Caspases-3/7 and -8 activities were determined by using a Caspase-Glo 3/7 or 8 assay kit (Promega) according to the manufacturer's instructions. In brief, 1.0 × 105 cells were seeded in 96-well plate with white wall (Nunc). After treatment, equal volume of Caspase-Glo 3/7 or 8 reagent was added to the cell culture medium, which had been equilibrated to room temperature for 1 h, cells were shaked for 5 min and incubated at room temperature for 30 min. Luminescent recording was performed with POLAR star Omega (BMG Labtech).
White wall
Manufactured by Thermo Fisher Scientific
The white wall is a sturdy and functional laboratory accessory designed to provide a clean, uniform surface for various laboratory applications. It serves as a neutral backdrop, allowing for clear visibility and presentation of lab equipment, samples, or experiments. The white wall is constructed with durable materials to withstand the demands of the laboratory environment.
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Lab products found in correlation
3 protocols using white wall
1
Caspase Activity Quantification
2
Evaluating Caspase Activities in Cells
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Caspase-1, caspase-3/7, and caspase-8 activities were determined by using a caspase-Glo 1 (G9951, Promega), caspase-Glo 3/7 (G8092, Promega), or caspase-8 assay kit (G8202, Promega) according to the manufacturer’s instructions. Cells were seeded in 96-well plate with white wall (Nunc). After treatment, an equal volume of caspase-Glo 1, caspase-Glo 3/7, or caspase-8 reagent was added to the cell culture medium and shaken for 30 min. Luminescent recording was performed with POLAR star Omega (BMG Labtech). For peritoneal lavage detection, 50 μl of assay reagent was added to peritoneal lavages of an equal volume isolated from mice by using 1 ml of PBS.
3
Caspase Activity Quantification
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Caspase-1, caspase-3/7, and caspase-8 activities were determined by using a caspase-Glo 1 (Promega, G9951), caspase-Glo 3/7 (G8092, Promega), or caspase-8 assay kit (G8202, Promega) according to the manufacturer's instructions. Cells were seeded in 96-well plate with white wall (Nunc). After treatment, an equal volume of caspase-Glo 1, caspase-Glo 3/7, or caspase-8 reagent was added to the cell culture medium and shaken for 30 min. Luminescent recording was performed with POLARstar Omega (BMG Labtech).
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