Histopathological sections were stained with anti Ki-67 (RM9106, 1:200, Thermo Scientific, Fremont, CA, USA); anti CD20 (1:400, Agilent Dako, Santa Clara, CA, USA), anti CD68 (1:150, Agilent Dako), anti CD30 (1:30, Agilent Dako), and anti TOX (HPA018322, 1:100, Sigma-Aldrich, Zwijndrecht, The Netherlands). Slides were evaluated by a highly experienced dermatopathologist (M.F.). The dermal infiltrate was evaluated semi-quantitatively and scored for percentage of immunopositive cells from a minimum of 3 representative medium-power fields, as follows: For CD30, CD20, CD68 and Ki-67: ≤ 5%, 6–10%, 11–20%, ≥ 21%. For TOX: ≤5%, 6–33%, 34–66% and ≥ 67%. The number of cases stained with each antibody is shown in Table I .
Anti cd30
Manufactured by Agilent Technologies
Sourced in United States
Anti CD30 is a laboratory reagent used for the detection and analysis of CD30 protein expression. CD30 is a cell surface receptor that is expressed on activated T and B cells, as well as in certain hematological malignancies. This reagent can be used in various laboratory techniques, such as flow cytometry, to identify and quantify cells expressing the CD30 marker.
Automatically generated - may contain errors
Lab products found in correlation
Anti ki67, by Thermo Fisher Scientific (1 mentions)
Anti cd20, by Agilent Technologies (1 mentions)
Anti tox hpa018322, by Merck Group (1 mentions)
Anti cd68, by Agilent Technologies (1 mentions)
Formalin, by Merck Group (1 mentions)
Hematoxylin eosin, by Bio-Optica (1 mentions)
Aperio scanscope, by Leica camera (1 mentions)
2 protocols using anti cd30
1
Immunohistochemical Profiling of Cutaneous T-cell Lymphoma
2
Immunohistochemical Analysis of Mouse Kidneys and Human Pancreas
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Explanted mouse kidneys and samples from human pancreas were fixed in 10% formalin (Sigma), paraffin-embedded and processed for immunohistochemical analysis. Kidney sections were marked with hematoxylin–eosin (Bio-Optica, Milan, Italy), and pancreas sections from organ donor were stained with monoclonal antibody anti-CD30 (Clone Ber-H2, Dako, Agilent Technologies, Santa Clara, CA, USA), and counterstained with hematoxylin. Stained sections were analyzed with color camera, which allows for scanning and digitizing sections with vertical multiple scans at 20x magnification (AperioScanscope, Leica, Wetzlar, Germany).
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