ECFCs were isolated from canine peripheral blood samples (20 mL). Mononuclear cells (MNCs) were acquired via a canine peripheral blood mononuclear cell extraction kit (Solarbio, China) using density gradient fractionation, followed by re-suspension in complete medium (EGM-2; Lonza, USA) containing 10% FBS. They were next cultured on fibronectin-coated T25 cell dishes at 37 °C in a 5% CO2 humid chamber. The ECFC phenotypes were assessed via flow cytometry, upon addition of antibodies against mouse CD34, CD45 CD105, and CD133 (Invitrogen, USA). Dil-ac-LDL uptake and FITC-UEA-1 binding assays were next performed with corresponding kits (Invitrogen, USA), following kit guidelines, to further examine the profiles of ECFC.
Cd45 cd105
Manufactured by Thermo Fisher Scientific
Sourced in United States
CD45 CD105 is a laboratory reagent used for the detection and identification of certain types of cells. It is a combination of two monoclonal antibodies that target the CD45 and CD105 surface markers. This product can be used in flow cytometry and other analytical techniques to assist in the characterization of cell populations.
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Lab products found in correlation
2 protocols using cd45 cd105
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Isolation and Characterization of Canine ECFCs
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Isolation and Characterization of Canine ECFC
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Canine peripheral blood samples (40 mL) were used to isolate mononuclear cells (MNCs) via canine peripheral blood mononuclear cell isolation kit (Solarbio, China) density gradient centrifugation, after which MNCs were cultured on fibronectin-coated T25 cell dishes containing EGM2 medium (EGM-2; Lonza, USA) at 37 °C in a 5%CO2 humidified incubator. The phenotypes of ECFC were examined via flow cytometry using antibodies against mouse CD31, CD34, CD45 CD105, and CD133 (Invitrogen, USA). Dil-ac-LDL uptake and FITC-UEA-1 binding assays were conducted to further investigate the characteristics of ECFC.
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