Anti atrx h 300

Manufactured by Santa Cruz Biotechnology

Anti-ATRX (H-300) is a primary antibody that recognizes the ATRX protein. ATRX is a chromatin remodeling protein involved in various cellular processes. This antibody can be used for applications such as Western blotting and immunohistochemistry to detect the ATRX protein.

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Lab products found in correlation

Anti sumo1, by Cell Signaling Technology (1 mentions) Ab3742, by Abcam (1 mentions) Ab4808, by Abcam (1 mentions) Anti sumo2 3, by Cell Signaling Technology (1 mentions) Anti h3, by Merck Group (1 mentions) Anti h2a, by Cell Signaling Technology (1 mentions) Anti ctcf, by Cell Signaling Technology (1 mentions) Protein a agarose beads, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

H-300 (126 citations) Anti-ATRX (12 citations)

2 protocols using anti atrx h 300

Comprehensive Immunofluorescence Antibody Panel

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The following primary antibodies were used:
Mouse Mab anti-mouse PML (mAb3739; Millipore), anti-human PML (clone 5E10; Roel van Driel or clone PG-M3; Santa Cruz), anti-SUMO-1 (clone 5B12; MBL), anti-SUMO-2/3 (clone 1E7; MBL), anti-NF160 (Invitrogen), anti-ßIII tubulin (MAB1637; Millipore), anti-ICP0 (Mab11060), anti-ICP4 (clone 10F1; Virusys), anti-ICP27 (Virusys); rabbit Mab anti-SUMO-1 (clone Y299; Abcam), anti-SUMO-2/3 (Mab4971; Cell Signaling): rabbit polyclonal anti-ATRX (H-300; Santa Cruz Biotechnology), anti-Daxx (M-112; Santa Cruz Biotechnology), anti-NF200 (Pierce), anti-SUMO-1 (4930; Cell Signaling), anti-SUMO-2/3 (ab3742; Abcam), anti-ICP0 (Rab190), anti-VP16 (ab4808; Abcam), and anti-pan-HSV-1 (LSBio) were used. All secondary antibodies were Alexa Fluor-conjugated and were raised in goats (Invitrogen).

Maroui M.A., Callé A., Cohen C., Streichenberger N., Texier P., Takissian J., Rousseau A., Poccardi N., Welsch J., Corpet A., Schaeffer L., Labetoulle M, & Lomonte P. (2016). Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment. PLoS Pathogens, 12(9), e1005834.

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ChIP-qPCR for Chromatin Profiling

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Chromatin immunoprecipitation (ChIP) was conducted as previously described (24 (link)) with anti-ATRX (H300; Santa Cruz Catalog #sc-15408), anti-CTCF (Cell Signaling Catalog #2899), anti-H3.3 (Millipore Catalog #17-10245) and anti-H2A (Cell Signaling Catalog #2578). DNA–antigen complexes were retrieved by incubation with protein A agarose beads (Cell Signaling). Quantification was conducted as previously described (24 (link)).

Kernohan K.D., Vernimmen D., Gloor G.B, & Bérubé N.G. (2014). Analysis of neonatal brain lacking ATRX or MeCP2 reveals changes in nucleosome density, CTCF binding and chromatin looping. Nucleic Acids Research, 42(13), 8356-8368.

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