Mir 301a 3p mimic

Following treatment of ox-LDL for 24 h, ox-LDL-induced HUVECs were inoculated into six-well plates until 80% confluence. HUVECs were respectively transfected with miRNA inhibitor-negative control (NC; 5 nM; cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd.), miR-301a-3p inhibitor (5 nM; cat. no. miR20000688-1-5; Guangzhou RiboBio Co., Ltd.), miRNA mimic-NC (5 nM; cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd.), miR-301a-3p mimic (5 nM; cat. no. miR10000688-1-5; Guangzhou RiboBio Co., Ltd.), small interfering RNA (si) R-NC (5 nM; cat. no. siN0000002-1-5; Guangzhou RiboBio Co., Ltd.) and siKLF7 (5 nM; cat. no. siG000008609A-1-5; Guangzhou RiboBio Co., Ltd.) using Lipofectamine^® 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h at 37˚C. Subsequent experiments were conducted 48 h after transfection.

miR-301a-3p mimic (5′-CAGUGCAAUAGUAUUGUCAAAGC-3′) and negative control (NC mimic, 5′-UCUACUCUUUCUAGGAGGUUGUGA-3′) were purchased from Guangzhou RiboBio Co., Ltd. miR-301a-3p inhibitor (5′-GCUUUGACAATCTATTGCACTG-3′) and negative control (NC-in, 5′-ACCGCUAAUCAUACGAAUACAC-3′) were purchased from Guangzhou RiboBio Co., Ltd. Plasmids (pcDNA3.1) expressing BTG1 and lentiviruses expressing miR-301a-3p were purchased from Hanbio Biotechnology Co., Ltd. Oligonucleotide (100 nM) transfection was performed using Lipofectamine^® 2000 (Thermo Fisher Scientific, Inc.). After 48 h of transfection, cells were collected for further investigation.