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2 protocols using polyethyleneimine pei branched mw 25 000 da

1

Cellulose-Graphene Oxide Composite Filter

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Whatman filter papers (N. 42, slow) with a reported mean pore size of 2.5 μm and a thickness of 0.39 mm were chosen as commercial cellulose filter papers. Zinc chloride, silver nitrate solution (0.1 M), sodium hydroxide, calcium hydroxide, ethylene glycol and dopamine hydrochloride were purchased from Merck Chemical Co. Polyethyleneimine (PEI, branched, MW 25,000 Da) was supplied by Sigma Aldrich Chemical Co. Graphene oxide (GO) sheets were bought from Platonic Nanotech Co.
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2

Characterization of PLGA-PEI Nanoparticles

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Poly(D,L-lactide-co-glycolide) (PLGA; Resomer RG 503H) was purchased from Sigma-Aldrich (Darmstadt, Germany) and used as received. Poly(ethylene imine) (PEI), branched, MW 25.000 Da was purchased from Sigma-Aldrich (Darmstadt, Germany) and used as 14 mg/mL stock solution in dimethyl sulphoxide (DMSO). Bovine Serum Albumin (BSA) and Superoxide Dismutase from bovine erythrocytes were purchased from Sigma-Aldrich (Dartmstadt, Germany) and used as received. Atto 488 NHS-ester, Atto 550 NHS-ester and Atto 633 amine were purchased from Atto-TEC GmbH (Siegen, Germany) and used as 10 mg/mL stock solution in DMSO. Fluorescently labelled PLGA was obtained as described elsewhere [16 (link)]. Lysotrack Red DND-99 and CellMask Green Plasma Membrane Stain were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and used as received. All other chemicals were purchased from Sigma Aldrich (Darmstadt, Germany) unless specified and used as received.
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