Swine anti rabbit immunoglobulins hrp

Manufactured by Agilent Technologies

Sourced in Germany, Denmark

Swine Anti-Rabbit Immunoglobulins/HRP is a laboratory reagent used for the detection and quantification of rabbit immunoglobulins in various applications. It contains swine-derived antibodies conjugated with horseradish peroxidase (HRP), which allows for colorimetric or chemiluminescent detection.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 568, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) γ h2ax 2577, by Cell Signaling Technology (1 mentions) Anti flag 2368, by Cell Signaling Technology (1 mentions) Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg h l cross adsorbed secondary antibody, by Thermo Fisher Scientific (1 mentions) Nystatin dihydrate, by Merck Group (1 mentions) Goat anti rabbit immunoglobulins hrp, by Agilent Technologies (1 mentions) Phalloidin tritc, by Merck Group (1 mentions) Daf fm da, by Thermo Fisher Scientific (1 mentions) Celltracker red cmtpx dye, by Thermo Fisher Scientific (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Mms 435p, by Fortrea (1 mentions) Poly d lysine, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Complete protease inhibitor cocktail tablet, by Roche (1 mentions) Torin 1, by Cayman Chemical (1 mentions) Phospho nf kb p65, by Cell Signaling Technology (1 mentions) Srankl, by Thermo Fisher Scientific (1 mentions)

6 protocols using swine anti rabbit immunoglobulins hrp

Comprehensive Antibody Inventory for DNA Damage Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-FLAG (#2368) and γH2AX (#2577) antibodies were purchased from Cell Signaling Technology, whereas antibodies for BRCA1 (#sc-6954), H1.4 (#sc-393358), RNF8 (#sc-2714620), and GFP (#sc-9996) were from Santa Cruz Biotechnology. Antibodies against α-tubulin (GTX628802) were obtained from GeneTex. Anti-RPA (ab2175), H1.0 (ab11079), H1.2 (ab17677), H1.3 (ab183736), and H1.4 (ab18208) were purchased from Abcam, whereas anti-H1.1 was from Insight Biotechnology (GTX117055). Finally, anti-RPA pS4+pS8 (A300-245A) antibodies were purchased from Bethyl, whereas anti-H3 antibodies were obtained from Sigma-Aldrich (05-928). Goat anti-mouse immunoglobulins/HRP (#P044701-2) and swine anti-rabbit immunoglobulins/HRP (P039901-2) were purchased from Dako. Goat anti-mouse IgG (H + L) secondary antibody, Alexa Fluor 488 (#A10680) and goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, and Alexa Fluor 568 (#A11011) were purchased from Thermo Fisher Scientific.

Ozgencil M., Dullovi A., Christiane Higos R.C., Hořejší Z, & Bellelli R. (2023). The linker histone H1–BRCA1 axis is a crucial mediator of replication fork stability. Life Science Alliance, 6(9), e202301933.

+ Open protocol

+ Expand

Immunostaining and Imaging of Akt Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: Mouse Anti-IIIβ-tubulin (MMS-435P, Covance), Rabbit mAb Anti-P-Akt (Ser473; #4060, Cell Signaling), Goat Anti-Akt (C-20; sc-1618, Santa Cruz), Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-21202, Thermo Fisher), Swine Anti-Rabbit Immunoglobulins/HRP (P0217, Dako), Rabbit Anti-Goat Immunoglobulins/HRP (P0449, Dako).
The following drugs and reagents were used: Poly-D-Lysine (P7280, Sigma), rat tail collagen Type I, Rat Tail (354236, Corning), Nystatin dihydrate (N4014, Sigma), DMSO (D5879, Sigma), Methyl-β-cyclodextrin (C4555, Sigma), Phalloidin—TRITC (P1951, Sigma), NG-Monomethyl-L-arginine, monoacetate salt (L-NMMA; ab120137, Abcam), diamino-fluorescein Diacetate (DAF-FM DA; D-23844, Molecular Probes), CellTracker™ RedCMTPX Dye (C34552, Thermo Fisher), Complete Protease Inhibitor Cocktail Tablets (11697498001, Roche), MK-2206-2HCl (A10003, AdooQ Bioscience).

Roselló-Busquets C., Hernaiz-Llorens M., Soriano E, & Martínez-Mármol R. (2020). Nystatin Regulates Axonal Extension and Regeneration by Modifying the Levels of Nitric Oxide. Frontiers in Molecular Neuroscience, 13, 56.

+ Open protocol

+ Expand

Western Blot Detection of Hcp and NifH Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were separated on 12.5% SDS–PAGE gels (Laemmli, 1970 (link)) and transferred to a nitrocellulose membrane (Amersham^TM Protran^®, Sigma-Aldrich, Munich, Germany). For detection of Hcp proteins, membranes were blocked in 1× TBS containing 5% skim milk, incubated overnight in 1× TBS with 1% BSA containing 1:3000 rabbit polyclonal antisera against A. olearius strain BH72 Hcp proteins (Shidore et al., 2012 (link)), followed by a 3 h incubation in 1× TBS supplemented with 1% BSA containing 1:10000 polyclonal swine anti-rabbit immunoglobulins/HRP (Dako, Hamburg, Germany). For detection of NifH proteins, similar conditions were used except for using antiserum against NifH of R. rubrum, kindly provided by R. Ludden (Berkeley, CA, United States).

Jiang X., Beust A., Sappa P.K., Völker U., Dinse T., Herglotz J, & Reinhold-Hurek B. (2019). Two Functionally Deviating Type 6 Secretion Systems Occur in the Nitrogen-Fixing Endophyte Azoarcus olearius BH72. Frontiers in Microbiology, 10, 459.

+ Open protocol

+ Expand

Evaluating M-CSF, RANKL, and TNF Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human and murine M-CSF, sRANKL, and TNF were purchased from Peprotech (Rocky Hill, NJ, USA). Inhibitors, SP2509, RN-1, ORY-1001, and Torin1, were purchased from Cayman Chemical Company (Ann Arbor, MI). The antibodies used were as follows: LSD1, phospho-NFkB p65, Phospho-4E-BP1, HIF1a, E2F1, PHD-2 (Cell Signaling Technologies, Danvers, MA), p38, c-Myc (BioLegend, San Diego, California) and Swine Anti-Rabbit Immunoglobulins/HRP (DAKO, Hovedstaden, Denmark).

Doi K., Murata K., Ito S., Suzuki A., Terao C., Ishie S., Umemoto A., Murotani Y., Nishitani K., Yoshitomi H., Fujii T., Watanabe R., Hashimoto M., Murakami K., Tanaka M., Ito H., Park-Min K.H., Ivashkiv L.B., Morinobu A, & Matsuda S. (2022). LSD1 metabolically integrates osteoclast differentiation and inflammatory bone resorption through HIF-1α and E2F1. Arthritis & rheumatology (Hoboken, N.J.), 74(6), 948-960.

+ Open protocol

+ Expand

Immunoblotting Protocol for FANCA and β-Actin

Check if the same lab product or an alternative is used in the 5 most similar protocols

The FANCA antibody (Cell Signalling, D1L2Z) was used at 1:1000 in 5% w/v BSA, 1× TBS, 0.1% Tween-20 at 4 °C with gentle shaking, overnight. The β-actin antibody (Abcam, 8227) was used at 1:3,000 in the same conditions. Swine anti-rabbit immunoglobulins HRP (Dako) was used as secondary antibody at 1:2,000 for 1 h at room temperature.

Garaycoechea J.I., Crossan G.P., Langevin F., Mulderrig L., Louzada S., Yang F., Guilbaud G., Park N., Roerink S., Nik-Zainal S., Stratton M.R, & Patel K.J. (2018). Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature, 553(7687), 171-177.

+ Open protocol

+ Expand

Immunoblotting Analysis of Protein Abundance

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were homogenized in RIPA buffer, and equal amounts of proteins were separated on SDS-PAGE under reducing conditions, and transferred to a nitrocellulose membrane (Whatman). Proteins of interest were detected using the following antibodies: OXCT1 (ab105320; Abcam), ACAT1 (HPA004428; Sigma), eEF2 (2332; Cell signaling), Polyclonal Swine Anti-Rabbit Immunoglobulins/HRP (P0399, Dako). Densitometric analysis of immunoblots was performed on 6 individual samples using Image-J software, and a representative selection from this group is presented in each figure.

Svensson K., Albert V., Cardel B., Salatino S, & Handschin C. (2016). Skeletal muscle PGC-1α modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(5), 1976-1986.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!