Plasma soluble inflammatory proteins were analyzed by multiplex (high sensitivity V-PLEX Human Biomarker 40-Plex Kit from MesoScale Discovery, Rockville, MD, USA). The analytes measured were C reactive protein, CCL11 (Eotaxin), CCL26 (Eotaxin-3), basic Fibroblast growth Factor (bFGF), Granulocyte-macrophage colony-stimulating factor (GM-CSF), Intercellular Adhesion Molecule (ICAM-1), Interferon (IFN)-γ, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, C-X-C Motif Chemokine Ligand (CXCL)-8, CXCL10, CCL2, CCL3, CCL4, CCL13, CCL22, Placental growth factor (PlGF), Serum Amyloid A, CCL17, Tyrosine kinase 2 (Tie)-2, TNF-α, TNF-β, Intercellular Adhesion Molecule (VCAM)-1, Vascular Endothelial growth factor (VEGF)-A, VEGF-C, VEGF-D, VEGF Receptor or Fms-like tyrosine kinase (Flt-1).
V plex human biomarker 40 plex kit
Manufactured by Mesoscale
Sourced in United States
The V-PLEX Human Biomarker 40-Plex Kit is a multiplex assay designed to measure the concentration of 40 different human biomarkers simultaneously in a single sample. The kit utilizes Mesoscale's electrochemiluminescence technology to provide quantitative results for the selected biomarkers.
Automatically generated - may contain errors
4 protocols using v plex human biomarker 40 plex kit
1
Multiplex Inflammatory Protein Analysis
2
Lung Biomarker Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All protein concentrations measured in perfusate were adjusted to the donor predicted total lung capacity as an estimate of perfused donor lung volume and reported as corrected perfusate concentrations (pg/ml), as previously described.7 (link) Lactate dehydrogenase levels were measured in perfusate, BAL, and tissue lysate as per manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL). Perfusate and tissue lysates were analyzed with a V-PLEX Human Biomarker 40-Plex Kit and a Human MMP 3-Plex Ultra-Sensitive Kit (both Meso Scale Diagnostics, LLC, Rockville, MD). BAL was analyzed using a V-PLEX human pro-inflammatory Panel 1 Kit and a Human MMP 3-Plex Ultra-Sensitive Kit (both Meso Scale Diagnostics, LLC). Human Syndecan-1 and endothelin-1 were measured using sandwich enzyme-linked immunosorbent assays (R&D Systems, Inc., Minneapolis, MN).
3
Plasma Biomarker Profiling in Angiogenesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium heparin‐anticoagulated plasma was collected on days 0, 12, and 26 and stored at −20°C until analysis. Proteins involved in angiogenesis, inflammation, and vascular injury were quantitatively measured using the V‐PLEX Human Biomarker 40‐Plex kit (Meso Scale Diagnostics, Rockville, MD) in accordance with manufacturer's instructions and included soluble VEGFR1 (sVEGFR1), vascular endothelial growth factors A, C, and D, (VEGF‐A, VEGF‐C, VEGF‐D), placental growth factor (PlGF), soluble angiopoietin receptor 2 (sTie2), cytokines, and adhesion molecules, for example, interferon gamma (IFNγ), tumor necrosis factor alfa (TNF‐α), TNF‐β, granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), interleukin (IL)‐1α, IL‐2, IL‐4, IL‐5, IL‐6, IL‐7, IL‐8 (HA), IL‐10, IL‐12 (p70), IL‐13, IL‐15, IL‐16, IL‐17, eotaxin and eotaxin‐3, interferon gamma‐induced protein 10 (IP‐10), methyl‐accepting chemotaxis protein 1 and 4 (MCP1/MCP4), C‐C motif chemokine 22 (MDC), macrophage inflammatory proteins 1α/1β (MIP‐1α/MIP‐1β), thymus and activation regulated chemokine (TARC), C‐reactive protein (CRP), serum amyloid A (SAA), soluble intercellular adhesion molecule‐1 (sICAM‐1/CD54), and soluble vascular adhesion molecule‐1 (sVCAM‐1). Plasma of healthy subjects served as controls.
4
Quantification of Plasma Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryopreserved plasma samples were used for quantification of inflammatory cytokines, chemokines and biomarkers using the V-PLEX Human Biomarker 40-Plex kit (MesoScale Diagnostics). Cell-free DNA levels were quantified using the Quanti-iT PicoGreen kit (Invitrogen) according to the manufacturer’s instructions and quantification of MPO–DNA complexes in plasma was performed as previously described55 (link). Differential abundance of 40-plex plasma inflammatory biomarkers was performed on the log2-transformed concentration values using the limma package v.3.48.3 in R, as previously described67 (link). Dimensionality reduction and visualization of the differences in plasma inflammatory mediators between patients with and without microbiota Enterobacteriaceae enrichment was performed using NMDS of mediator concentrations using the Vegan package v.2.6 in R. Figures showing log2FC of individual mediators between ICU patients with or without Enterobacteriaceae enrichment were calculated on absolute concentration using the limma package v.3.48.3 and plotted with ggplot2 . No clinical covariables were significantly associated with inflammatory mediator landscape composition (Supplementary Table 16 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!