Anti acetylated p53

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-acetylated p53 is a primary antibody that specifically recognizes the acetylated form of the p53 protein. P53 is a well-known tumor suppressor protein that plays a crucial role in cellular processes such as DNA repair, cell cycle regulation, and apoptosis. The acetylation of p53 is a post-translational modification that can affect its stability, localization, and transcriptional activity. This antibody can be used to detect and study the acetylated form of p53 in various experimental models and research applications.

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Lab products found in correlation

3 protocols using anti acetylated p53

Modulating MDM4 and p53 Acetylation

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Anti-MDM4 antibodies were purchased from Bethyl Laboratories. Anti-MDM2 antibody was purchased from R&D Systems. Anti–acetylated p53, anti-p53, anti–phospho ELK1, anti-ELK1, and anti–lamin B antibodies were from Cell Signaling. Anti-αSMA antibody was from American Research Products. Anti-Fas (CH11), anti-DD1α, anti-CD68, and anti–collagen I antibodies were from Thermo Fisher Scientific. Anti-AGE antibody was from Abcam. Anti-fibronectin and anti-GAPDH antibodies were from Santa Cruz Biotechnology. CXCL10 neutralizing antibody was from Thermo Fisher Scientific. CX3CL1 neutralizing antibody was from R&D Systems. The activities of commercial CXCL10 and CX3CL1 neutralizing antibodies were verified by functional blocking of cell chemotaxis. C646 and tamoxifen were from Sigma. CA was from MedChem Express. Validated MDM4 siRNAs were from OriGene.

Qu J., Yang S.Z., Zhu Y., Guo T., Thannickal V.J, & Zhou Y. (2021). Targeting mechanosensitive MDM4 promotes lung fibrosis resolution in aged mice. The Journal of Experimental Medicine, 218(5), e20202033.

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Biochemical Reagents and Antibody Sources

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All biochemical reagents were purchased from Millipore Sigma (St. Louis,
MO) and all cell culture reagents from Gibco (Thermo Fisher Scientific, Waltham,
MA) and Lonza (Walkersville, MD) unless specified otherwise. FLAG-HA-tagged
SirT1 was a kind gift from P. Puigserver (Harvard University, MA, USA). Protein
A/G beads (Santa Cruz, Dallas, TX) and antibodies were obtained from the
following companies: anti-GAPDH monoclonal antibody (Cell Signaling, Danvers,
MA; 1:1000), anti-β-Tubulin monoclonal antibody (Cell Signaling; 1:1000),
anti-HA antibody (Cell Signaling; 1:1000), anti-biotin antibody (Millipore
Sigma, St. Louis, MO; 1:2000), anti-HDAC1 (Cell Signaling, Danvers, MA; 1:1000),
anti-acetylated p53 (Cell Signaling, Danvers, MA 1:1000), anti-p53 (Cell
Signaling, Danvers, MA; 1:1000), anti-Glrx1 (Cayman Chemicals, Ann Arbor, MI;
1:1000), anti-SirT1 (Abcam, Cambridge, MA, 1 μg/ml) HA-magnetic beads
(Cell Signaling, Danvers, MA). HRP-conjugated secondary antibodies were from GE
Healthcare (Pittsburgh, PA) or eBioscience (San Diego, CA) TrueBlot system.
Antibody sources and dilutions are presented in Supplemental Table 1.

Rizvi S.H., Shao D., Tsukahara Y., Pimentel D.R., Weisbrod R.M., Hamburg N.M., McComb M.E., Matsui R, & Bachschmid M.M. (2021). Oxidized GAPDH transfers S-glutathionylation to a nuclear protein Sirtuin-1 leading to apoptosis. Free radical biology & medicine, 174, 73-83.

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Comprehensive Protein Analysis for Cancer Research

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Anti-MDM4 and anti-CD68 antibodies were obtained from ThermoFisher Scientific. Anti-acetylated p53, anti-p53, anti-fibronectin, anti-Collagen I, and anti-αSMA antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-DD1α and anti-Fas antibodies were purchased from Abcam (Cambridge, MA, USA). The anti-GAPDH antibody was from Abclonal (Wuhan, China). NSC146109 (XI-011) was purchased from Sigma-Aldrich. SJ172550 was obtained from MedChem Express (Monmouth Junction, NJ, USA).

Mei Q., Yang Z., Xiang Z., Zuo H., Zhou Z., Dong X., Zhang L., Song W., Wang Y., Hu Q., Zhou Y, & Qu J. (2023). Pharmacological inhibition of MDM4 alleviates pulmonary fibrosis. Theranostics, 13(9), 2787-2799.

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