Naive CD4+CD45+ T cells were purified by negative selection (Invitrogen) and cultured with allogeneic myelin-stimulated DCs in 24-well plates for 6 d. Primed T cells were recovered, washed, and allowed to rest for 3 d in the presence of IL-7 and IL-15 (ImmunoTools). After 9 d of the primary stimulation, primed T cells were washed and restimulated by co-culturing them with LPS-activated moDC from the original donor at different ratios. After 6 d, plates were pulsed with [3H]thymidine (1 µCi/well; GE Healthcare) for an additional 18 h and [3H]thymidine incorporation was measured on a Betaplate 1205 liquid scintillation counter (Wallac-LKB).
Negative selection
Manufactured by Thermo Fisher Scientific
Negative selection is a laboratory technique used to isolate a specific cell population from a heterogeneous sample by removing unwanted cells. It involves the use of antibodies or other targeting agents to identify and eliminate cells with specific surface markers or characteristics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using negative selection
1
Allogeneic T Cell Proliferation Assay
2
Allogeneic T Cell Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FhTE/LPS-stimulated mo-DCs were washed and incubated for 6 days with allogeneic naive CD4+CD45+ T cells that were previously purified by negative selection (Invitrogen). Primed T cells were recovered, washed, and allowed to rest for 3 d in the presence of IL-7 and IL-15 (ImmunoTools). After 9 d of the primary stimulation, primed T cells were washed and restimulated by co-culturing them with LPS-activated moDC from the original donor at different ratios. After 6 d, plates were pulsed with [3H]thymidine (1 μCi/well; GE Healthcare) for an additional 18 h and [3H]thymidine incorporation was measured on a Betaplate 1205 liquid scintillation counter (Wallac-LKB).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!