HEK293T and BT20 cells were purchased from American Type Culture Collection (ATCC) in 2012 and 2013, respectively. They have not been authenticated since the purchase. They were cultured in DMEM/High Glucose medium (GenDepot) supplemented with 10% fetal bovine serum plus antibiotic-antimycotic solution (GenDepot). TLM-HMECs (genetically engineered human mammary epithelial cells) have not been authenticated since obtained from Dr. Thomas Roberts in 2003 (37 (link)) and were cultured in mammary epithelial growth medium (MEGM, Lonza). All cell lines used in this study were grown in a humidified incubator at 37°C with 5% CO2 and mycoplasma free as periodically tested by Universal Mycoplasma Detection Kit (ATCC).
Dmem high glucose medium
Manufactured by GenDEPOT
DMEM/High Glucose medium is a commonly used cell culture medium that provides essential nutrients and components required for the growth and maintenance of a wide range of cell types. It contains a high concentration of glucose as the primary energy source.
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2 protocols using dmem high glucose medium
1
Cell Line Validation and Maintenance
2
Cell Line Cultivation and Authentication
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HEK293T and BT20 cells were purchased from ATCC in 2012 and 2013, respectively. They have not been authenticated since the purchase. They were cultured in DMEM/High Glucose medium (GenDepot) supplemented with 10% FBS plus antibiotic–antimycotic solution (GenDepot). TLM-HMECs (genetically engineered human mammary epithelial cells) have not been authenticated since they were obtained from Dr. Thomas Roberts (Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA) in 2003 (37 (link)) and were cultured in mammary epithelial growth medium (MEGM, Lonza). All cell lines used in this study were grown in a humidified incubator at 37°C with 5% CO2 and Mycoplasma free as periodically tested by Universal Mycoplasma Detection Kit (ATCC).
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