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Lc msd 1100 series

Manufactured by Agilent Technologies
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The LC-MSD 1100 series is a liquid chromatography-mass spectrometry (LC-MS) system manufactured by Agilent Technologies. It is designed to perform qualitative and quantitative analysis of chemical compounds. The system combines a liquid chromatography module with a mass spectrometer, enabling the separation, identification, and quantification of complex mixtures.

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Lab products found in correlation

Bond elut c18, by Agilent Technologies (2 mentions) Human leptin, by Merck Group (1 mentions) Cobas e411 analyzer, by Roche (1 mentions)

3 protocols using lc msd 1100 series

1

Endocannabinoid Profiling in Serum

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Serum concentrations of the endocannabinoids AEA were extracted from serum using Bond Elut C18 solid phase extraction columns (Varian Inc, Lake Forest, CA), as previously described (Hill et al., 2008 (link)). These extractions for AEA were done concurrent to those previously reported for 2-AG, on the same samples. The lipid was quantified in the lipid extracts by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS; Agilent LC-MSD 1100 series) and quantified by isotope dilution as described previously (Patel et al., 2005 (link)). Congeners of AEA, the structural analogs oleoylethanolamide (OEA) and palmitoylethanolamide (PEA) were simultaneously assayed.
, & Hanlon E.C. (2019). Impact of circadian rhythmicity and sleep restriction on circulating endocannabinoid (eCB) N-arachidonoylethanolamine (anandamide). Psychoneuroendocrinology, 111, 104471.
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2

Quantifying Endocannabinoids in Tissue

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Concentrations of the endocannabinoids N-arachidonylethanolamine (AEA) and 2-arachidonylglycerol (2-AG) were extracted from tissue using a protocol adapted from Patel et al. 2003 (link). Briefly, tissue samples were homogenized in 2mL of acetonitrile with 84 pmol [2H8]AEA and 186pmol [2H8]2-AG using glass rods. Samples were sonicated for 60 min and frozen overnight at −20°C to precipitate proteins. The supernatant was obtained following centrifugation at 1500 × g for 2 min at 4°C, was dried under N2 and the lipids were resuspended in methanol. The two endocannabinoids were isolated and quantified by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS; Agilent LC-MSD 1100 series) and quantified by isotope dilution as described previously (Patel et al. 2003 (link)).
McReynolds J.R., Doncheck E.M., Vranjkovic O., Ganzman G.S., Baker D.A., Hillard C.J, & Mantsch J.R. (2015). CB1 receptor antagonism blocks stress-potentiated reinstatement of cocaine seeking in rats. Psychopharmacology, 233(1), 99-109.
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3

Quantification of Endocannabinoids and Hormones in Neuroimaging Study

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Upon arrival at the imaging center, an MRI safe catheter was placed in participant’s left arm and samples were collected through antecubital venipuncture. Blood plasma samples were collected at baseline (before dinner) and at four time points following the dinner. Blood serum samples for ECS analysis were only collected at 7:30 pm while subjects were inside the MRI scanner, 90 min after the initiation of dinner (in between the 2nd and 3rd fMRI run). Prior to drawing each sample, 1.5–2 mL of blood were drawn off to remove potentially diluted blood from the dead space of the catheter. Samples were put on ice, centrifuged, aspirated, divided into aliquots, and stored at −80°C until assay.
Serum levels of 2-arachidonoylglycerol (2-AG) and 2-oleoylglycerol (2-OG) were extracted using the Bond Elut C18 solid-phase extraction columns (1 ml; Varian Inc, Lake Forest, CA). Serum samples were processed and the two compounds were quantified using chemical ionization liquid chromatography/mass spectrometry (LC-ESI-MS; Agilent LC-MSD 1100 series, Ramsey, MN), as previously described (Patel et al., 2005 (link)).
We used enzyme-linked immunosorbent assay (ELISA) kits for total plasma ghrelin (human ghrelin, Millipore), plasma leptin (human leptin, Millipore), and cobas e411 analyzer (Roche) for plasma insulin, and serum cortisol.
Bhutani S., Howard J.D., Reynolds R., Zee P.C., Gottfried J, & Kahnt T. (2019). Olfactory connectivity mediates sleep-dependent food choices in humans. eLife, 8, e49053.
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