Hpp750life

Manufactured by Memmert

Sourced in Germany

The HPP750Life is a high-pressure processing (HPP) system designed for laboratory applications. It is capable of subjecting samples to high pressure up to 7500 bar (750 MPa) for research and testing purposes. The system is equipped with a cylindrical pressure chamber and can accommodate various sample sizes.

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Lab products found in correlation

C57bl 6nrj mice, by Janvier Labs (2 mentions) Homeothermic monitor, by Harvard Apparatus (1 mentions) Cl316 243, by Bio-Techne (1 mentions) Adiponectin cre mice, by Jackson ImmunoResearch (1 mentions) Ucp1 mice, by Jackson ImmunoResearch (1 mentions)

10 protocols using hpp750life

Adaptive Thermogenesis in Mice

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During cold acclimation, mice were housed in groups of 2–3 individuals in type II cages (370 cm², Tecniplast GmbH) with bedding material. Cages were transferred to a climate cabinet (HPP750life, Memmert GmbH + Co. KG) controlling ambient temperature and relative humidity. Mice were maintained in a 12 h light/dark cycle. Ambient temperature was decreased stepwise in weekly intervals from 23 °C to 18 °C, 15 °C, 10 °C and 5 °C. Cold exposure experiments started at the age of 10 weeks and lasted 4 weeks. Control mice were kept in a climate cabinet continuously maintained at 23 °C (HPP750life, Memmert GmbH + Co. KG).
The β₃-adrenergic receptor agonist CL316,243 (Tocris, Bio-Techne GmbH) was injected intraperitoneally in mice at 13 weeks of age for 5 consecutive days at a daily dose of 1.0 mg/kg. Controls received vehicle injections with saline solution (0.9% NaCl, B. Braun Melsungen AG). Two days after the last CL316,243 injection, LUC bioluminescence was imaged in anesthetized mice. Afterwards mice were killed and tissues dissected for further analyses.

Wang H., Willershäuser M., Karlas A., Gorpas D., Reber J., Ntziachristos V., Maurer S., Fromme T., Li Y, & Klingenspor M. (2018). A dual Ucp1 reporter mouse model for imaging and quantitation of brown and brite fat recruitment. Molecular Metabolism, 20, 14-27.

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Murine Cold Exposure Adipose Modeling

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All of the animal studies were conducted with approved protocols from the Danish Animal Experiments Inspectorate (permit numbers 2014-15-0201-00181 and 2015-15-0201-00728) and the University of Copenhagen (project numbers P14-379, P16-010, and P16-021). Male wild-type C57Bl/6NRj mice (Janvier) were used for cold exposure studies. Prior to the experimental procedures, the mice were group housed and acclimatized to our housing unit on a 12:12 h light/dark cycle (lights on at 6:00 and off at 18:00) with ad libitum access to chow food (1310, Altromin) and water. For cold exposure experiments, 12- to 16-week-old mice were individually housed and placed in climate-controlled rodent incubators (HPP750Life, Memmert) at 29 °C to acclimate to thermoneutrality for three weeks. Experimental groups were then moved to an incubator set to 5 °C and kept for 3, 8, 24 h, 1, and 3 weeks until dissection (n = 5 was used per time point). The mice were euthanized by neck dislocation; scWAT, BAT, and vWAT were collected at dissection and immediately snap frozen in liquid nitrogen, fixed in 4% formalin for histology (see the method section on immunohistochemistry), or digested to obtain the stroma-vascular fraction (SVF) and mature adipocyte fraction (MAF) of scWAT (see the Methods section on digestion of the whole scWAT for isolation of SVF and MAF).

Rabiee A., Plucińska K., Isidor M.S., Brown E.L., Tozzi M., Sidoli S., Petersen P.S., Agueda-Oyarzabal M., Torsetnes S.B., Chehabi G.N., Lundh M., Altıntaş A., Barrès R., Jensen O.N., Gerhart-Hines Z, & Emanuelli B. (2020). White adipose remodeling during browning in mice involves YBX1 to drive thermogenic commitment. Molecular Metabolism, 44, 101137.

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Perinatal Stress and Immune Challenge

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For the induction of perinatal restraint stress, pregnant mice were placed into ventilated 50 ml conical tube for 3 hours/day during E12.5-E17.5. For perinatal cold exposure, pregnant mice or nursing mice with pups were placed 5 °C in cold chamber (HPP750Life, Memmert) 6–12 hours/day with 12 hours light/dark condition. Control mice for cold exposure were placed in room temperature with 12 hours light/dark condition. For poly (I:C) injection, we injected either saline or poly (I:C) (20 μg in 10 μl; High Molecular weight, Invivogen) intraperitoneally on PND3 directly to pups.

Hong J.Y., Lim J., Carvalho F., Cho J.Y., Vaidyanathan B., Yu S., Annicelli C., Eddie W.K, & Medzhitov R. (2020). Long-term programming of CD8 T cell immunity by perinatal exposure to glucocorticoids. Cell, 180(5), 847-861.e15.

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Thermoregulation in Muscle-specific Myoglobin Knockout Mice

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Whole‐body Mb‐KO mice on the NMRI background were previously generated²⁹, ⁴⁹ and bred at the Sächsische Inkubator für Klinische Translation (SIKT), Leipzig. NMRI WT controls were obtained from Janvier (Saint Berthevin, France), and kept in the local animal house for one week for acclimatization. C57BL/6N mice were bred at the SIKT, Leipzig. All mice were housed in pathogen‐free facilities (3‐5 mice per group and cage) at 23°C on a 12 h light/dark cycle, as indicated. All mice were fed a standard chow diet (EV153, 3.3% from fat, Ssniff^®, Soest, Germany) and had ad libitum access to water and food, except when indicated. Male NMRI WT and Mb‐KO mice (10‐12 weeks of age) were then adapted to single housing and subsequently housed in a climate chamber at either 30°C, 23°C or 8°C, respectively, for one week. Body temperature was measured after 5 days. After 7 days, BAT and tail surface temperatures were measured by thermal imaging (VarioCAM^® hr, Infratec, Dresden, Germany) as previously described.⁵² Acute cold‐tolerance test was done with single‐housed mice, which were transferred from 30°C to a climate chamber (Memmert HPP750 life) at 8°C for 6 h and rectal body temperature was measured every hour.

Christen L., Broghammer H., Rapöhn I., Möhlis K., Strehlau C., Ribas‐Latre A., Gebhardt C., Roth L., Krause K., Landgraf K., Körner A., Rohde‐Zimmermann K., Hoffmann A., Klöting N., Ghosh A., Sun W., Dong H., Wolfrum C., Rassaf T., Hendgen‐Cotta U.B., Stumvoll M., Blüher M., Heiker J.T, & Weiner J. (2022). Myoglobin‐mediated lipid shuttling increases adrenergic activation of brown and white adipocyte metabolism and is as a marker of thermogenic adipocytes in humans. Clinical and Translational Medicine, 12(12), e1108.

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Cold Tolerance Test in Mice

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Mice were pair-housed at 22°C. Food was withdrawn at the commencement of the cold tolerance test as animals were transferred to new cold acclimated cages containing only bedding material. The cages were then placed into climate controlled rodent incubators (Memmert HPP750Life) set to 5°C. Core body temperature was obtained with a Homeothermic Monitor (Harvard Apparatus) by gently inserting a thermal probe into the mouse rectum.

Sustarsic E.G., Ma T., Lynes M.D., Larsen M., Karavaeva I., Havelund J.F., Nielsen C.H., Jedrychowski M.P., Moreno-Torres M., Lundh M., Plucinska K., Jespersen N.Z., Grevengoed T.J., Kramar B., Peics J., Hansen J.B., Shamsi F., Forss I., Neess D., Keipert S., Wang J., Stohlmann K., Brandslund I., Christensen C., Jørgensen M.E., Linneberg A., Pedersen O., Kiebish M.A., Qvortrup K., Han X., Pedersen B.K., Jastroch M., Mandrup S., Kjær A., Gygi S.P., Hansen T., Gillum M.P., Grarup N., Emanuelli B., Nielsen S., Scheele C., Tseng Y.H., Færgeman N.J, & Gerhart-Hines Z. (2018). Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis. Cell Metabolism, 28(1), 159-174.e11.

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Cold Adaptation in C57BL/6J Mice

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Eight-week-old male C57BL/6J mice were housed in climate cabinets (HPP750 life, Memmert) at 23°C and 55% humidity with a 12/12 h light/dark cycle. Mice were provided ad libitum access to water and a control diet (Ssniff, Cat# S5745-E720). After an adaptation phase of 3 weeks, mice were assigned to one of two groups and transferred to preconditioned cabinets at 5 or 30°C. After 1 week, mice were killed by CO₂ exposure and tissues were immediately dissected, snap frozen in liquid nitrogen, and stored at −80°C until further processing. The experiment was performed according to the German animal welfare law with permission from the district government of Upper Bavaria (Regierung von Oberbayern, reference number ROB-55.2-2532.Vet_02-16-166).

Dieckmann S., Maurer S., Fromme T., Colson C., Virtanen K.A., Amri E.Z, & Klingenspor M. (2020). Fatty Acid Metabolite Profiling Reveals Oxylipins as Markers of Brown but Not Brite Adipose Tissue. Frontiers in Endocrinology, 11, 73.

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Cold Exposure Effects on Mice

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All animal studies were performed with approved protocols from the The Danish Animal Experiments Inspectorate permit number 2014-15-0201-00181 and the University of Copenhagen project number P14-379 and P16-021. Male wild-type C57Bl/6NRj mice (Janvier) were used for cold exposure studies and primary preadipocyte cultures. Unless otherwise noted, mice were group housed on a 12-hour light/dark cycle (lights on at 6:00 and off at 18:00) and given ad libitum access to chow food (Altromin, 1310). For cold exposure experiments, twelve-sixteen week old mice were individually housed and placed into climate controlled rodent incubators (Memmert HPP750Life) set to 29°C to acclimate to thermoneutrality for two weeks. Cold exposure mice were then moved to an incubator set to 5°C and kept there until dissection.

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Dietary Effects on C57BL/6 Mice

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Seven-week old male C57BL/6 mice (n = 18, weight = 24.9 ± 1.7 g) were housed in groups of three per cage in a climate controlled chamber (HPP750life Memmert GmbH + Co. KG, Schwabach, Germany). A 12 h light:dark cycle (6 a.m.–6 p.m.) was maintained throughout the housing period and the mice had access to ad libitum standard chow and water. The mice were purchased in two separate batches from the same vendor (Janvier labs, Le Genest-Saint-Isle, France). The Danish Expectorate of Animal Experiments approved all animal experiments (project identification code 2015-15-0201-00709, date 07-12-2015).

Riis-Vestergaard M.J., Breining P., Pedersen S.B., Laustsen C., Stødkilde-Jørgensen H., Borghammer P., Jessen N, & Richelsen B. (2018). Evaluation of Active Brown Adipose Tissue by the Use of Hyperpolarized [1-13C]Pyruvate MRI in Mice. International Journal of Molecular Sciences, 19(9), 2597.

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Adiponectin-Cre Murine Cold Exposure Protocol

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All mice were on a C57BL/6 J background. Adiponectin-Cre mice were from the Jackson Laboratory (The Jackson Laboratory, Bar Harbor, ME; USA) and Mllt4^flox/flox mice were provided by Pr. Müller^{23 (link)}. Mice were housed at the Department of Experimental Medicine, Faculty of Health Sciences, at the University of Copenhagen at 22 °C under daily 12 h light/dark cycles in ventilated racks, with free access to food and water, and cages changed every 2 weeks^{13 (link)}. For cold exposure experiments, 12–16 weeks old mice were individually housed and placed into climate controlled rodent incubators (Memmert HPP750Life) at 29 °C to acclimate to thermoneutrality for 14–18 days. Cold-exposed mice were then moved to an incubator set to 5 °C with food and water ad libitum, until termination and dissection. All animal experiments were approved by the Danish Animal Experiments Inspectorate (permit number 2015-15-0201-00728), and performed in accordance with the guidelines and regulations of the Department of Experimental Medicine at the University of Copenhagen.

Lundh M., Altıntaş A., Tozzi M., Fabre O., Ma T., Shamsi F., Gerhart-Hines Z., Barrès R., Tseng Y.H, & Emanuelli B. (2021). Cold-induction of afadin in brown fat supports its thermogenic capacity. Scientific Reports, 11, 9794.

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UCP-1 Deficient Mice: Breeding and Phenotyping

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Heterozygous UCP-1 mice were purchased from The Jackson Laboratory (stock #003124) and used for breeding of UCP-1 knock-out (KO) mice. Male and female wild-type (WT) and UCP-1 KO littermates were used for all experiments on UCP-1 deficiency. Breeding occurred at regular housing temperature (23°C), and offspring were weaned at postnatal day 21. After weaning, mice were housed with same-sex littermates either at regular housing temperature (23°C) or at thermoneutrality (29°C) using an environmental chamber (HPP750life, Memmert) for temperature control. All mice were maintained at a regular 12 h light/dark cycle (lights on at 8:00 A.M.) and food (regular chow diet) and water available were ad libitum unless indicated otherwise. For FGF-21 overexpression experiments, C5BL6/N mice were purchased from Charles River. All animal experiments were conducted in agreement with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines and the U.K. Animals (Scientific Procedures Act, 1986) and associated guidelines (EU Directive 2010/63/EU for animal experiments) and were approved by the national ethical committee on animal care and use (Bundesministerium für Wissenschaft und Forschung: BMBWF-66.009/0175-V/3b/2019).

Sideromenos S., Gundacker A., Nikou M., Oberle R., Horvath O., Stoehrmann P., Partonen T, & Pollak D.D. (2022). Uncoupling Protein-1 Modulates Anxiety-Like Behavior in a Temperature-Dependent Manner. The Journal of Neuroscience, 42(40), 7659-7672.

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