroCube 3000U-SHK and a 375 nm Horiba Scientific NanoLED pulsed diode light source. Emission wavelength was set at the emission maximums found through fluorescence spectroscopy of each ODN. TAC range was 200 ns and Repetition rate was 1 MHz. The data was fitted to exponential functions using DAS6 (HORIBA).
5
Photophysical Properties of Pyrene Derivatives
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The toluene solutions of the trans- and tetrasubstituted pyrenes (ACS spectroscopic grade, Sigma-Aldrich) were subjected to sonication and heating cycles, and then left to cool to rt before recording the absorption and emission spectra. Absorption spectra were recorded using air-equilibrated solutions at rt, with an Agilent Cary 5000 UV–vis spectrophotometer using quartz cells with a path length of 1.0 cm. Emission spectra were recorded on an Agilent Cary Eclipse fluorescence spectrofluorometer. Emission lifetime measurements were performed on a JobinYvon-Horiba FluoroHub single-photon-counting module, using nano-LED-pulsed sources at 295 or 372 nm. The quantum yield measurements were performed using the relative determination, with coumarin 153 (C153, ϕ = 0.53 in ethanol) as the standard (st) [87 (link)]. The fluorescence quantum yields were then calculated according to Equation 1 :
Therein, I is the measured integrated fluorescence emission intensity, η is the refractive index of the solvent, and ϕ is the quantum yield.
Therein, I is the measured integrated fluorescence emission intensity, η is the refractive index of the solvent, and ϕ is the quantum yield.
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