The ChIP assay was carried out using the EZ-ChIP Kit (Millipore, USA) following the manufacturer's protocol. Briefly, cardiomyocytes were treated with formaldehyde and incubated for 10 minutes to generate DNA-protein cross-links. Cell lysates were then sonicated to generate chromatin fragments of 200-300 bp and immunoprecipitated with EZH2 and H3K27me3-specific antibodies (Millipore, USA) or negative control IgG (Millipore, USA). The precipitated chromatin DNA was purified and subjected to quantitative PCR analysis for enrichment of the target sequences.
Ezh2 and h3k27me3 specific antibodies
Manufactured by Merck Group
Sourced in United States
EZH2 and H3K27me3-specific antibodies are laboratory reagents used for the detection and quantification of epigenetic modifications in cellular samples. These antibodies specifically recognize the histone methyltransferase EZH2 and the histone H3 lysine 27 trimethylation (H3K27me3) mark, which are associated with gene repression and chromatin compaction. The antibodies can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunofluorescence, to study the roles of these epigenetic regulators in cellular processes.
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3 protocols using ezh2 and h3k27me3 specific antibodies
1
ChIP Assay for EZH2 and H3K27me3
2
ChIP Assay for EZH2 and H3K27me3
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The ChIP assay was performed using the EZ-ChIP Kit (Millipore, USA). Briefly, cardiomyocytes were treated with formaldehyde and incubated for 10 minutes to generate DNA-protein cross-links. Cell lysates were sonicated to generate chromatin fragments of 200–300 bp and immunoprecipitated with EZH2 and H3K27me3 -specific antibodies (Millipore, USA). The precipitated chromatin DNA was purified and subjected to quantitative PCR analysis for enrichment of the target sequences.
3
ChIP Assay for EZH2 and H3K27me3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChIP assays were conducted by using an EZ-CHIP kit according to the manufacturer’s protocol (Millipore, Burlington, MA, USA). SW620 cells were incubated with formaldehyde for 10 min, and then sonicated to obtain cell lysates. The cell lysates were immunoprecipitated with EZH2- and H3K27me3-specific antibodies (Millipore); IgG was used as a control. The precipitated chromatin DNA was analyzed by qPCR.
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